Search results for the GEO ID: GSE27799 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM686723 | GPL1261 |
|
LSK WT replicate1b
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Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old WT mouse
|
gender: male
age: 5 weeks
genotype: Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686723
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686723/suppl/GSM686723.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
GSM686724 | GPL1261 |
|
LSK WT replicate2b
|
Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old WT mouse
|
gender: female
age: 5 weeks
genotype: Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686724
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686724/suppl/GSM686724.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
GSM686725 | GPL1261 |
|
LSK WT replicate3
|
Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old WT mouse
|
gender: male
age: 5 weeks
genotype: Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686725
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686725/suppl/GSM686725.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
GSM686726 | GPL1261 |
|
LSK N1IC replicate1
|
Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old N1-IC+ mouse
|
gender: female
age: 5 weeks
genotype: lox-stop-lox N1-IC Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686726
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686726/suppl/GSM686726.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
GSM686727 | GPL1261 |
|
LSK N1IC replicate2
|
Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old N1-IC+ mouse
|
gender: female
age: 5 weeks
genotype: lox-stop-lox N1-IC Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686727
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686727/suppl/GSM686727.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
GSM686728 | GPL1261 |
|
LSK N1IC replicate3
|
Lineage negative, cKit+, Sca1+ bone marrow cells from 5 weeks old N1-IC+ mouse
|
gender: female
age: 5 weeks
genotype: lox-stop-lox N1-IC Mx1-cre+
|
2 weeks after polyIC injection
|
Sample_geo_accession | GSM686728
| Sample_status | Public on May 12 2011
| Sample_submission_date | Mar 07 2011
| Sample_last_update_date | May 12 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 21 days old Ncstn flox/flox and Ncstn flox/flox Mx1-cre+ littermate were injected 3 times with polyIC (20mcg/g of body weight) every other day. Mice were sacrificed and analyzed 2 weeks after the last injection. LSK cells were sorted.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL1261
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686728/suppl/GSM686728.CEL.gz
| Sample_series_id | GSE27799
| Sample_series_id | GSE27833
| Sample_data_row_count | 45101
| |
|
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