Search results for the GEO ID: GSE27973  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM692115 | GPL570 |
|
Donor 1 - medium
|
Human bronchial epithelial cells - 24h medium
|
donor: 1
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: medium
|
Gene expression in response to medium
Sample 1A
EA09124_102512_H133+_1A.CHP
EA09124_102512_H133+_1A.CEL
|
| Sample_geo_accession | GSM692115
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692115/suppl/GSM692115.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692115/suppl/GSM692115.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692116 | GPL570 |
|
Donor 1 - RV16
|
Human bronchial epithelial cells - 24 h RV16
|
donor: 1
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16
|
Gene expression in response to RV16 infection
Sample 1B
EA09124_102513_H133+_1B.CHP
EA09124_102513_H133+_1B.CEL
|
| Sample_geo_accession | GSM692116
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692116/suppl/GSM692116.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692116/suppl/GSM692116.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692117 | GPL570 |
|
Donor 1 - CSE
|
Human bronchial epithelial cells - 24 h CSE
|
donor: 1
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
|
Gene expression in response to CSE exposure
Sample 1C
EA09124_102514_H133+_1C.CHP
EA09124_102514_H133+_1C.CEL
|
| Sample_geo_accession | GSM692117
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692117/suppl/GSM692117.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692117/suppl/GSM692117.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692118 | GPL570 |
|
Donor 1 - RV16+CSE
|
Human bronchial epithelial cells - 24 h RV16+CSE
|
donor: 1
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16+CSE
|
Gene Expression in response to RV16 in the presence of CSE
Sample 1D
EA09124_102515_H133+_1D.CHP
EA09124_102515_H133+_1D.CEL
|
| Sample_geo_accession | GSM692118
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692118/suppl/GSM692118.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692118/suppl/GSM692118.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692119 | GPL570 |
|
Donor 2 - medium
|
Human bronchial epithelial cells - 24h medium
|
donor: 2
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: medium
|
Gene expression in response to medium
Sample 2A
EA09124_102516_H133+_2A.CHP
EA09124_102516_H133+_2A.CEL
|
| Sample_geo_accession | GSM692119
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692119/suppl/GSM692119.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692119/suppl/GSM692119.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692120 | GPL570 |
|
Donor 2 - RV16
|
Human bronchial epithelial cells - 24 h RV16
|
donor: 2
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16
|
Gene expression in response to RV16 infection
Sample 2B
EA09124_102517_H133+_2B.CHP
EA09124_102517_H133+_2B.CEL
|
| Sample_geo_accession | GSM692120
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692120/suppl/GSM692120.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692120/suppl/GSM692120.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692121 | GPL570 |
|
Donor 2 - CSE
|
Human bronchial epithelial cells - 24 h CSE
|
donor: 2
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
|
Gene expression in response to CSE exposure
Sample 2C
EA09124_102518_H133+_2C.CHP
EA09124_102518_H133+_2C.CEL
|
| Sample_geo_accession | GSM692121
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692121/suppl/GSM692121.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692121/suppl/GSM692121.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692122 | GPL570 |
|
Donor 2 - RV16+CSE
|
Human bronchial epithelial cells - 24 h RV16+CSE
|
donor: 2
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16+CSE
|
Gene Expression in response to RV16 in the presence of CSE
Sample 2D
EA09124_102519_H133+_2D.CHP
EA09124_102519_H133+_2D.CEL
|
| Sample_geo_accession | GSM692122
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692122/suppl/GSM692122.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692122/suppl/GSM692122.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692123 | GPL570 |
|
Donor 3 - medium
|
Human bronchial epithelial cells - 24h medium
|
donor: 3
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: medium
|
Gene expression in response to medium
Sample 3A
EA09124_102520_H133+_3A.CHP
EA09124_102520_H133+_3A.CEL
|
| Sample_geo_accession | GSM692123
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692123/suppl/GSM692123.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692123/suppl/GSM692123.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692124 | GPL570 |
|
Donor 3 - RV16
|
Human bronchial epithelial cells - 24 h RV16
|
donor: 3
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16
|
Gene expression in response to RV16 infection
Sample 3B
EA09124_102521_H133+_3B.CHP
EA09124_102521_H133+_3B.CEL
|
| Sample_geo_accession | GSM692124
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692124/suppl/GSM692124.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692124/suppl/GSM692124.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692125 | GPL570 |
|
Donor 3 - CSE
|
Human bronchial epithelial cells - 24 h CSE
|
donor: 3
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
|
Gene expression in response to CSE exposure
Sample 3C
EA09124_102522_H133+_3C.CHP
EA09124_102522_H133+_3C.CEL
|
| Sample_geo_accession | GSM692125
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692125/suppl/GSM692125.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692125/suppl/GSM692125.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692126 | GPL570 |
|
Donor 3 - RV16+CSE
|
Human bronchial epithelial cells - 24 h RV16+CSE
|
donor: 3
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16+CSE
|
Gene Expression in response to RV16 in the presence of CSE
Sample 3D
EA09124_102523_H133+_3D.CHP
EA09124_102523_H133+_3D.CEL
|
| Sample_geo_accession | GSM692126
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692126/suppl/GSM692126.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692126/suppl/GSM692126.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692127 | GPL570 |
|
Donor 4 - medium
|
Human bronchial epithelial cells - 24h medium
|
donor: 4
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: medium
|
Gene expression in response to medium
Sample 4A
EA09124_102524_H133+_4A.CHP
EA09124_102524_H133+_4A.CEL
|
| Sample_geo_accession | GSM692127
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692127/suppl/GSM692127.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692127/suppl/GSM692127.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692128 | GPL570 |
|
Donor 4 - RV16
|
Human bronchial epithelial cells - 24 h RV16
|
donor: 4
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16
|
Gene expression in response to RV16 infection
Sample 4B
EA09124_102525_H133+_4B.CHP
EA09124_102525_H133+_4B.CEL
|
| Sample_geo_accession | GSM692128
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692128/suppl/GSM692128.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692128/suppl/GSM692128.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692129 | GPL570 |
|
Donor 4 - CSE
|
Human bronchial epithelial cells - 24 h CSE
|
donor: 4
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
|
Gene expression in response to CSE exposure
Sample 4C
EA09124_102526_H133+_4C.CHP
EA09124_102526_H133+_4C.CEL
|
| Sample_geo_accession | GSM692129
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692129/suppl/GSM692129.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692129/suppl/GSM692129.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
|
GSM692130 | GPL570 |
|
Donor 4 - RV16+CSE
|
Human bronchial epithelial cells - 24 h RV16+CSE
|
donor: 4
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: RV16+CSE
|
Gene Expression in response to RV16 in the presence of CSE
Sample 4D
EA09124_102527_H133+_4D.CHP
EA09124_102527_H133+_4D.CEL
|
| Sample_geo_accession | GSM692130
| | Sample_status | Public on Mar 15 2011
| | Sample_submission_date | Mar 15 2011
| | Sample_last_update_date | Mar 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI | 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
| | Sample_growth_protocol_ch1 | Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
| | Sample_hyb_protocol | Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Proud
| | Sample_contact_email | dproud@ucalgary.ca
| | Sample_contact_laboratory | HRIC 4AC60
| | Sample_contact_department | Physiology & Pharmacology
| | Sample_contact_institute | University of Calgary
| | Sample_contact_address | 3330 Hospital DRive NW
| | Sample_contact_city | Calgary
| | Sample_contact_state | AB
| | Sample_contact_zip/postal_code | T2N 4N1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692130/suppl/GSM692130.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692130/suppl/GSM692130.CHP.gz
| | Sample_series_id | GSE27973
| | Sample_data_row_count | 54675
| | |
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