Result

Search results for the GEO ID: GSE27999

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Title

Source name

Description

Characteristics

GSM692646

GPL1261

E15 control kidney pull 1

pull of 16 mouse kidneys at the developmental stage E15 under normal diet

diet: normal diet tissue: Kidney developmental stage: E15 strain: C57BL/6 gender: female

gene expression data from pull of 16 mouse kidneys at the developmental stage E15 under normal diet

Sample_geo_accession	GSM692646
Sample_status	Public on Mar 15 2012
Sample_submission_date	Mar 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Female mice C57BL/6 (Charles River, USA) were used in the experiments. 8-weeks-old female mice were divided into two groups, control and iron deficient. The iron deficient group was maintained on an iron deficient synthetic diet containing approximately 2-6ppm iron (TD.80396, Harlan,USA). The control group was provided with an iron sufficient diet containing 220ppm iron. Water was served ad libitum in plastic bottles. At least 20 mice were included in each group. Both experimental and control groups were fed the diets for 3 weeks prior to gestation. After one month the females were mated with C57BL/6 males. The following morning, females were checked for the presence of vaginal plugs. If a plug was present, that morning was designated as E0.5. Females were fed their designated diets during the breeding period and throughout pregnancy
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	total RNA was extracted using RNAqueous Micro Kit (Ambion) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	biotinylated cRNA was prepared using Encore Biotin Module (Nugen) according to the manufacturer's instructions.
Sample_hyb_protocol	Following fragmentation, 15 ug of fragmented and labeled cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrics GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with robust multichip analysis (RMA) using the Affymetrix microarray *.cel image file and GeneTraffic(R) (Silicon Genetics) software
Sample_platform_id	GPL1261
Sample_contact_name	Max,,Werth
Sample_contact_institute	Columbia University
Sample_contact_address	630WEST 168th STREET P&S 10-501
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692646/suppl/GSM692646.CEL.gz
Sample_series_id	GSE27999
Sample_data_row_count	45101

GSM692647

GPL1261

E15 control kidney pull 2

pull of 16 mouse kidneys at the developmental stage E15 under normal diet

diet: normal diet tissue: Kidney developmental stage: E15 strain: C57BL/6 gender: female

gene expression data from pull of 16 mouse kidneys at the developmental stage E15 under normal diet

Sample_geo_accession	GSM692647
Sample_status	Public on Mar 15 2012
Sample_submission_date	Mar 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Female mice C57BL/6 (Charles River, USA) were used in the experiments. 8-weeks-old female mice were divided into two groups, control and iron deficient. The iron deficient group was maintained on an iron deficient synthetic diet containing approximately 2-6ppm iron (TD.80396, Harlan,USA). The control group was provided with an iron sufficient diet containing 220ppm iron. Water was served ad libitum in plastic bottles. At least 20 mice were included in each group. Both experimental and control groups were fed the diets for 3 weeks prior to gestation. After one month the females were mated with C57BL/6 males. The following morning, females were checked for the presence of vaginal plugs. If a plug was present, that morning was designated as E0.5. Females were fed their designated diets during the breeding period and throughout pregnancy
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	total RNA was extracted using RNAqueous Micro Kit (Ambion) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	biotinylated cRNA was prepared using Encore Biotin Module (Nugen) according to the manufacturer's instructions.
Sample_hyb_protocol	Following fragmentation, 15 ug of fragmented and labeled cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrics GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with robust multichip analysis (RMA) using the Affymetrix microarray *.cel image file and GeneTraffic(R) (Silicon Genetics) software
Sample_platform_id	GPL1261
Sample_contact_name	Max,,Werth
Sample_contact_institute	Columbia University
Sample_contact_address	630WEST 168th STREET P&S 10-501
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692647/suppl/GSM692647.CEL.gz
Sample_series_id	GSE27999
Sample_data_row_count	45101

GSM692648

GPL1261

E15 iron deficient kidney pull 1

pull of 16 mouse kidneys at the developmental stage E15 under iron deficient diet

diet: iron deficient diet tissue: Kidney developmental stage: E15 strain: C57BL/6 gender: female

gene expression data from pull of 16 mouse kidneys at the developmental stage E15 under iron defecient diet

Sample_geo_accession	GSM692648
Sample_status	Public on Mar 15 2012
Sample_submission_date	Mar 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Female mice C57BL/6 (Charles River, USA) were used in the experiments. 8-weeks-old female mice were divided into two groups, control and iron deficient. The iron deficient group was maintained on an iron deficient synthetic diet containing approximately 2-6ppm iron (TD.80396, Harlan,USA). The control group was provided with an iron sufficient diet containing 220ppm iron. Water was served ad libitum in plastic bottles. At least 20 mice were included in each group. Both experimental and control groups were fed the diets for 3 weeks prior to gestation. After one month the females were mated with C57BL/6 males. The following morning, females were checked for the presence of vaginal plugs. If a plug was present, that morning was designated as E0.5. Females were fed their designated diets during the breeding period and throughout pregnancy
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	total RNA was extracted using RNAqueous Micro Kit (Ambion) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	biotinylated cRNA was prepared using Encore Biotin Module (Nugen) according to the manufacturer's instructions.
Sample_hyb_protocol	Following fragmentation, 15 ug of fragmented and labeled cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrics GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with robust multichip analysis (RMA) using the Affymetrix microarray *.cel image file and GeneTraffic(R) (Silicon Genetics) software
Sample_platform_id	GPL1261
Sample_contact_name	Max,,Werth
Sample_contact_institute	Columbia University
Sample_contact_address	630WEST 168th STREET P&S 10-501
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692648/suppl/GSM692648.CEL.gz
Sample_series_id	GSE27999
Sample_data_row_count	45101

GSM692649

GPL1261

E15 iron deficient kidney pull 2

pull of 16 mouse kidneys at the developmental stage E15 under iron deficient diet

diet: iron deficient diet tissue: Kidney developmental stage: E15 strain: C57BL/6 gender: female

gene expression data from pull of 16 mouse kidneys at the developmental stage E15 under iron defecient diet

Sample_geo_accession	GSM692649
Sample_status	Public on Mar 15 2012
Sample_submission_date	Mar 16 2011
Sample_last_update_date	Mar 15 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Female mice C57BL/6 (Charles River, USA) were used in the experiments. 8-weeks-old female mice were divided into two groups, control and iron deficient. The iron deficient group was maintained on an iron deficient synthetic diet containing approximately 2-6ppm iron (TD.80396, Harlan,USA). The control group was provided with an iron sufficient diet containing 220ppm iron. Water was served ad libitum in plastic bottles. At least 20 mice were included in each group. Both experimental and control groups were fed the diets for 3 weeks prior to gestation. After one month the females were mated with C57BL/6 males. The following morning, females were checked for the presence of vaginal plugs. If a plug was present, that morning was designated as E0.5. Females were fed their designated diets during the breeding period and throughout pregnancy
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	total RNA was extracted using RNAqueous Micro Kit (Ambion) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	biotinylated cRNA was prepared using Encore Biotin Module (Nugen) according to the manufacturer's instructions.
Sample_hyb_protocol	Following fragmentation, 15 ug of fragmented and labeled cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrics GeneChip Scanner 3000
Sample_data_processing	The data were analyzed with robust multichip analysis (RMA) using the Affymetrix microarray *.cel image file and GeneTraffic(R) (Silicon Genetics) software
Sample_platform_id	GPL1261
Sample_contact_name	Max,,Werth
Sample_contact_institute	Columbia University
Sample_contact_address	630WEST 168th STREET P&S 10-501
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM692nnn/GSM692649/suppl/GSM692649.CEL.gz
Sample_series_id	GSE27999
Sample_data_row_count	45101

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