Result

Search results for the GEO ID: GSE28031

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Description

Characteristics

GSM693652

GPL1261

Failing heart_apoE-deficient_2 months of rosiglitazone_age 8 months_rep1

Failing heart, apoE-deficient, 2 months of rosiglitazone, age 8 months

strain/background: B6 genotype: apoE-deficient age: 8 months tissue: failing heart treatment: 2 months of rosiglitazone

Apoe-rosiglitazone-1 Gene expression data of failing apoE-deficient heart with 2 months of rosiglitazone, age 8 months.

Sample_geo_accession	GSM693652
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693652/suppl/GSM693652.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693652/suppl/GSM693652.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

GSM693653

GPL1261

Failing heart_apoE-deficient_2 months of rosiglitazone_age 8 months_rep2

Failing heart, apoE-deficient, 2 months of rosiglitazone, age 8 months

strain/background: B6 genotype: apoE-deficient age: 8 months tissue: failing heart treatment: 2 months of rosiglitazone

Apoe-rosiglitazone-2 Gene expression data of failing apoE-deficient heart with 2 months of rosiglitazone, age 8 months.

Sample_geo_accession	GSM693653
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693653/suppl/GSM693653.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693653/suppl/GSM693653.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

GSM693654

GPL1261

Heart_apoE-deficient_age 8 months_rep1

Heart, apoE-deficient, untreated, age 8 months

strain/background: B6 genotype: apoE-deficient age: 8 months tissue: heart treatment: none

Apoe-8mo-1 Gene expression data of apoE-deficient heart, age 8 months.

Sample_geo_accession	GSM693654
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693654/suppl/GSM693654.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693654/suppl/GSM693654.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

GSM693655

GPL1261

Heart_apoE-deficient_age 8 months_rep2

Heart, apoE-deficient, untreated, age 8 months

strain/background: B6 genotype: apoE-deficient age: 8 months tissue: heart treatment: none

Apoe-8mo-2 Gene expression data of apoE-deficient heart, age 8 months.

Sample_geo_accession	GSM693655
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693655/suppl/GSM693655.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693655/suppl/GSM693655.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

GSM693656

GPL1261

Control heart_age 8 months_BSIX_rep1

Control heart, B6, untreated, age 8 months

strain/background: B6 genotype: wild type age: 8 months tissue: control heart treatment: none

BSIX-8mo-1 Gene expression data of control B6 heart, age 8 months.

Sample_geo_accession	GSM693656
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693656/suppl/GSM693656.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693656/suppl/GSM693656.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

GSM693657

GPL1261

Control heart_age 8 months_BSIX_rep2

Control heart, B6, untreated, age 8 months

strain/background: B6 genotype: wild type age: 8 months tissue: control heart treatment: none

BSIX-8mo-2 Gene expression data of control B6 heart, age 8 months.

Sample_geo_accession	GSM693657
Sample_status	Public on Mar 18 2011
Sample_submission_date	Mar 17 2011
Sample_last_update_date	Mar 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) failing hearts from 8-month-old apoE-deficient mice with 2 months of rosiglitazone treatment (30 mg/kg/d), (ii) hearts from 8-month-old untreated apoE-deficient mice, and (iii) hearts from 8-month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 8-month-old apolipoprotein E (apoE)-deficient mice treated for 2 months with the anti-diabetic drug rosiglitazone. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 6 months, the treatment group of apoE-deficient mice received rosiglitazone (30 mg/kg/d) for 2 months. At an age of 8 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693657/suppl/GSM693657.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM693nnn/GSM693657/suppl/GSM693657.CHP.gz
Sample_series_id	GSE28031
Sample_data_row_count	45101

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