Search results for the GEO ID: GSE28117  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM696436 | GPL570 |
|
HUVEC_IL-4,0h
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HUVEC, IL-4 0h
|
cell: HUVEC
stimulation: IL-4 treatment for 0h
|
|
| Sample_geo_accession | GSM696436
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 23 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM696nnn/GSM696436/suppl/GSM696436.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM699979 | GPL570 |
|
HUVEC_IL-4,1h
|
HUVEC, IL-4 1h
|
cell: HUVEC
stimulation: IL-4 treatment for 1h
|
|
| Sample_geo_accession | GSM699979
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 31 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699979/suppl/GSM699979.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM699980 | GPL570 |
|
HUVEC_IL-4,2h
|
HUVEC, IL-4 2h
|
cell: HUVEC
stimulation: IL-4 treatment for 2h
|
|
| Sample_geo_accession | GSM699980
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 31 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699980/suppl/GSM699980.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM699981 | GPL570 |
|
HUVEC_IL-4,4h
|
HUVEC, IL-4 4h
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cell: HUVEC
stimulation: IL-4 treatment for 4h
|
|
| Sample_geo_accession | GSM699981
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 31 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699981/suppl/GSM699981.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM699982 | GPL570 |
|
HUVEC_IL-4,8h
|
HUVEC, IL-4 8h
|
cell: HUVEC
stimulation: IL-4 treatment for 8h
|
|
| Sample_geo_accession | GSM699982
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 31 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699982/suppl/GSM699982.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM699983 | GPL570 |
|
HUVEC_IL-4,16h
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HUVEC, IL-4 16h
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cell: HUVEC
stimulation: IL-4 treatment for 16h
|
|
| Sample_geo_accession | GSM699983
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Mar 31 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699983/suppl/GSM699983.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM703089 | GPL570 |
|
HUVEC_si-control IL-4_0h
|
HUVEC, IL-4 0h, si-control #1
|
cell: HUVEC
stimulation: IL-4 treatment for 0h
treatment: si-control #1
|
|
| Sample_geo_accession | GSM703089
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 07 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM703nnn/GSM703089/suppl/GSM703089.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM707972 | GPL570 |
|
HUVEC_si-control IL-4_4h
|
HUVEC, IL-4 4h, si-control #1
|
cell: HUVEC
stimulation: IL-4 treatment for 4h
treatment: si-control #1
|
|
| Sample_geo_accession | GSM707972
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 13 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707972/suppl/GSM707972.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM707973 | GPL570 |
|
HUVEC_si-STAT6 Oligo1,IL-4_4h
|
HUVEC, IL-4 4h, si-STAT6 Oligo1
|
cell: HUVEC
stimulation: IL-4 treatment for 4h
treatment: si-STAT6 Oligo1
|
|
| Sample_geo_accession | GSM707973
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 14 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707973/suppl/GSM707973.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM707974 | GPL570 |
|
HUVEC_si-STAT6 Oligo2,IL-4_4h
|
HUVEC, IL-4 4h, si-STAT6 Oligo2
|
cell: HUVEC
stimulation: IL-4 treatment for 4h
treatment: si-STAT6 Oligo2
|
|
| Sample_geo_accession | GSM707974
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 14 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707974/suppl/GSM707974.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM707975 | GPL570 |
|
HUVEC_control-Adenovirus
|
HUVEC, Control-Adenovirus 48h
|
cell: HUVEC
infection: Control-Adenovirus for 48h
treatment: Ad-Ctrl
|
|
| Sample_geo_accession | GSM707975
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 14 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707975/suppl/GSM707975.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
|
GSM707976 | GPL570 |
|
HUVEC_CA-STAT6-Ad #1
|
HUVEC, CA-STAT6-Adenovirus 48h #1
|
cell: HUVEC
infection: Constitutive Active STAT6 Adenovirus for 48h #1
treatment: Ad-CA-STAT6 #1
|
|
| Sample_geo_accession | GSM707976
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 14 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707976/suppl/GSM707976.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
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GSM707977 | GPL570 |
|
HUVEC_CA-STAT6-Ad #2
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HUVEC, CA-STAT6-Adenovirus 48h #2
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cell: HUVEC
infection: Constitutive Active STAT6 Adenovirus for 48h #2
treatment: Ad-CA-STAT6 #2
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|
| Sample_geo_accession | GSM707977
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Apr 14 2011
| | Sample_last_update_date | Jun 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen). 2 micrograms of total RNA was reverse transcribed using the SuperScript II system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| | Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| | Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| | Sample_data_processing | Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yasuharu,,Kanki
| | Sample_contact_email | kanki@lsbm.org
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 4-6-1 Komaba, Meguro-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 153-8904
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707977/suppl/GSM707977.CEL.gz
| | Sample_series_id | GSE28117
| | Sample_data_row_count | 54675
| | |
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