Search results for the GEO ID: GSE28167 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM697600 | GPL570 |
|
U251 aligned 1
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on highly-aligned electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697600
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697600/suppl/GSM697600_01_A48-1.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
GSM697601 | GPL570 |
|
U251 aligned 2
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on highly-aligned electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697601
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697601/suppl/GSM697601_05_A48-2.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
GSM697602 | GPL570 |
|
U251 aligned 3
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on highly-aligned electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697602
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697602/suppl/GSM697602_06_A72-2.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
GSM697603 | GPL570 |
|
U251 random 1
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on randomly-oriented electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697603
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697603/suppl/GSM697603_03_R48-1.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
GSM697604 | GPL570 |
|
U251 random 2
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on randomly-oriented electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697604
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697604/suppl/GSM697604_07_R48-2.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
GSM697605 | GPL570 |
|
U251 random 3
|
human glioma cells
|
cell line: Brain U251MG glioma cells
treatment: cultured on randomly-oriented electrospun nanofibers of poly-caprolactone
|
|
Sample_geo_accession | GSM697605
| Sample_status | Public on Mar 25 2011
| Sample_submission_date | Mar 24 2011
| Sample_last_update_date | Mar 25 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were plated on nanofibers and allowed to grow and migrate without further treatments for 24h until collection.
| Sample_growth_protocol_ch1 | Cells (5x10E5) were dissociated and cultured on nanofiber-coated 60mm-dishes for 24h before RNA collection. Culture medium was DMEM with 10% fetal bovine serum and standard antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated from cells cultured on nanofibers using Trizol (Invitrogen) and RNAeasy kit (Qiagen), according to manufacturers' instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10ug total RNA, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, 2001)
| Sample_hyb_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_scan_protocol | Standard Affymetrix procedures performed at the Ohio State University Microarray Shared Resource (http://www.osuccc.osu.edu/microarray)
| Sample_data_processing | The data were analyzed with Robust Multichip Average (RMA) procedure using quantile normalization .
| Sample_platform_id | GPL570
| Sample_contact_name | Mariano,Sebastian,Viapiano
| Sample_contact_email | viapiano.1@osu.edu
| Sample_contact_phone | 614-292-4362
| Sample_contact_fax | 614-292-5379
| Sample_contact_department | Neurological Surgery
| Sample_contact_institute | The Ohio State University
| Sample_contact_address | 1060 Carmack Rd
| Sample_contact_city | Columbus
| Sample_contact_state | OH
| Sample_contact_zip/postal_code | 43210
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.viapianolab.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM697nnn/GSM697605/suppl/GSM697605_08_R72-2.CEL.gz
| Sample_series_id | GSE28167
| Sample_data_row_count | 54675
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
|
Filter results by number of probes |
|
|