Search results for the GEO ID: GSE28277 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM699798 | GPL1261 |
|
Control 1
|
mouse placenta including decidua, control
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: control
|
FVB C1
|
Sample_geo_accession | GSM699798
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699798/suppl/GSM699798.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699798/suppl/GSM699798.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699799 | GPL1261 |
|
Control 2
|
mouse placenta including decidua, control
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: control
|
FVB C2
|
Sample_geo_accession | GSM699799
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699799/suppl/GSM699799.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699799/suppl/GSM699799.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699800 | GPL1261 |
|
Control 3
|
mouse placenta including decidua, control
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: control
|
FVB C3
|
Sample_geo_accession | GSM699800
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699800/suppl/GSM699800.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699800/suppl/GSM699800.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699801 | GPL1261 |
|
Control 4
|
mouse placenta including decidua, control
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: control
|
FVB C4
|
Sample_geo_accession | GSM699801
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699801/suppl/GSM699801.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699801/suppl/GSM699801.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699802 | GPL1261 |
|
Control 5
|
mouse placenta including decidua, control
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: control
|
FVB C5
|
Sample_geo_accession | GSM699802
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699802/suppl/GSM699802.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699802/suppl/GSM699802.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699803 | GPL1261 |
|
Experimental 1
|
mouse placenta including decidua, diabetic
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
AE1 Diab
|
Sample_geo_accession | GSM699803
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699803/suppl/GSM699803.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699803/suppl/GSM699803.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699804 | GPL1261 |
|
Experimental 2
|
mouse placenta including decidua, diabetic
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
AE2 Diab
|
Sample_geo_accession | GSM699804
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699804/suppl/GSM699804.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699804/suppl/GSM699804.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699805 | GPL1261 |
|
Experimental 3
|
mouse placenta including decidua, diabetic
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
AE3 Diab
|
Sample_geo_accession | GSM699805
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699805/suppl/GSM699805.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699805/suppl/GSM699805.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699806 | GPL1261 |
|
Experimental 4
|
mouse placenta including decidua, diabetic
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
AE4 Diab
|
Sample_geo_accession | GSM699806
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699806/suppl/GSM699806.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699806/suppl/GSM699806.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
GSM699807 | GPL1261 |
|
Experimental 5
|
mouse placenta including decidua, diabetic
|
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
AE5 Diab
|
Sample_geo_accession | GSM699807
| Sample_status | Public on Jun 01 2011
| Sample_submission_date | Mar 30 2011
| Sample_last_update_date | Jun 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
| Sample_hyb_protocol | GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
| Sample_platform_id | GPL1261
| Sample_contact_name | Claudia,,Kappen
| Sample_contact_email | claudia.kappen@pbrc.edu
| Sample_contact_phone | 225-763-2781
| Sample_contact_department | Developmental Biology
| Sample_contact_institute | Pennington Biomedical Research Center
| Sample_contact_address | 6400 Perkins Road
| Sample_contact_city | Baton Rouge
| Sample_contact_state | LA
| Sample_contact_zip/postal_code | 70808
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699807/suppl/GSM699807.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM699nnn/GSM699807/suppl/GSM699807.CHP.gz
| Sample_series_id | GSE28277
| Sample_data_row_count | 45101
| |
|
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