Search results for the GEO ID: GSE28317 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM700450 | GPL570 |
|
AML1-ETO CD34+ cells rep1
|
CD34+ cells infected with MIGR1-AML1-ETO
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO
|
|
Sample_geo_accession | GSM700450
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700450/suppl/GSM700450_LW1_AML1-ETO-1_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700450/suppl/GSM700450_LW1_AML1-ETO-1_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700451 | GPL570 |
|
AML1-ETO CD34+ cells rep2
|
CD34+ cells infected with MIGR1-AML1-ETO
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO
|
|
Sample_geo_accession | GSM700451
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700451/suppl/GSM700451_LW2_AML1-ETO-2_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700451/suppl/GSM700451_LW2_AML1-ETO-2_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700452 | GPL570 |
|
AML1-ETO CD34+ cells rep3
|
CD34+ cells infected with MIGR1-AML1-ETO
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO
|
|
Sample_geo_accession | GSM700452
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700452/suppl/GSM700452_LW3_AML1-ETO-3_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700452/suppl/GSM700452_LW3_AML1-ETO-3_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700453 | GPL570 |
|
AML1-ETO∆NHR1 CD34+ cells rep1
|
CD34+ cells infected with MIGR1-AML1-ETO∆NHR1
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO∆NHR1
|
|
Sample_geo_accession | GSM700453
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700453/suppl/GSM700453_LW4_AML1-ETOdelNHR1-1_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700453/suppl/GSM700453_LW4_AML1-ETOdelNHR1-1_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700454 | GPL570 |
|
AML1-ETO∆NHR1 CD34+ cells rep2
|
CD34+ cells infected with MIGR1-AML1-ETO∆NHR1
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO∆NHR1
|
|
Sample_geo_accession | GSM700454
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700454/suppl/GSM700454_LW5_AML1-ETOdelNHR1-2_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700454/suppl/GSM700454_LW5_AML1-ETOdelNHR1-2_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700455 | GPL570 |
|
AML1-ETO∆NHR1 CD34+ cells rep3
|
CD34+ cells infected with MIGR1-AML1-ETO∆NHR1
|
cell type: CD34+ cord blood cells
infection: MIGR1-AML1-ETO∆NHR1
|
|
Sample_geo_accession | GSM700455
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700455/suppl/GSM700455_LW6_AML1-ETOdelNHR1-3_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700455/suppl/GSM700455_LW6_AML1-ETOdelNHR1-3_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700456 | GPL570 |
|
MIGR1 CD34+ cells rep1
|
CD34+ cells infected with MIGR1
|
cell type: CD34+ cord blood cells
infection: MIGR1
|
|
Sample_geo_accession | GSM700456
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700456/suppl/GSM700456_LW7_MIGR1-1_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700456/suppl/GSM700456_LW7_MIGR1-1_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700457 | GPL570 |
|
MIGR1 CD34+ cells rep2
|
CD34+ cells infected with MIGR1
|
cell type: CD34+ cord blood cells
infection: MIGR1
|
|
Sample_geo_accession | GSM700457
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700457/suppl/GSM700457_LW8_MIGR1-2_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700457/suppl/GSM700457_LW8_MIGR1-2_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
|
GSM700458 | GPL570 |
|
MIGR1 CD34+ cells rep3
|
CD34+ cells infected with MIGR1
|
cell type: CD34+ cord blood cells
infection: MIGR1
|
|
Sample_geo_accession | GSM700458
| Sample_status | Public on Jul 19 2011
| Sample_submission_date | Apr 01 2011
| Sample_last_update_date | Jul 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Human CD34+ cord blood cells were infected with retrovirus expressing MIGR1, AML1-ETO and AML1-ETO∆NHR1. CD34+GFP+ cells were isolated by FACS.
| Sample_growth_protocol_ch1 | 20% BIT IMEM media with human SCF, TPO, Flt3L and IL6, 37 degree 5% CO2 incubator
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction plus kit was used to isolate RNA.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
| Sample_hyb_protocol | 10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
| Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| Sample_data_processing | Feature intensity was extracted by Affymetrix GeneChip Command Console as CEL files. Chp fies are generated with Affymetrix expression console using MAS5.0.
| Sample_platform_id | GPL570
| Sample_contact_name | Jeffrey,,Zhao
| Sample_contact_email | zhaoy@mskcc.org
| Sample_contact_department | Genomics Core
| Sample_contact_institute | Memorial Sloan Kettering Cancer Center
| Sample_contact_address | 1275 York Avenue
| Sample_contact_city | New York
| Sample_contact_state | RRL417A
| Sample_contact_zip/postal_code | 10065
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700458/suppl/GSM700458_LW9_MIGR1-3_HG-U133_Plus_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700458/suppl/GSM700458_LW9_MIGR1-3_HG-U133_Plus_2.mas5.CHP.gz
| Sample_series_id | GSE28317
| Sample_data_row_count | 54675
| |
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