Search results for the GEO ID: GSE28342  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM700782 | GPL1261 |
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DN3 KO5
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Lin neg., CD44 neg., CD25 pos. thymocytes
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cell type: Lin neg., CD44 neg., CD25 pos. thymocytes
genotype: Miz1 knockout
genetic background: C57BL/6
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Miz1 knockout
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| Sample_geo_accession | GSM700782
| | Sample_status | Public on Aug 24 2011
| | Sample_submission_date | Apr 02 2011
| | Sample_last_update_date | Aug 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol (GIBCOBRL, Life Technologies) was used to prepare total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200ng of target RNA was reverse transcribed into cDNA using the Ambion MessageAMP II Biotin Enhanced Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MG-430_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (G7 update).
| | Sample_data_processing | The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lothar,,Vassen
| | Sample_contact_email | lothar.vassen@ircm.qc.ca
| | Sample_contact_phone | 514-987-3351
| | Sample_contact_laboratory | Dr. Möröy
| | Sample_contact_department | Hematopoiesis and Cancer
| | Sample_contact_institute | IRCM
| | Sample_contact_address | 110, avenue des Pins Ouest
| | Sample_contact_city | Montréal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H2W 1R7
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700782/suppl/GSM700782_DN3_KO5.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700782/suppl/GSM700782_DN3_KO5.CHP.gz
| | Sample_series_id | GSE28342
| | Sample_data_row_count | 45101
| | |
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GSM700783 | GPL1261 |
|
DN3 WT2
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Lin neg., CD44 neg., CD25 pos. thymocytes
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cell type: Lin neg., CD44 neg., CD25 pos. thymocytes
genotype: wt
genetic background: C57BL/6
|
wt
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| Sample_geo_accession | GSM700783
| | Sample_status | Public on Aug 24 2011
| | Sample_submission_date | Apr 02 2011
| | Sample_last_update_date | Aug 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol (GIBCOBRL, Life Technologies) was used to prepare total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200ng of target RNA was reverse transcribed into cDNA using the Ambion MessageAMP II Biotin Enhanced Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MG-430_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (G7 update).
| | Sample_data_processing | The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lothar,,Vassen
| | Sample_contact_email | lothar.vassen@ircm.qc.ca
| | Sample_contact_phone | 514-987-3351
| | Sample_contact_laboratory | Dr. Möröy
| | Sample_contact_department | Hematopoiesis and Cancer
| | Sample_contact_institute | IRCM
| | Sample_contact_address | 110, avenue des Pins Ouest
| | Sample_contact_city | Montréal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H2W 1R7
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700783/suppl/GSM700783_DN3_WT2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM700nnn/GSM700783/suppl/GSM700783_DN3_WT2.CHP.gz
| | Sample_series_id | GSE28342
| | Sample_data_row_count | 45101
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