Search results for the GEO ID: GSE28414 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM702226 | GPL570 |
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MCF7VP cell line
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MCF7VP_siControl
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cell line: MCF7VP
tissue: breast
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Gene expression data from etoposide resistant breast cancer cell line
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Sample_geo_accession | GSM702226
| Sample_status | Public on Apr 08 2011
| Sample_submission_date | Apr 06 2011
| Sample_last_update_date | Apr 08 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cell lines were cultured for at least 8 passages in 4µM etoposide in IMEM with 10% FBS and 1% Pen-Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated from the cells using the mirVana kit from ABI as per manufacturers instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100 ng of total RNA were amplified and labeled using the message amp II-biotin enhanced reagents (Ambion) according to the protocol provided by the supplier
| Sample_hyb_protocol | 11 µg of labeled cRNA, washed, stained, and scanned according to the protocol described in Affymetrix GeneChip Expression Analysis Manual (Fluidics protocol FS450_0001)
| Sample_scan_protocol | Affymetrix GeneChip scanner 7G
| Sample_data_processing | The data were analyzed using Partek Genomic Suite with robust mutlichip averaging (RMA) as the normalization method. Normalized intensities less than 3 were set to 3.
| Sample_platform_id | GPL570
| Sample_contact_name | Kate,McGee,Im
| Sample_contact_institute | National Cancer Institute at Frederick
| Sample_contact_address | 1050 Boyles Street
| Sample_contact_city | Frederick
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21702
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM702nnn/GSM702226/suppl/GSM702226_MCF7VP_siControl.CEL.gz
| Sample_series_id | GSE28414
| Sample_series_id | GSE28415
| Sample_data_row_count | 54675
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GSM702227 | GPL570 |
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MCF7VP cell line, RUNX2 knockdown
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MCF7VP_siRUNX2
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cell line: MCF7VP
tissue: breast
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Gene expression data from etoposide resistant breast cancer cell line
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Sample_geo_accession | GSM702227
| Sample_status | Public on Apr 08 2011
| Sample_submission_date | Apr 06 2011
| Sample_last_update_date | Apr 08 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Cell lines were cultured for at least 8 passages in 4µM etoposide in IMEM with 10% FBS and 1% Pen-Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated from the cells using the mirVana kit from ABI as per manufacturers instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100 ng of total RNA were amplified and labeled using the message amp II-biotin enhanced reagents (Ambion) according to the protocol provided by the supplier
| Sample_hyb_protocol | 11 µg of labeled cRNA, washed, stained, and scanned according to the protocol described in Affymetrix GeneChip Expression Analysis Manual (Fluidics protocol FS450_0001)
| Sample_scan_protocol | Affymetrix GeneChip scanner 7G
| Sample_data_processing | The data were analyzed using Partek Genomic Suite with robust mutlichip averaging (RMA) as the normalization method. Normalized intensities less than 3 were set to 3.
| Sample_platform_id | GPL570
| Sample_contact_name | Kate,McGee,Im
| Sample_contact_institute | National Cancer Institute at Frederick
| Sample_contact_address | 1050 Boyles Street
| Sample_contact_city | Frederick
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21702
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM702nnn/GSM702227/suppl/GSM702227_MCF7VP_siRUNX2.CEL.gz
| Sample_series_id | GSE28414
| Sample_series_id | GSE28415
| Sample_data_row_count | 54675
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