Search results for the GEO ID: GSE28502 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM706153 | GPL570 |
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SMC-Control-T
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HUVSMC cell line control
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cell line: Human umbilical vein smooth muscle cells (HUVSMCs)
agent: control
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Expression data from untreated HUVSMC cell line
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Sample_geo_accession | GSM706153
| Sample_status | Public on Jul 01 2011
| Sample_submission_date | Apr 08 2011
| Sample_last_update_date | Jul 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with 0.3μM of Triptolide for 24 hours.
| Sample_growth_protocol_ch1 | Cells were grown to 85 % confluence in 10 cm tissue culture dish.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_hyb_protocol | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_scan_protocol | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_data_processing | MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
| Sample_platform_id | GPL570
| Sample_contact_name | Ming-Ta,,Hsu
| Sample_contact_email | kentkenpony@hotmail.com
| Sample_contact_institute | National Yang Ming University
| Sample_contact_address | No.155, Sec.2, Linong Street
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 112
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706153/suppl/GSM706153.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706153/suppl/GSM706153.CHP.gz
| Sample_series_id | GSE28502
| Sample_series_id | GSE30350
| Sample_data_row_count | 54675
| |
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GSM706154 | GPL570 |
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SMC-Triptolide
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HUVSMC cell line treated with 0.3μM of Triptolide for 24 hours
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cell line: Human umbilical vein smooth muscle cells (HUVSMCs)
agent: Triptolide
|
Expression data from HUVSMC cell line treated with 0.3 mM of Triptolide for 24 hours
|
Sample_geo_accession | GSM706154
| Sample_status | Public on Jul 01 2011
| Sample_submission_date | Apr 08 2011
| Sample_last_update_date | Jul 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with 0.3μM of Triptolide for 24 hours.
| Sample_growth_protocol_ch1 | Cells were grown to 85 % confluence in 10 cm tissue culture dish.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_hyb_protocol | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_scan_protocol | Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
| Sample_data_processing | MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
| Sample_platform_id | GPL570
| Sample_contact_name | Ming-Ta,,Hsu
| Sample_contact_email | kentkenpony@hotmail.com
| Sample_contact_institute | National Yang Ming University
| Sample_contact_address | No.155, Sec.2, Linong Street
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 112
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706154/suppl/GSM706154.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706154/suppl/GSM706154.CHP.gz
| Sample_series_id | GSE28502
| Sample_series_id | GSE30350
| Sample_data_row_count | 54675
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