Result

Search results for the GEO ID: GSE28503

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM706155

GPL570

Control-SiMT

MCF-7 cell line transfected with non-targeting knockdown control psuper vector

cell line: MCF-7 breast cancer cell line cell line source: 69 year old female genotype/variation: control

Expression data from MCF-7 cell line transfected with non-targeting knockdown control psuper vector

Sample_geo_accession	GSM706155
Sample_status	Public on Jul 01 2011
Sample_submission_date	Apr 08 2011
Sample_last_update_date	Jul 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MCF-7 cell line was subjected to siRNA knockdown of either non-targeting, POLRMT, or PolII for 72 hours
Sample_growth_protocol_ch1	Cells were grown to 85 % confluence in 10 cm tissue culture dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_hyb_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_scan_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_data_processing	MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
Sample_platform_id	GPL570
Sample_contact_name	Ming-Ta,,Hsu
Sample_contact_email	kentkenpony@hotmail.com
Sample_contact_institute	National Yang Ming University
Sample_contact_address	No.155, Sec.2, Linong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706155/suppl/GSM706155.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706155/suppl/GSM706155.CHP.gz
Sample_series_id	GSE28503
Sample_series_id	GSE30350
Sample_data_row_count	54675

GSM706156

GPL570

SiMT-MCF-7

MCF-7 cell line transfected with targeting POLRMT psuper vector

cell line: MCF-7 breast cancer cell line cell line source: 69 year old female genotype/variation: POLRMT knockdown

Expression data from MCF-7 cell line transfected with targeting POLRMT psuper vector

Sample_geo_accession	GSM706156
Sample_status	Public on Jul 01 2011
Sample_submission_date	Apr 08 2011
Sample_last_update_date	Jul 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MCF-7 cell line was subjected to siRNA knockdown of either non-targeting, POLRMT, or PolII for 72 hours
Sample_growth_protocol_ch1	Cells were grown to 85 % confluence in 10 cm tissue culture dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_hyb_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_scan_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_data_processing	MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
Sample_platform_id	GPL570
Sample_contact_name	Ming-Ta,,Hsu
Sample_contact_email	kentkenpony@hotmail.com
Sample_contact_institute	National Yang Ming University
Sample_contact_address	No.155, Sec.2, Linong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706156/suppl/GSM706156.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706156/suppl/GSM706156.CHP.gz
Sample_series_id	GSE28503
Sample_series_id	GSE30350
Sample_data_row_count	54675

GSM706157

GPL570

Control-SiPolII

MCF-7 cell line transfected with non-targeting knockdown control psuper vector

cell line: MCF-7 breast cancer cell line cell line source: 69 year old female genotype/variation: control

Expression data from MCF-7 cell line transfected with non-targeting knockdown control psuper vector

Sample_geo_accession	GSM706157
Sample_status	Public on Jul 01 2011
Sample_submission_date	Apr 08 2011
Sample_last_update_date	Jul 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MCF-7 cell line was subjected to siRNA knockdown of either non-targeting, POLRMT, or PolII for 72 hours
Sample_growth_protocol_ch1	Cells were grown to 85 % confluence in 10 cm tissue culture dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_hyb_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_scan_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_data_processing	MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
Sample_platform_id	GPL570
Sample_contact_name	Ming-Ta,,Hsu
Sample_contact_email	kentkenpony@hotmail.com
Sample_contact_institute	National Yang Ming University
Sample_contact_address	No.155, Sec.2, Linong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706157/suppl/GSM706157.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706157/suppl/GSM706157.CHP.gz
Sample_series_id	GSE28503
Sample_series_id	GSE30350
Sample_data_row_count	54675

GSM706158

GPL570

SiPolII-MCF-7

MCF-7 cell line transfected with targeting PolII psuper vector

cell line: MCF-7 breast cancer cell line cell line source: 69 year old female genotype/variation: PolII knockdown

Expression data from MCF-7 cell line transfected with targeting PolII psuper vector

Sample_geo_accession	GSM706158
Sample_status	Public on Jul 01 2011
Sample_submission_date	Apr 08 2011
Sample_last_update_date	Jul 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	MCF-7 cell line was subjected to siRNA knockdown of either non-targeting, POLRMT, or PolII for 72 hours
Sample_growth_protocol_ch1	Cells were grown to 85 % confluence in 10 cm tissue culture dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each 10 cm dish was washed with 1x PBS for three times. Total RNA was extracted according to the RNeasy® Mini Kit Spin Protocol (QIAGEN). The integrity of the RNA extract was checked by 1.2 % (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_hyb_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_scan_protocol	Affymetrix microarray was performed using Human U133 2.0 (Affymetrix). Details of the methods for RNA quality, sample labeling, hybridization and expression analysis are according to the manual of Affymetrix Microarray Kit and Affymetrix website.
Sample_data_processing	MAS5.0 calculated signal intensities. Using scaling factor. The default settings are "scaling all probe sets to target signal: 500" and "normalization by user defined in value: 1
Sample_platform_id	GPL570
Sample_contact_name	Ming-Ta,,Hsu
Sample_contact_email	kentkenpony@hotmail.com
Sample_contact_institute	National Yang Ming University
Sample_contact_address	No.155, Sec.2, Linong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706158/suppl/GSM706158.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706158/suppl/GSM706158.CHP.gz
Sample_series_id	GSE28503
Sample_series_id	GSE30350
Sample_data_row_count	54675

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