Search results for the GEO ID: GSE28530  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM706664 | GPL1261 |
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Control_siRNA
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mouse embryonic stem cells, control siRNA
|
strain: J1
cell type: mouse embryonic stem (MES) cells
treatment: control siRNA
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Gene expression data from J1 ES cells transfected with control siRNA.
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| Sample_geo_accession | GSM706664
| | Sample_status | Public on Apr 21 2011
| | Sample_submission_date | Apr 11 2011
| | Sample_last_update_date | Apr 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | J1 ES cells were transfected with control siRNA or Tet1 siRNA and harvested four days after transfection.
| | Sample_growth_protocol_ch1 | J1 ES cells were cultured with MEF feeder cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_data_processing | The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706664/suppl/GSM706664.CEL.gz
| | Sample_series_id | GSE28500
| | Sample_series_id | GSE28530
| | Sample_data_row_count | 45101
| | |
|
GSM706665 | GPL1261 |
|
Tet1_siRNA
|
mouse embryonic stem cells, Tet1 siRNA
|
strain: J1
cell type: mouse embryonic stem (MES) cells
treatment: Tet1 siRNA
|
Gene expression data from J1 ES cells transfected with Tet1 siRNA.
|
| Sample_geo_accession | GSM706665
| | Sample_status | Public on Apr 21 2011
| | Sample_submission_date | Apr 11 2011
| | Sample_last_update_date | Apr 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | J1 ES cells were transfected with control siRNA or Tet1 siRNA and harvested four days after transfection.
| | Sample_growth_protocol_ch1 | J1 ES cells were cultured with MEF feeder cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_data_processing | The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706665/suppl/GSM706665.CEL.gz
| | Sample_series_id | GSE28500
| | Sample_series_id | GSE28530
| | Sample_data_row_count | 45101
| | |
|
GSM706666 | GPL1261 |
|
Scr_shRNA
|
mouse embryonic stem cells, Scr shRNA
|
strain: E14
cell type: mouse embryonic stem (MES) cells
treatment: scramble shRNA
|
Gene expression data from E14 ES cells treated with scramble shRNA and selected with puromycin.
|
| Sample_geo_accession | GSM706666
| | Sample_status | Public on Apr 21 2011
| | Sample_submission_date | Apr 11 2011
| | Sample_last_update_date | Apr 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | E14 ES cells were infected with Scr shRNA, Tet1 shRNA2863 or Tet1 shRNA3387 containing lentivirus and harvested after 4-days selection with puromycin.
| | Sample_growth_protocol_ch1 | E14 ES cells were feeder-free cultured.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_data_processing | The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706666/suppl/GSM706666.CEL.gz
| | Sample_series_id | GSE28500
| | Sample_series_id | GSE28530
| | Sample_data_row_count | 45101
| | |
|
GSM706667 | GPL1261 |
|
Tet1_shRNA3387
|
mouse embryonic stem cells, Tet1 shRNA3387
|
strain: E14
cell type: mouse embryonic stem (MES) cells
treatment: Tet1 shRNA3387
|
Gene expression data from E14 ES cells treated with Tet1 shRNA3387 and selected with puromycin.
|
| Sample_geo_accession | GSM706667
| | Sample_status | Public on Apr 21 2011
| | Sample_submission_date | Apr 11 2011
| | Sample_last_update_date | Apr 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | E14 ES cells were infected with Scr shRNA, Tet1 shRNA2863 or Tet1 shRNA3387 containing lentivirus and harvested after 4-days selection with puromycin.
| | Sample_growth_protocol_ch1 | E14 ES cells were feeder-free cultured.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_data_processing | The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706667/suppl/GSM706667.CEL.gz
| | Sample_series_id | GSE28500
| | Sample_series_id | GSE28530
| | Sample_data_row_count | 45101
| | |
|
GSM706668 | GPL1261 |
|
Tet1_shRNA2863
|
mouse embryonic stem cells, Tet1 shRNA2863
|
strain: E14
cell type: mouse embryonic stem (MES) cells
treatment: Tet1 shRNA2863
|
Gene expression data from E14 ES cells treated with Tet1 shRNA2863 and selected with puromycin.
|
| Sample_geo_accession | GSM706668
| | Sample_status | Public on Apr 21 2011
| | Sample_submission_date | Apr 11 2011
| | Sample_last_update_date | Apr 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | E14 ES cells were infected with Scr shRNA, Tet1 shRNA2863 or Tet1 shRNA3387 containing lentivirus and harvested after 4-days selection with puromycin.
| | Sample_growth_protocol_ch1 | E14 ES cells were feeder-free cultured.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_hyb_protocol | Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_scan_protocol | Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
| | Sample_data_processing | The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Feizhen,,Wu
| | Sample_contact_email | wufz@fudan.edu.cn
| | Sample_contact_phone | 86-21-54237821
| | Sample_contact_fax | 86-21-54237876
| | Sample_contact_institute | Fudan Univ, Shanghai, China
| | Sample_contact_address | NO.138 Yi-Xue-Yuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_state | Shanghai
| | Sample_contact_zip/postal_code | 200032
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM706nnn/GSM706668/suppl/GSM706668.CEL.gz
| | Sample_series_id | GSE28500
| | Sample_series_id | GSE28530
| | Sample_data_row_count | 45101
| | |
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