Search results for the GEO ID: GSE28548  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM707024 | GPL570 |
|
Control cells unstimulated, biological rep1
|
HEK293 cells unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells unstimulated
|
| Sample_geo_accession | GSM707024
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707024/suppl/GSM707024.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707025 | GPL570 |
|
Control cells unstimulated, biological rep2
|
HEK293 cells unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells unstimulated
|
| Sample_geo_accession | GSM707025
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707025/suppl/GSM707025.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707026 | GPL570 |
|
Control cells unstimulated, biological rep3
|
HEK293 cells unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells unstimulated
|
| Sample_geo_accession | GSM707026
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707026/suppl/GSM707026.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707027 | GPL570 |
|
Control cells stimulated with TNFa, biological rep1
|
HEK293 cells stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells stimulated with TNFa
|
| Sample_geo_accession | GSM707027
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707027/suppl/GSM707027.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707028 | GPL570 |
|
Control cells stimulated with TNFa, biological rep2
|
HEK293 cells stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells stimulated with TNFa
|
| Sample_geo_accession | GSM707028
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707028/suppl/GSM707028.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707029 | GPL570 |
|
Control cells stimulated with TNFa, biological rep3
|
HEK293 cells stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells stimulated with TNFa
|
| Sample_geo_accession | GSM707029
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707029/suppl/GSM707029.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707030 | GPL570 |
|
Cells transfected with sirtuin 6, biological rep1
|
HEK293 cells transfected with SIRT6 construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and unstimulated
|
| Sample_geo_accession | GSM707030
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707030/suppl/GSM707030.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707031 | GPL570 |
|
Cells transfected with sirtuin 6, biological rep2
|
HEK293 cells transfected with SIRT6 construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and unstimulated
|
| Sample_geo_accession | GSM707031
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707031/suppl/GSM707031.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707032 | GPL570 |
|
Cells transfected with sirtuin 6, biological rep3
|
HEK293 cells transfected with SIRT6 construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and unstimulated
|
| Sample_geo_accession | GSM707032
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707032/suppl/GSM707032.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707033 | GPL570 |
|
Cells transfected with sirtuin 6 and stimulated with TNFa, biological rep1
|
HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707033
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707033/suppl/GSM707033.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707034 | GPL570 |
|
Cells transfected with sirtuin 6 and stimulated with TNFa, biological rep2
|
HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707034
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707034/suppl/GSM707034.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707035 | GPL570 |
|
Cells transfected with sirtuin 6 and stimulated with TNFa, biological rep3
|
HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with SIRT6 construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707035
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707035/suppl/GSM707035.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707036 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6, biological rep1
|
HEK293 cells transfected with H133W construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and unstimulated
|
| Sample_geo_accession | GSM707036
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707036/suppl/GSM707036.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707037 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6, biological rep2
|
HEK293 cells transfected with H133W construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and unstimulated
|
| Sample_geo_accession | GSM707037
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707037/suppl/GSM707037.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707038 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6, biological rep3
|
HEK293 cells transfected with H133W construct and unstimulated
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and unstimulated
|
| Sample_geo_accession | GSM707038
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707038/suppl/GSM707038.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707039 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6 and stimulated with TNFa, biological rep1
|
HEK293 cells transfected with H133W construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707039
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707039/suppl/GSM707039.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707040 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6 and stimulated with TNFa, biological rep2
|
HEK293 cells transfected with H133W construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707040
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707040/suppl/GSM707040.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707041 | GPL570 |
|
Cells transfected with H133W mutant sirtuin 6 and stimulated with TNFa, biological rep3
|
HEK293 cells transfected with H133W construct and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with H133W construct and stimulated with TNFa
|
| Sample_geo_accession | GSM707041
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707041/suppl/GSM707041.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707042 | GPL570 |
|
Cells transfected with pCDNA3, biological rep1
|
HEK293 cells tranfected with with an pCDNA3
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3
|
| Sample_geo_accession | GSM707042
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707042/suppl/GSM707042.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707043 | GPL570 |
|
Cells transfected with pCDNA3, biological rep2
|
HEK293 cells tranfected with with an pCDNA3
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3
|
| Sample_geo_accession | GSM707043
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707043/suppl/GSM707043.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707044 | GPL570 |
|
Cells transfected with pCDNA3, biological rep3
|
HEK293 cells tranfected with with an pCDNA3
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3
|
| Sample_geo_accession | GSM707044
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707044/suppl/GSM707044.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707045 | GPL570 |
|
Cells transfected with pCDNA3 and stimulated with TNFa, biological rep1
|
HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
| Sample_geo_accession | GSM707045
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707045/suppl/GSM707045.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707046 | GPL570 |
|
Cells transfected with pCDNA3 and stimulated with TNFa, biological rep2
|
HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
| Sample_geo_accession | GSM707046
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707046/suppl/GSM707046.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
GSM707047 | GPL570 |
|
Cells transfected with pCDNA3 and stimulated with TNFa, biological rep3
|
HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
cell line: HEK293
|
Gene expression data from HEK293 cells tranfected with with an pCDNA3 and stimulated with TNFa
|
| Sample_geo_accession | GSM707047
| | Sample_status | Public on Jul 31 2011
| | Sample_submission_date | Apr 12 2011
| | Sample_last_update_date | Jul 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transfected with pCDNA3-Flag-SIRT6 WT, pCDNA3-Flag-SIRT6 H133W mutant, pCDNA3 alone or no DNA. Cells were grown in media containing transfection complexes for 24h prior to stimulation. Growth media was removed and replaced with fresh media containing TNFα (10ng/ml, R&D systems) or media containing equivalent volume of PBS/0.1% BSA as a control.
| | Sample_growth_protocol_ch1 | HEK293 cells were plated at 0.4 x 106 cells per well in 6-well plates (Costar)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated, processed, and hybridized to the oligonucleotide microarray HG 133A Plus 2.0 Gene Chip arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 3.75 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Fluorescence raw data of the hybridization were computed, RMA normalized, log2 transformed and statistically analyzed using ArrayStudio v4.0 (Omicsoft).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jessica,,Vamathevan
| | Sample_contact_email | jessica.j.vamathevan@gsk.com
| | Sample_contact_department | R&D
| | Sample_contact_institute | GlaxoSmithKline
| | Sample_contact_address | Gunnels Wood Road
| | Sample_contact_city | Stevenage
| | Sample_contact_zip/postal_code | SG1 2NY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM707nnn/GSM707047/suppl/GSM707047.CEL.gz
| | Sample_series_id | GSE28548
| | Sample_data_row_count | 54675
| | |
|
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