Result

Search results for the GEO ID: GSE28618

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GSM ID

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Title

Source name

Description

Characteristics

GSM708667

GPL570

Control siRNA

U87 glioma cells transfected with control siRNA

cell line: U87 knockdown: control

Sample_geo_accession	GSM708667
Sample_status	Public on Apr 16 2011
Sample_submission_date	Apr 14 2011
Sample_last_update_date	Apr 16 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Control or HOXD9 siRNA-2 were transfected at a concentration of 50 nM using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions.
Sample_growth_protocol_ch1	U87 cells were seeded in tissue culture dishes 48 h prior to RNAi transfection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	U87 cells were used for total RNA extraction using Trizol(Invitrogen) and RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChipOperationSoftware (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Masanao,,Tabuse
Sample_contact_email	booboo-m@khaki.plala.or.jp
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinanomachi
Sample_contact_city	Shinjuku-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM708nnn/GSM708667/suppl/GSM708667_U87_haito.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM708nnn/GSM708667/suppl/GSM708667_U87_haito1.CHP.gz
Sample_series_id	GSE28618
Sample_data_row_count	54675

GSM709339

GPL570

HOXD9 siRNA

U87 glioma cells transfected with HOXD9 siRNA

cell line: U87 knockdown: HOXD9

Sample_geo_accession	GSM709339
Sample_status	Public on Apr 16 2011
Sample_submission_date	Apr 14 2011
Sample_last_update_date	Apr 16 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Control or HOXD9 siRNA-2 were transfected at a concentration of 50 nM using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions.
Sample_growth_protocol_ch1	U87 cells were seeded in tissue culture dishes 48 h prior to RNAi transfection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	U87 cells were used for total RNA extraction using Trizol(Invitrogen) and RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneArray Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChipOperationSoftware (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Masanao,,Tabuse
Sample_contact_email	booboo-m@khaki.plala.or.jp
Sample_contact_institute	Keio University School of Medicine
Sample_contact_address	35 Shinanomachi
Sample_contact_city	Shinjuku-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	160-8582
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709339/suppl/GSM709339_U87_HOXD9.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709339/suppl/GSM709339_U87_HOXD9.CHP.gz
Sample_series_id	GSE28618
Sample_data_row_count	54675

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