Search results for the GEO ID: GSE28646 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM709778 | GPL570 |
|
A2780, biological replicate 1
|
Ovarian Cisplatin sensitive cell line A2780
|
cell line: A2780
|
|
Sample_geo_accession | GSM709778
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709778/suppl/GSM709778.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709779 | GPL570 |
|
A2780, biological replicate 2
|
Ovarian Cisplatin sensitive cell line A2780
|
cell line: A2780
|
|
Sample_geo_accession | GSM709779
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709779/suppl/GSM709779.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709780 | GPL570 |
|
A2780, biological replicate 3
|
Ovarian Cisplatin sensitive cell line A2780
|
cell line: A2780
|
|
Sample_geo_accession | GSM709780
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709780/suppl/GSM709780.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709781 | GPL570 |
|
CP70, biological replicate 1
|
Ovarian Cisplatin resistant cell line CP70
|
cell line: CP70
|
|
Sample_geo_accession | GSM709781
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709781/suppl/GSM709781.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709782 | GPL570 |
|
CP70, biological replicate 2
|
Ovarian Cisplatin resistant cell line CP70
|
cell line: CP70
|
|
Sample_geo_accession | GSM709782
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709782/suppl/GSM709782.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709783 | GPL570 |
|
CP70, biological replicate 3
|
Ovarian Cisplatin resistant cell line CP70
|
cell line: CP70
|
|
Sample_geo_accession | GSM709783
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709783/suppl/GSM709783.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709784 | GPL570 |
|
CP70 treated with Decitabine, biological replicate 1
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine
|
cell line: CP70
|
|
Sample_geo_accession | GSM709784
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709784/suppl/GSM709784.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709785 | GPL570 |
|
CP70 treated with Decitabine, biological replicate 2
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine
|
cell line: CP70
|
|
Sample_geo_accession | GSM709785
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709785/suppl/GSM709785.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709786 | GPL570 |
|
CP70 treated with Decitabine, biological replicate 3
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine
|
cell line: CP70
|
|
Sample_geo_accession | GSM709786
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709786/suppl/GSM709786.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709787 | GPL570 |
|
CP70 treated with Decitabine and PXD101, biological replicate 1
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine and PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709787
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709787/suppl/GSM709787.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709788 | GPL570 |
|
CP70 treated with Decitabine and PXD101, biological replicate 2
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine and PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709788
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709788/suppl/GSM709788.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709789 | GPL570 |
|
CP70 treated with Decitabine and PXD101, biological replicate 3
|
Ovarian Cisplatin resisant cell line CP70 treated with Decitabine and PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709789
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709789/suppl/GSM709789.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709790 | GPL570 |
|
CP70 treated with PXD101, biological replicate 1
|
Ovarian Cisplatin resisant cell line CP70 treated with PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709790
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709790/suppl/GSM709790.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709791 | GPL570 |
|
CP70 treated with PXD101, biological replicate 2
|
Ovarian Cisplatin resisant cell line CP70 treated with PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709791
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709791/suppl/GSM709791.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
GSM709792 | GPL570 |
|
CP70 treated with PXD101, biological replicate 3
|
Ovarian Cisplatin resisant cell line CP70 treated with PXD101
|
cell line: CP70
|
|
Sample_geo_accession | GSM709792
| Sample_status | Public on Dec 20 2011
| Sample_submission_date | Apr 15 2011
| Sample_last_update_date | Dec 20 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | A2780/cp70 +Decitabine: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with Decitabine being replaced after 24h. Cells were harvested 96 h following drug removal. A2780/cp70 +PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM PXD101 for 48 h. Cells were harvested 96 h following drug removal. A2780/cp80 +Decitabine+PXD101: A2780/cp70 cells were treated at 50% confluence with 0.1 µM Decitabine for 48 h with 0.1 µM PXD101 being added for an additional 24 hours following the initial 24 hour DAC treatment. Cells were harvested 96 h following drug removal.
| Sample_growth_protocol_ch1 | Cell line was grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted and purified from cell lines using the Qiagen RNAeasy kit according to standard instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide).
| Sample_hyb_protocol | The NuGEN hybridization v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual.
| Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| Sample_data_processing | The data were analyzed in R (version 2.10.1) using Affy package and quantile normalization as the normalisation method.
| Sample_platform_id | GPL570
| Sample_contact_name | Wei,,Dai
| Sample_contact_email | w.dai@imperial.ac.uk
| Sample_contact_laboratory | Epigenetics
| Sample_contact_department | Sugery and Cancer
| Sample_contact_institute | Imperial College London
| Sample_contact_address | Du Cane Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | W12 0NN
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709792/suppl/GSM709792.CEL.gz
| Sample_series_id | GSE28646
| Sample_series_id | GSE28648
| Sample_data_row_count | 54675
| |
|
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Select GSMs and click on "Add groups" |
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