Search results for the GEO ID: GSE28651  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM709832 | GPL1261 |
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sorted basal cells, biological rep 1
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TROP-2+ITGA6+ sorted cells from dispase digested surface epithelium
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cell type: proximal airway basal cell
tracheal section: lower two-thirds
strain: C57BL/6
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C57BL/6
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| Sample_geo_accession | GSM709832
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells from proximal airway surface epithelium were stained for TROP-2 and ITGA6 conjugated to PE or APC (eBioscience and Biolegend) and cells from SMG were stained for TROP-2 by incubation with the antibody for 15 minutes at room temperature followed by washing and incubation in donkey anti-goat Alexa Fluor 488 or PE. Sorting was performed on a FACS Aria and the data analyzed with FACS Diva software (BD Biosciences).
| | Sample_growth_protocol_ch1 | Single cell suspensions were generated from enzymatic digestion and manual dissection of proximal airways.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Afffymetrix protocol
| | Sample_hyb_protocol | Standard Afffymetrix protocol
| | Sample_scan_protocol | Standard Afffymetrix protocol
| | Sample_data_processing | Affymetrix AGCC
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Brigitte,,Gomperts
| | Sample_contact_email | BGomperts@mednet.ucla.edu
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10833 Le Conte Ave., A2-410 MDCC
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709832/suppl/GSM709832_B1_N4_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709832/suppl/GSM709832_B1_N4_.mas5.CHP.gz
| | Sample_series_id | GSE28651
| | Sample_data_row_count | 45101
| | |
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GSM709833 | GPL1261 |
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sorted basal cells, biological rep 2
|
TROP-2+ITGA6+ sorted cells from dispase digested surface epithelium
|
cell type: proximal airway basal cell
tracheal section: lower two-thirds
strain: C57BL/6
|
C57BL/6
|
| Sample_geo_accession | GSM709833
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells from proximal airway surface epithelium were stained for TROP-2 and ITGA6 conjugated to PE or APC (eBioscience and Biolegend) and cells from SMG were stained for TROP-2 by incubation with the antibody for 15 minutes at room temperature followed by washing and incubation in donkey anti-goat Alexa Fluor 488 or PE. Sorting was performed on a FACS Aria and the data analyzed with FACS Diva software (BD Biosciences).
| | Sample_growth_protocol_ch1 | Single cell suspensions were generated from enzymatic digestion and manual dissection of proximal airways.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Afffymetrix protocol
| | Sample_hyb_protocol | Standard Afffymetrix protocol
| | Sample_scan_protocol | Standard Afffymetrix protocol
| | Sample_data_processing | Affymetrix AGCC
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Brigitte,,Gomperts
| | Sample_contact_email | BGomperts@mednet.ucla.edu
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10833 Le Conte Ave., A2-410 MDCC
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709833/suppl/GSM709833_B2_N6_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709833/suppl/GSM709833_B2_N6_.mas5.CHP.gz
| | Sample_series_id | GSE28651
| | Sample_data_row_count | 45101
| | |
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GSM709834 | GPL1261 |
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sorted duct cells, biological rep 1
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TROP-2+ sorted cells from tracheal tissue after surface epithelium removed with pronase
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cell type: proximal airway duct cell
tracheal section: upper third
strain: C57BL/6
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C57BL/6
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| Sample_geo_accession | GSM709834
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells from proximal airway surface epithelium were stained for TROP-2 and ITGA6 conjugated to PE or APC (eBioscience and Biolegend) and cells from SMG were stained for TROP-2 by incubation with the antibody for 15 minutes at room temperature followed by washing and incubation in donkey anti-goat Alexa Fluor 488 or PE. Sorting was performed on a FACS Aria and the data analyzed with FACS Diva software (BD Biosciences).
| | Sample_growth_protocol_ch1 | Single cell suspensions were generated from enzymatic digestion and manual dissection of proximal airways.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Afffymetrix protocol
| | Sample_hyb_protocol | Standard Afffymetrix protocol
| | Sample_scan_protocol | Standard Afffymetrix protocol
| | Sample_data_processing | Affymetrix AGCC
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Brigitte,,Gomperts
| | Sample_contact_email | BGomperts@mednet.ucla.edu
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10833 Le Conte Ave., A2-410 MDCC
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709834/suppl/GSM709834_D1_N1_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709834/suppl/GSM709834_D1_N1_.mas5.CHP.gz
| | Sample_series_id | GSE28651
| | Sample_data_row_count | 45101
| | |
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GSM709835 | GPL1261 |
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sorted duct cells, biological rep 2
|
TROP-2+ sorted cells from tracheal tissue after surface epithelium removed with pronase
|
cell type: proximal airway duct cell
tracheal section: upper third
strain: C57BL/6
|
C57BL/6
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| Sample_geo_accession | GSM709835
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells from proximal airway surface epithelium were stained for TROP-2 and ITGA6 conjugated to PE or APC (eBioscience and Biolegend) and cells from SMG were stained for TROP-2 by incubation with the antibody for 15 minutes at room temperature followed by washing and incubation in donkey anti-goat Alexa Fluor 488 or PE. Sorting was performed on a FACS Aria and the data analyzed with FACS Diva software (BD Biosciences).
| | Sample_growth_protocol_ch1 | Single cell suspensions were generated from enzymatic digestion and manual dissection of proximal airways.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Afffymetrix protocol
| | Sample_hyb_protocol | Standard Afffymetrix protocol
| | Sample_scan_protocol | Standard Afffymetrix protocol
| | Sample_data_processing | Affymetrix AGCC
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Brigitte,,Gomperts
| | Sample_contact_email | BGomperts@mednet.ucla.edu
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10833 Le Conte Ave., A2-410 MDCC
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709835/suppl/GSM709835_D3_N2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709835/suppl/GSM709835_D3_N2_.mas5.CHP.gz
| | Sample_series_id | GSE28651
| | Sample_data_row_count | 45101
| | |
|
GSM709836 | GPL1261 |
|
sorted duct cells, biological rep 3
|
TROP-2+ sorted cells from tracheal tissue after surface epithelium removed with pronase
|
cell type: proximal airway duct cell
tracheal section: upper third
strain: C57BL/6
|
C57BL/6
|
| Sample_geo_accession | GSM709836
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells from proximal airway surface epithelium were stained for TROP-2 and ITGA6 conjugated to PE or APC (eBioscience and Biolegend) and cells from SMG were stained for TROP-2 by incubation with the antibody for 15 minutes at room temperature followed by washing and incubation in donkey anti-goat Alexa Fluor 488 or PE. Sorting was performed on a FACS Aria and the data analyzed with FACS Diva software (BD Biosciences).
| | Sample_growth_protocol_ch1 | Single cell suspensions were generated from enzymatic digestion and manual dissection of proximal airways.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Afffymetrix protocol
| | Sample_hyb_protocol | Standard Afffymetrix protocol
| | Sample_scan_protocol | Standard Afffymetrix protocol
| | Sample_data_processing | Affymetrix AGCC
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Brigitte,,Gomperts
| | Sample_contact_email | BGomperts@mednet.ucla.edu
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10833 Le Conte Ave., A2-410 MDCC
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709836/suppl/GSM709836_D4_N3_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM709nnn/GSM709836/suppl/GSM709836_D4_N3_.mas5.CHP.gz
| | Sample_series_id | GSE28651
| | Sample_data_row_count | 45101
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