Search results for the GEO ID: GSE28662  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM710149 | GPL3921 |
|
DMSO 2 hours, replicate 1
|
Control cells at 2h
|
cell line: MCF7
compound: Control
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710149
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710149/suppl/GSM710149.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710150 | GPL3921 |
|
DMSO 2 hours, replicate 2
|
Control cells at 2h
|
cell line: MCF7
compound: Control
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710150
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710150/suppl/GSM710150.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710151 | GPL3921 |
|
DMSO 4 hours, replicate 1
|
Control cells at 4h
|
cell line: MCF7
compound: Control
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710151
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710151/suppl/GSM710151.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710152 | GPL3921 |
|
DMSO 4 hours, replicate 2
|
Control cells at 4h
|
cell line: MCF7
compound: Control
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710152
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710152/suppl/GSM710152.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710153 | GPL3921 |
|
DMSO 6 hours, replicate 1
|
Control cells at 6h
|
cell line: MCF7
compound: Control
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710153
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710153/suppl/GSM710153.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710154 | GPL3921 |
|
DMSO 6 hours, replicate 2
|
Control cells at 6h
|
cell line: MCF7
compound: Control
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710154
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710154/suppl/GSM710154.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710155 | GPL3921 |
|
Actinomycin D, 2 hours, replicate 1
|
Actinomycin D treated for 2 hours
|
cell line: MCF7
compound: Actinomycin D
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710155
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710155/suppl/GSM710155.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710156 | GPL3921 |
|
Actinomycin D, 2 hours, replicate 2
|
Actinomycin D treated for 2 hours
|
cell line: MCF7
compound: Actinomycin D
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710156
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710156/suppl/GSM710156.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710157 | GPL3921 |
|
Actinomycin D, 4 hours, replicate 1
|
Actinomycin D treated for 4 hours
|
cell line: MCF7
compound: Actinomycin D
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710157
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710157/suppl/GSM710157.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710158 | GPL3921 |
|
Actinomycin D, 4 hours, replicate 2
|
Actinomycin D treated for 4 hours
|
cell line: MCF7
compound: Actinomycin D
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710158
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710158/suppl/GSM710158.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710159 | GPL3921 |
|
Actinomycin D, 6 hours, replicate 1
|
Actinomycin D treated for 6 hours
|
cell line: MCF7
compound: Actinomycin D
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710159
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710159/suppl/GSM710159.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710160 | GPL3921 |
|
Actinomycin D, 6 hours, replicate 2
|
Actinomycin D treated for 6 hours
|
cell line: MCF7
compound: Actinomycin D
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710160
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710160/suppl/GSM710160.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710161 | GPL3921 |
|
Triptolide, 2 hours, replicate 1
|
triptolide treated for 2 hours
|
cell line: MCF7
compound: Triptolide
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710161
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710161/suppl/GSM710161.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710162 | GPL3921 |
|
Triptolide, 2 hours, replicate 2
|
triptolide treated for 2 hours
|
cell line: MCF7
compound: Triptolide
time: 2 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710162
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710162/suppl/GSM710162.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710163 | GPL3921 |
|
Triptolide, 4 hours, replicate 1
|
triptolide treated for 4 hours
|
cell line: MCF7
compound: Triptolide
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710163
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710163/suppl/GSM710163.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710164 | GPL3921 |
|
Triptolide, 4 hours, replicate 2
|
triptolide treated for 4 hours
|
cell line: MCF7
compound: Triptolide
time: 4 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710164
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710164/suppl/GSM710164.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710165 | GPL3921 |
|
Triptolide, 6 hours, replicate 1
|
triptolide treated for 6 hours
|
cell line: MCF7
compound: Triptolide
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710165
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710165/suppl/GSM710165.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
|
GSM710166 | GPL3921 |
|
Triptolide, 6 hours, replicate 2
|
triptolide treated for 6 hours
|
cell line: MCF7
compound: Triptolide
time: 6 hours
|
Gene expression after treatment
|
| Sample_geo_accession | GSM710166
| | Sample_status | Public on Oct 18 2011
| | Sample_submission_date | Apr 15 2011
| | Sample_last_update_date | Oct 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with actinomycin D at 2.5 uM or triptolide at 500 nM.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured with RPMI-1640 media supplemented with 10% FBS, 2 mM glutamine, and 1X penn/strp.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First strand cDNA synthesis was generated using a T7-linked oligo-dT primer, followed by second strand synthesis. An in vitro transcription reaction was performed to generate cRNA containing biotinylated UTP and CTP, which was subsequently chemically fragmented at 95°C for 35 minutes.
| | Sample_hyb_protocol | Ten micrograms of the fragmented, biotinylated cRNA was hybridized in MES buffer (2-[N-Morpholino]ethansulfonic acid) containing 0.5 mg/ml acetylated bovine serum albumin (Sigma) to Affymetrix U133A arrays at 45°C for 16 hours.
| | Sample_scan_protocol | Scans were performed on Affymetrix scanners.
| | Sample_data_processing | Raw expression values were normalized using Robust Multiarray Averaging (RMA).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Guo,,Wei
| | Sample_contact_email | guowei@broadinstitute.org
| | Sample_contact_institute | Broad Institute or MIT and Harvard
| | Sample_contact_address | 7 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM710nnn/GSM710166/suppl/GSM710166.CEL.gz
| | Sample_series_id | GSE28662
| | Sample_data_row_count | 22277
| | |
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