Search results for the GEO ID: GSE28715  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM711410 | GPL570 |
|
HEK293Tcells_wt_rep1
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Dharmacon non-targeting #2
|
Gene expression from HEK293T cells after transfection with non-targeting siRNA, replicate 1.
|
| Sample_geo_accession | GSM711410
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711410/suppl/GSM711410_cont-1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711411 | GPL570 |
|
HEK293Tcells_wt_rep2
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Dharmacon non-targeting #3
|
Gene expression from HEK293T cells after transfection with non-targeting siRNA, replicate 2.
|
| Sample_geo_accession | GSM711411
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711411/suppl/GSM711411_cont-2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711412 | GPL570 |
|
HEK293Tcells_wt_rep3
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Dharmacon non-targeting #4
|
Gene expression from HEK293T cells after transfection with non-targeting siRNA, replicate 3.
|
| Sample_geo_accession | GSM711412
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711412/suppl/GSM711412_cont-3_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711413 | GPL570 |
|
HEK293Tcells_si74_rep1
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18074
|
Gene expression from HEK293T cells after transfection with siRNA s18074 targeting Med26, replicate 1.
|
| Sample_geo_accession | GSM711413
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711413/suppl/GSM711413_si-74-1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711414 | GPL570 |
|
HEK293Tcells_si74_rep2
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18074
|
Gene expression from HEK293T cells after transfection with siRNA s18074 targeting Med26, replicate 2.
|
| Sample_geo_accession | GSM711414
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711414/suppl/GSM711414_si-74-2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711415 | GPL570 |
|
HEK293Tcells_si74_rep3
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18074
|
Gene expression from HEK293T cells after transfection with siRNA s18074 targeting Med26, replicate 3.
|
| Sample_geo_accession | GSM711415
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711415/suppl/GSM711415_si-74-3_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711416 | GPL570 |
|
HEK293Tcells_si75_rep1
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18075
|
Gene expression from HEK293T cells after transfection with siRNA s18075 targeting Med26, replicate 1.
|
| Sample_geo_accession | GSM711416
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711416/suppl/GSM711416_si-75-1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711417 | GPL570 |
|
HEK293Tcells_si75_rep2
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18075
|
Gene expression from HEK293T cells after transfection with siRNA s18075 targeting Med26, replicate 2.
|
| Sample_geo_accession | GSM711417
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711417/suppl/GSM711417_si-75-2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711418 | GPL570 |
|
HEK293Tcells_si75_rep3
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18075
|
Gene expression from HEK293T cells after transfection with siRNA s18075 targeting Med26, replicate 3.
|
| Sample_geo_accession | GSM711418
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711418/suppl/GSM711418_si-75-3_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711419 | GPL570 |
|
HEK293Tcells_si76_rep1
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18076
|
Gene expression from HEK293T cells after transfection with siRNA s18076 targeting Med26, replicate 1.
|
| Sample_geo_accession | GSM711419
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711419/suppl/GSM711419_si-76-1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711420 | GPL570 |
|
HEK293Tcells_si76_rep2
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HEK293T cells
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cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18076
|
Gene expression from HEK293T cells after transfection with siRNA s18076 targeting Med26, replicate 2.
|
| Sample_geo_accession | GSM711420
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711420/suppl/GSM711420_si-76-2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
|
GSM711421 | GPL570 |
|
HEK293Tcells_si76_rep3
|
HEK293T cells
|
cell line: HEK293T
cell type: human embryonic kidney
sirna: Med26 targeting s18076
|
Gene expression from HEK293T cells after transfection with siRNA s18076 targeting Med26, replicate 3.
|
| Sample_geo_accession | GSM711421
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Apr 19 2011
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293T cells in 6 well tissue culture plates (~1x105 cells/well) or 10 cm dishes (~2 x106 cells/dish) were transfected with 25 nM siRNAs targeting human Med26 (Ambion/Applied Biosystems, #4, s18074; #5, s18075; #6, s18076) or 25 nM siCONTROL NON-TARGETING siRNA #2 (Dharmacon D-001210-01) using X-tremeGENE siRNA Transfection Reagent (Roche) and grown for 48 hours.
| | Sample_growth_protocol_ch1 | HEK293T cells were grown in 6 well tissue culture plates or 10 cm dishes for 48 hours after transfection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the standard Qiagen RNeasy cleanup protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Ambion MessageAmp III protocol from 300 ng total RNA (MessageAmp III Amplification kit Manual, 2007, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 and processed with AGCC v3.0 software.
| | Sample_data_processing | Array intensities were processed using the RMA algorithm in R/bioconductor. Bioconductor 2.6 and affy package affy_1.24.2
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,W,Seidel
| | Sample_contact_email | seidel@phageT4.org
| | Sample_contact_phone | 816 926 9054
| | Sample_contact_laboratory | Seidel
| | Sample_contact_department | Genomics
| | Sample_contact_institute | Stowers Institute
| | Sample_contact_address | 1000 E 50th St
| | Sample_contact_city | Kansas City
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 64110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM711nnn/GSM711421/suppl/GSM711421_si-76-3_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE28715
| | Sample_data_row_count | 54675
| | |
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