Search results for the GEO ID: GSE28783  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM712670 | GPL1261 |
|
macrophage, anti-miR-33 treated, biological rep 1
|
plaque macrophage anti-miR-33 treated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with anti-miR-33
|
| Sample_geo_accession | GSM712670
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712670/suppl/GSM712670.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712671 | GPL1261 |
|
macrophage, anti-miR-33 treated, biological rep 2
|
plaque macrophage anti-miR-33 treated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with anti-miR-33
|
| Sample_geo_accession | GSM712671
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712671/suppl/GSM712671.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712672 | GPL1261 |
|
macrophage, anti-miR-33 treated, biological rep 3
|
plaque macrophage anti-miR-33 treated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with anti-miR-33
|
| Sample_geo_accession | GSM712672
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712672/suppl/GSM712672.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712673 | GPL1261 |
|
macrophage, anti-miR-33 treated, biological rep 4
|
plaque macrophage anti-miR-33 treated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with anti-miR-33
|
| Sample_geo_accession | GSM712673
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712673/suppl/GSM712673.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712674 | GPL1261 |
|
macrophage, control anti-miR, biological rep 1
|
plaque macrophage control anti-miR
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with control anti-miR
|
| Sample_geo_accession | GSM712674
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712674/suppl/GSM712674.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712675 | GPL1261 |
|
macrophage, control anti-miR, biological rep 2
|
plaque macrophage control anti-miR
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with control anti-miR
|
| Sample_geo_accession | GSM712675
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712675/suppl/GSM712675.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712676 | GPL1261 |
|
macrophage, control anti-miR, biological rep 3
|
plaque macrophage control anti-miR
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with control anti-miR
|
| Sample_geo_accession | GSM712676
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712676/suppl/GSM712676.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712677 | GPL1261 |
|
macrophage, control anti-miR, biological rep 4
|
plaque macrophage control anti-miR
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with control anti-miR
|
| Sample_geo_accession | GSM712677
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712677/suppl/GSM712677.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712678 | GPL1261 |
|
macrophage, untreated, biological rep 1
|
plaque macrophage untreated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with saline
|
| Sample_geo_accession | GSM712678
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712678/suppl/GSM712678.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712679 | GPL1261 |
|
macrophage, untreated, biological rep 2
|
plaque macrophage untreated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with saline
|
| Sample_geo_accession | GSM712679
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712679/suppl/GSM712679.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712680 | GPL1261 |
|
macrophage, untreated, biological rep 3
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plaque macrophage untreated
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strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
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Gene expression data from plaque macrophages treated with saline
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| Sample_geo_accession | GSM712680
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712680/suppl/GSM712680.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
| | |
|
GSM712681 | GPL1261 |
|
macrophage, untreated, biological rep 4
|
plaque macrophage untreated
|
strain: C57Bl/6
gender: male
tissue: macrophage
genotype: LDLR knockout
|
Gene expression data from plaque macrophages treated with saline
|
| Sample_geo_accession | GSM712681
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | Apr 21 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice received 2 injections of 10 mg/kg anti-miR or control (or an equivalent volume of PBS) the first week, spaced two days apart, and weekly injections of 10 mg/kg anti-miR (or PBS) thereafter for 4 weeks.
| | Sample_growth_protocol_ch1 | Ldlr-/- mice were weaned at 4 weeks of age and placed on a high fat diet (21% (wt/wt) fat, 0.3% cholesterol; Research Diets) for 14 weeks, at which point mice were switched to chow diet for 4 weeks and anti-miR treatment began.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting with 1 ug total RNA, produce amplified biotin-labeled cRNA targets using the Enzo Target Labeling method.
| | Sample_hyb_protocol | Hybridize fragmented, biotin-labeled cRNA to the Affymetrix GeneChip® Array overnight at 45oC for 16 to 18 hours (GeneChip Hybridization Oven 640) according to the manufacturer’s recommendations.
| | Sample_scan_protocol | Stain, wash (GeneChip Fluidics Station 450, EukGE-WS2v5_450 script) and scan (GCS 3000 7G with the GeneChip® AutoLoader) performed according to the manufacturer’s recommendations.
| | Sample_data_processing | The data were analyzed with Array Studio version 4.1 using Affymetrix default analysis settings and RMA as normalization method. The normalized signals were log-2 transformed.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christine,,Esau
| | Sample_contact_email | cesau@regulusrx.com
| | Sample_contact_institute | Regulus Therapeutics
| | Sample_contact_address | 3545 John Hopkins Court
| | Sample_contact_city | San Diego
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92121
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM712nnn/GSM712681/suppl/GSM712681.CEL.gz
| | Sample_series_id | GSE28783
| | Sample_data_row_count | 45101
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