Search results for the GEO ID: GSE28810  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM713416 | GPL570 |
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U251 control
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U251-Control
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cell line: U251
cell type: glioma
mir-31 transfection: control
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U251 cells transfected with control
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| Sample_geo_accession | GSM713416
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Apr 22 2011
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM cell culture media with 10%FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with trizol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit (Lot no. 901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The chips were scanned with the affymetrix genechip 7G plus, and AGCC is used for the image processing.
| | Sample_data_processing | Genespring 10.0, is used for automatically normalizing the chip data and analyzing the fold change.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Biao Yang,,Lin
| | Sample_contact_laboratory | Systems Biology Division
| | Sample_contact_department | ZCNI
| | Sample_contact_institute | ZheJiang University
| | Sample_contact_address | 268 Kaixuan Road
| | Sample_contact_city | HangZhou
| | Sample_contact_zip/postal_code | 310029
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM713nnn/GSM713416/suppl/GSM713416_U251-Control.CEL.gz
| | Sample_series_id | GSE28810
| | Sample_data_row_count | 54675
| | |
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GSM713417 | GPL570 |
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U251 miR-31 overexpression
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U251-miR-31
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cell line: U251
cell type: glioma
mir-31 transfection: has-miR-31
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U251 cells transfected with has-miR-31
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| Sample_geo_accession | GSM713417
| | Sample_status | Public on Sep 01 2012
| | Sample_submission_date | Apr 22 2011
| | Sample_last_update_date | Sep 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM cell culture media with 10%FBS
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with trizol according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, GeneChip 3 IVT Express Kit (Lot no. 901228) is used in the experiment.
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The chips were scanned with the affymetrix genechip 7G plus, and AGCC is used for the image processing.
| | Sample_data_processing | Genespring 10.0, is used for automatically normalizing the chip data and analyzing the fold change.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Biao Yang,,Lin
| | Sample_contact_laboratory | Systems Biology Division
| | Sample_contact_department | ZCNI
| | Sample_contact_institute | ZheJiang University
| | Sample_contact_address | 268 Kaixuan Road
| | Sample_contact_city | HangZhou
| | Sample_contact_zip/postal_code | 310029
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM713nnn/GSM713417/suppl/GSM713417_U251-miR-31.CEL.gz
| | Sample_series_id | GSE28810
| | Sample_data_row_count | 54675
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