Search results for the GEO ID: GSE28847  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM714355 | GPL570 |
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PASMC_0h
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PASMCs without BMP stimulation
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tissue: pulmonary arterial smooth muscle cells (PASMCs)
treatment: none
treatment duration: 0 hrs
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| Sample_geo_accession | GSM714355
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Apr 25 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with 50 ng/ml BMP-4 for the indicated times.
| | Sample_growth_protocol_ch1 | PASMCs were maintained in SmGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using the RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM714nnn/GSM714355/suppl/GSM714355.CEL.gz
| | Sample_series_id | GSE27661
| | Sample_series_id | GSE28847
| | Sample_data_row_count | 54675
| | |
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GSM714356 | GPL570 |
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PASMC_BMP4_2h
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PASMCs stimulated with BMP-4 for 2 h
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tissue: pulmonary arterial smooth muscle cells (PASMCs)
treatment: BMP-4
treatment duration: 2 hrs
|
|
| Sample_geo_accession | GSM714356
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Apr 25 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with 50 ng/ml BMP-4 for the indicated times.
| | Sample_growth_protocol_ch1 | PASMCs were maintained in SmGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using the RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM714nnn/GSM714356/suppl/GSM714356.CEL.gz
| | Sample_series_id | GSE27661
| | Sample_series_id | GSE28847
| | Sample_data_row_count | 54675
| | |
|
GSM714357 | GPL570 |
|
PASMC_BMP4_24h
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PASMCs stimulated with BMP-4 for 24 h
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tissue: pulmonary arterial smooth muscle cells (PASMCs)
treatment: BMP-4
treatment duration: 24 hrs
|
|
| Sample_geo_accession | GSM714357
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Apr 25 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with 50 ng/ml BMP-4 for the indicated times.
| | Sample_growth_protocol_ch1 | PASMCs were maintained in SmGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using the RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al., Genomics 68:136-143, 2000 [PMID 10964511]).
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM714nnn/GSM714357/suppl/GSM714357.CEL.gz
| | Sample_series_id | GSE27661
| | Sample_series_id | GSE28847
| | Sample_data_row_count | 54675
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