Search results for the GEO ID: GSE28896 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM716064 | GPL3921 |
|
CD34+ cells treated with Dexamethasone, rep1
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone
|
DEX.1
|
Sample_geo_accession | GSM716064
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716064/suppl/GSM716064.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716065 | GPL3921 |
|
CD34+ cells treated with Dexamethasone, rep2
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone
|
DEX.2
|
Sample_geo_accession | GSM716065
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716065/suppl/GSM716065.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716066 | GPL3921 |
|
CD34+ cells treated with Dexamethasone, rep3
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone
|
DEX.3
|
Sample_geo_accession | GSM716066
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716066/suppl/GSM716066.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716067 | GPL3921 |
|
CD34+ cells treated with Lenalidomide, rep1
|
Cryopreserved human bone marrow CD34+ cells, lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: lenalidomide
|
LEN.1
|
Sample_geo_accession | GSM716067
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716067/suppl/GSM716067.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716068 | GPL3921 |
|
CD34+ cells treated with Lenalidomide, rep2
|
Cryopreserved human bone marrow CD34+ cells, lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: lenalidomide
|
LEN.2
|
Sample_geo_accession | GSM716068
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716068/suppl/GSM716068.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716069 | GPL3921 |
|
CD34+ cells treated with Lenalidomide, rep3
|
Cryopreserved human bone marrow CD34+ cells, lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: lenalidomide
|
LEN.3
|
Sample_geo_accession | GSM716069
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716069/suppl/GSM716069.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716070 | GPL3921 |
|
CD34+ cells treated with Dexamethasone and Lenalidomide, rep1
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone and lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone and lenalidomide
|
DEX+LEN.1
|
Sample_geo_accession | GSM716070
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716070/suppl/GSM716070.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716071 | GPL3921 |
|
CD34+ cells treated with Dexamethasone and Lenalidomide, rep2
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone and lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone and lenalidomide
|
DEX+LEN.2
|
Sample_geo_accession | GSM716071
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716071/suppl/GSM716071.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716072 | GPL3921 |
|
CD34+ cells treated with Dexamethasone and Lenalidomide, rep3
|
Cryopreserved human bone marrow CD34+ cells, dexamethasone and lenalidomide
|
cell type: bone marrow CD34+ cells
treatment: dexamethasone and lenalidomide
|
DEX+LEN.3
|
Sample_geo_accession | GSM716072
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716072/suppl/GSM716072.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716073 | GPL3921 |
|
Untreated CD34+ cells, rep1
|
Cryopreserved human bone marrow CD34+ cells, untreated
|
cell type: bone marrow CD34+ cells
treatment: none
|
UNTREATED.1
|
Sample_geo_accession | GSM716073
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716073/suppl/GSM716073.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716074 | GPL3921 |
|
Untreated CD34+ cells, rep2
|
Cryopreserved human bone marrow CD34+ cells, untreated
|
cell type: bone marrow CD34+ cells
treatment: none
|
UNTREATED.2
|
Sample_geo_accession | GSM716074
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716074/suppl/GSM716074.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
|
GSM716075 | GPL3921 |
|
Untreated CD34+ cells, rep3
|
Cryopreserved human bone marrow CD34+ cells, untreated
|
cell type: bone marrow CD34+ cells
treatment: none
|
UNTREATED.3
|
Sample_geo_accession | GSM716075
| Sample_status | Public on Apr 28 2011
| Sample_submission_date | Apr 27 2011
| Sample_last_update_date | Apr 28 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were cultured in the presence of dexamethasone and lenalidomide, individually and in combination, for 24 hours.
| Sample_growth_protocol_ch1 | Human CD34+ cells were cultured for 48 hours in the erythroid differentiation media described above with the addition of 40 ng/mL of FMS-like tyrosine kinase 3 (flt-3; Miltenyi Biotech) and 15 ng/mL of Granulocyte colony-stimulating factor (G-CSF; Amgen).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was purified using Trizol (Invitrogen). The RNA was amplified using a linear amplification protocol (Nugen Ovation V2 amplification system).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | NuGen Ovation Biotin labeling system.
| Sample_hyb_protocol | Standard Affymetrix protocol.
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | Signal normalization was performed by the RMA method.
| Sample_platform_id | GPL3921
| Sample_contact_name | Fatima,,Al-Shahrour
| Sample_contact_email | shahrour@broadinstitute.org
| Sample_contact_department | Cancer program
| Sample_contact_institute | Broad Institute of MIT and Harvard
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02142
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM716nnn/GSM716075/suppl/GSM716075.CEL.gz
| Sample_series_id | GSE28896
| Sample_data_row_count | 22277
| |
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