Search results for the GEO ID: GSE29045 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM719761 | GPL1261 |
|
1-ABB2
|
ABB2
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5a-/-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719761
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719761/suppl/GSM719761.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719762 | GPL1261 |
|
2-ABB3
|
ABB3
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5a-/-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719762
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719762/suppl/GSM719762.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719763 | GPL1261 |
|
3-ABB4
|
ABB4
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5a-/-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719763
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719763/suppl/GSM719763.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719764 | GPL1261 |
|
4-AAB6
|
AAB6
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5ab+/null
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719764
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719764/suppl/GSM719764.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719765 | GPL1261 |
|
5-AAB7
|
AAB7
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5ab+/null
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719765
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719765/suppl/GSM719765.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719766 | GPL1261 |
|
6-AAB8
|
AAB8
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5ab+/null
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719766
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719766/suppl/GSM719766.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719767 | GPL1261 |
|
7_83
|
83
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5b-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719767
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719767/suppl/GSM719767.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719768 | GPL1261 |
|
8_909
|
909
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5b-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719768
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719768/suppl/GSM719768.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719769 | GPL1261 |
|
9_913
|
913
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5b-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719769
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719769/suppl/GSM719769.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719770 | GPL1261 |
|
10_957
|
957
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5a-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719770
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719770/suppl/GSM719770.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
| |
|
GSM719771 | GPL1261 |
|
11_958
|
958
|
background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5a-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719771
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719771/suppl/GSM719771.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
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GSM719772 | GPL1261 |
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12_959
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959
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background strain: mixed background of C57BL/6, FVB, and 129
genotype/variation: Stat5abnull/Stat5a-
|
The sorted cell subpopulations
|
Sample_geo_accession | GSM719772
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 04 2011
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The Mx1-Cre transgene was activated by intraperitoneal injection of PolyIC (polyinosinic: polycytidylic acid) as described (Kimura et al., 2009). To obtain hematologic data, peripheral blood was collected in heparinized capillary tubes (Drummond Scientific Co., Broomall, PA) by retro-orbital puncture and complete blood counts (CBCs) were determined with HEMAVET HV950FS (Drew Scientific, Dallas, TX).
| Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | RMA by software Partek
| Sample_platform_id | GPL1261
| Sample_contact_name | WeiPing,,Chen
| Sample_contact_email | weipingChen@niddk.nih.gov
| Sample_contact_phone | 301-496-0175
| Sample_contact_laboratory | Microarray Core Lab
| Sample_contact_department | MCL
| Sample_contact_institute | NIDDK/NIH
| Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM719nnn/GSM719772/suppl/GSM719772.CEL.gz
| Sample_series_id | GSE29045
| Sample_data_row_count | 45101
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