Search results for the GEO ID: GSE29083  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM720604 | GPL1261 |
|
1-WT
|
WT
|
genotype/variation: wild type
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720604
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720604/suppl/GSM720604.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720605 | GPL1261 |
|
2-WT
|
WT
|
genotype/variation: wild type
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720605
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720605/suppl/GSM720605.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720606 | GPL1261 |
|
3-WT
|
WT
|
genotype/variation: wild type
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720606
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720606/suppl/GSM720606.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720607 | GPL1261 |
|
4-KO
|
Gb5 KO
|
genotype/variation: Gb5-deficient
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720607
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720607/suppl/GSM720607.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720608 | GPL1261 |
|
5-KO
|
Gb5 KO
|
genotype/variation: Gb5-deficient
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720608
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720608/suppl/GSM720608.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720609 | GPL1261 |
|
6-KO
|
Gb5 KO
|
genotype/variation: Gb5-deficient
tissue: Brain Tissues
|
|
| Sample_geo_accession | GSM720609
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720609/suppl/GSM720609.CEL.gz
| | Sample_series_id | GSE29081
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720610 | GPL1261 |
|
1-Gb5ko
|
1-Gb5ko
|
genotype/variation: Gb5-deficient
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720610
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720610/suppl/GSM720610.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720611 | GPL1261 |
|
2-Gb5ko
|
2-Gb5ko
|
genotype/variation: Gb5-deficient
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720611
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720611/suppl/GSM720611.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720612 | GPL1261 |
|
3-Gb5ko
|
3-Gb5ko
|
genotype/variation: Gb5-deficient
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720612
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720612/suppl/GSM720612.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720613 | GPL1261 |
|
4-Gb5wt
|
4-Gb5wt
|
genotype/variation: wild type
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720613
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720613/suppl/GSM720613.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
| | |
|
GSM720614 | GPL1261 |
|
5-Gb5wt
|
5-Gb5wt
|
genotype/variation: wild type
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720614
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720614/suppl/GSM720614.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
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GSM720615 | GPL1261 |
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6-Gb5wt
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6-Gb5wt
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genotype/variation: wild type
tissue: non-cerebellar portion of brain
|
|
| Sample_geo_accession | GSM720615
| | Sample_status | Public on Sep 13 2011
| | Sample_submission_date | May 05 2011
| | Sample_last_update_date | Sep 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
| | Sample_growth_protocol_ch1 | All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA by software Partek
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM720nnn/GSM720615/suppl/GSM720615.CEL.gz
| | Sample_series_id | GSE29082
| | Sample_series_id | GSE29083
| | Sample_data_row_count | 45101
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