Search results for the GEO ID: GSE29112  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM721025 | GPL1261 |
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Runx1-/VE-cadherin+/Flk1+, expression
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FACS-sorted day 6 differentiated ES cells, Runx1-/VE-cadherin+/Flk1+
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cell line: Runx1(Venus/+) ES cell line
cell type: differentiated ES cells
days of differentiation: 6
cell subpopulation: Runx1-/VE-cadherin+/Flk1+
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Gene expression data from FACs-sorted day 6 differentiated ES cells.
Runx1(Venus/+) ES cell line: The Venus gene was placed in the P2-5'UTR under the control of the Runx1 proximal promoter P2. Parental ES cells are CCE ES cells.
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| Sample_geo_accession | GSM721025
| | Sample_status | Public on May 29 2012
| | Sample_submission_date | May 06 2011
| | Sample_last_update_date | May 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment beyond the normal growth protocol was used.
| | Sample_growth_protocol_ch1 | 1 x 10^5 undifferentiated ES cells were cultured on confluent OP9 cell layers in 10-cm dishes to induce differentiation. After 6 days of ES cell differentiation, cultured cells were harvested for FACS analysis and sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using the QIAGEN RNeasy Micro kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The 3'IVT Express Kit was used for biotin labeling according to the manufacturer's instructions (Affymetrix).
| | Sample_hyb_protocol | RNA was subsequently fragmented and hybridized to the GeneChip according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were processed using MAS5.0 (Affymetrix GCOS Ver.1.3).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Anagha,M,Joshi
| | Sample_contact_email | aj379@cam.ac.uk
| | Sample_contact_phone | +44 01223 336822
| | Sample_contact_laboratory | Dr. Gottgens
| | Sample_contact_department | Dept. of haematology, CIMR
| | Sample_contact_institute | University of cambridge
| | Sample_contact_address | Wellcome trust/MRC building, Hills road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB20XY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721025/suppl/GSM721025.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721025/suppl/GSM721025.CHP.gz
| | Sample_series_id | GSE29112
| | Sample_series_id | GSE29515
| | Sample_data_row_count | 45101
| | |
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GSM721026 | GPL1261 |
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Runx1+/VE-cadherin+/CD41+, expression
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FACS-sorted day 6 differentiated ES cells, Runx1+/VE-cadherin+/CD41+
|
cell line: Runx1(Venus/+) ES cell line
cell type: differentiated ES cells
days of differentiation: 6
cell subpopulation: Runx1+/VE-cadherin+/CD41+
|
Gene expression data from FACs-sorted day 6 differentiated ES cells.
Runx1(Venus/+) ES cell line: The Venus gene was placed in the P2-5'UTR under the control of the Runx1 proximal promoter P2. Parental ES cells are CCE ES cells.
|
| Sample_geo_accession | GSM721026
| | Sample_status | Public on May 29 2012
| | Sample_submission_date | May 06 2011
| | Sample_last_update_date | May 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment beyond the normal growth protocol was used.
| | Sample_growth_protocol_ch1 | 1 x 10^5 undifferentiated ES cells were cultured on confluent OP9 cell layers in 10-cm dishes to induce differentiation. After 6 days of ES cell differentiation, cultured cells were harvested for FACS analysis and sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using the QIAGEN RNeasy Micro kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The 3'IVT Express Kit was used for biotin labeling according to the manufacturer's instructions (Affymetrix).
| | Sample_hyb_protocol | RNA was subsequently fragmented and hybridized to the GeneChip according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were processed using MAS5.0 (Affymetrix GCOS Ver.1.3).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Anagha,M,Joshi
| | Sample_contact_email | aj379@cam.ac.uk
| | Sample_contact_phone | +44 01223 336822
| | Sample_contact_laboratory | Dr. Gottgens
| | Sample_contact_department | Dept. of haematology, CIMR
| | Sample_contact_institute | University of cambridge
| | Sample_contact_address | Wellcome trust/MRC building, Hills road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB20XY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721026/suppl/GSM721026.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721026/suppl/GSM721026.CHP.gz
| | Sample_series_id | GSE29112
| | Sample_series_id | GSE29515
| | Sample_data_row_count | 45101
| | |
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GSM721027 | GPL1261 |
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Runx1+/VE-cadherin+/CD41-, expression
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FACS-sorted day 6 differentiated ES cells, Runx1+/VE-cadherin+/CD41-
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cell line: Runx1(Venus/+) ES cell line
cell type: differentiated ES cells
days of differentiation: 6
cell subpopulation: Runx1+/VE-cadherin+/CD41-
|
Gene expression data from FACs-sorted day 6 differentiated ES cells.
Runx1(Venus/+) ES cell line: The Venus gene was placed in the P2-5'UTR under the control of the Runx1 proximal promoter P2. Parental ES cells are CCE ES cells.
|
| Sample_geo_accession | GSM721027
| | Sample_status | Public on May 29 2012
| | Sample_submission_date | May 06 2011
| | Sample_last_update_date | May 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment beyond the normal growth protocol was used.
| | Sample_growth_protocol_ch1 | 1 x 10^5 undifferentiated ES cells were cultured on confluent OP9 cell layers in 10-cm dishes to induce differentiation. After 6 days of ES cell differentiation, cultured cells were harvested for FACS analysis and sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using the QIAGEN RNeasy Micro kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The 3'IVT Express Kit was used for biotin labeling according to the manufacturer's instructions (Affymetrix).
| | Sample_hyb_protocol | RNA was subsequently fragmented and hybridized to the GeneChip according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were processed using MAS5.0 (Affymetrix GCOS Ver.1.3).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Anagha,M,Joshi
| | Sample_contact_email | aj379@cam.ac.uk
| | Sample_contact_phone | +44 01223 336822
| | Sample_contact_laboratory | Dr. Gottgens
| | Sample_contact_department | Dept. of haematology, CIMR
| | Sample_contact_institute | University of cambridge
| | Sample_contact_address | Wellcome trust/MRC building, Hills road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB20XY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721027/suppl/GSM721027.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721027/suppl/GSM721027.CHP.gz
| | Sample_series_id | GSE29112
| | Sample_series_id | GSE29515
| | Sample_data_row_count | 45101
| | |
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GSM721028 | GPL1261 |
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Runx1+/VE-cadherin-/CD41+, expression
|
FACS-sorted day 6 differentiated ES cells, Runx1+/VE-cadherin-/CD41+
|
cell line: Runx1(Venus/+) ES cell line
cell type: differentiated ES cells
days of differentiation: 6
cell subpopulation: Runx1+/VE-cadherin-/CD41+
|
Gene expression data from FACs-sorted day 6 differentiated ES cells.
Runx1(Venus/+) ES cell line: The Venus gene was placed in the P2-5'UTR under the control of the Runx1 proximal promoter P2. Parental ES cells are CCE ES cells.
|
| Sample_geo_accession | GSM721028
| | Sample_status | Public on May 29 2012
| | Sample_submission_date | May 06 2011
| | Sample_last_update_date | May 29 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment beyond the normal growth protocol was used.
| | Sample_growth_protocol_ch1 | 1 x 10^5 undifferentiated ES cells were cultured on confluent OP9 cell layers in 10-cm dishes to induce differentiation. After 6 days of ES cell differentiation, cultured cells were harvested for FACS analysis and sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed by using the QIAGEN RNeasy Micro kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The 3'IVT Express Kit was used for biotin labeling according to the manufacturer's instructions (Affymetrix).
| | Sample_hyb_protocol | RNA was subsequently fragmented and hybridized to the GeneChip according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were processed using MAS5.0 (Affymetrix GCOS Ver.1.3).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Anagha,M,Joshi
| | Sample_contact_email | aj379@cam.ac.uk
| | Sample_contact_phone | +44 01223 336822
| | Sample_contact_laboratory | Dr. Gottgens
| | Sample_contact_department | Dept. of haematology, CIMR
| | Sample_contact_institute | University of cambridge
| | Sample_contact_address | Wellcome trust/MRC building, Hills road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB20XY
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721028/suppl/GSM721028.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721028/suppl/GSM721028.CHP.gz
| | Sample_series_id | GSE29112
| | Sample_series_id | GSE29515
| | Sample_data_row_count | 45101
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