Result

Search results for the GEO ID: GSE29115

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Description

Characteristics

GSM721064

GPL570

H9 undiff. unsorted. Day0, biological rep1

H9 Day 0

cell line: H9 developmental stage: Undifferentiated

Gene expression data from undifferentied human embryonic stem cells

Sample_geo_accession	GSM721064
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721064/suppl/GSM721064.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721065

GPL570

H9 undiff. unsorted. Day0, biological rep2

H9 Day 0

cell line: H9 developmental stage: Undifferentiated

Gene expression data from undifferentied human embryonic stem cells

Sample_geo_accession	GSM721065
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721065/suppl/GSM721065.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721066

GPL570

H9 diff. KDR+CD31+sorted. Day4, biological rep1

H9 KDR+CD31+ Day 4

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 4 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 4 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721066
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721066/suppl/GSM721066.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721067

GPL570

H9 diff. KDR+CD31+sorted. Day4, biological rep2

H9 KDR+CD31+ Day 4

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 4 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 4 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721067
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721067/suppl/GSM721067.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721068

GPL570

H9 diff. KDR+CD31+sorted. Day6, biological rep1

H9 KDR+CD31+ Day 6

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 6 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 6 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721068
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721068/suppl/GSM721068.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721069

GPL570

H9 diff. KDR+CD31+sorted. Day6, biological rep2

H9 KDR+CD31+ Day 4

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 6 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 6 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721069
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721069/suppl/GSM721069.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721070

GPL570

H9 diff. KDR+CD31+sorted. Day8, biological rep1

H9 KDR+CD31+ Day 4

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 8 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 8 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721070
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721070/suppl/GSM721070.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

GSM721071

GPL570

H9 diff. KDR+CD31+sorted. Day8, biological rep2

H9 KDR+CD31+ Day 4

cell line: H9 cell type: FACS sorted KDR+CD31 developmental stage: Day 8 of Differentiation

Gene expression data from KDR+CD31+ FACS sorted cells from 8 days of haematopoietic differentiation of human embryonic stem cells

Sample_geo_accession	GSM721071
Sample_status	Public on Jan 06 2012
Sample_submission_date	May 06 2011
Sample_last_update_date	Jan 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Differentiation of human embryonic stem cells within optimised haematopoietic media consisting of StemPro34 Serum-free media (Invitrogen), Penicillin-Streptomycin Solution (1mM), Bone Morphogenic Protein 4 (BMP-4), Glutamax (2mM), Monothioglycerol (MTG),
Sample_growth_protocol_ch1	The human embryonic stem cell lines H9 and H1 (WiCell. Inc.) were routinely passaged and maintained in human embryonic stem cell media on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layers and differentiation was achieved by forming em
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Sun,,Yung
Sample_contact_email	sun.yung@ncl.ac.uk
Sample_contact_laboratory	Stem Cell
Sample_contact_department	Institute of Genetic Medicine
Sample_contact_institute	Newcastle University
Sample_contact_address	Central Parkway
Sample_contact_city	Newcastle upon Tyne
Sample_contact_zip/postal_code	NE1 3BZ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721071/suppl/GSM721071.CEL.gz
Sample_series_id	GSE29115
Sample_data_row_count	54675

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