Search results for the GEO ID: GSE29137  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM721316 | GPL570 |
|
MCF7-GFP, Biological Rep 1
|
breast cell
|
cell line: MCF7
transfection: GFP vector control
|
0_Cheng_GFP1_012209.CHP
0_Cheng_GFP1_012209.CEL
|
| Sample_geo_accession | GSM721316
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721316/suppl/GSM721316.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721316/suppl/GSM721316.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721317 | GPL570 |
|
MCF7-GFP, Biological Rep 2
|
breast cell
|
cell line: MCF7
transfection: GFP vector control
|
0_Cheng_GFP2_012209.CHP
0_Cheng_GFP2_012209.CEL
|
| Sample_geo_accession | GSM721317
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721317/suppl/GSM721317.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721317/suppl/GSM721317.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721318 | GPL570 |
|
MCF7-GFP, Biological Rep 3
|
breast cell
|
cell line: MCF7
transfection: GFP vector control
|
0_Cheng_GFP3_012209.CHP
0_Cheng_GFP3_012209.CEL
|
| Sample_geo_accession | GSM721318
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721318/suppl/GSM721318.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721318/suppl/GSM721318.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721319 | GPL570 |
|
MCF7-Numb4, Biological Rep 1
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb4 Transfected
|
0_Cheng_N41_012209.CHP
0_Cheng_N41_012209.CEL
|
| Sample_geo_accession | GSM721319
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721319/suppl/GSM721319.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721319/suppl/GSM721319.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721320 | GPL570 |
|
MCF7-Numb4, Biological Rep 2
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb4 Transfected
|
0_Cheng_N42_012209.CHP
0_Cheng_N42_012209.CEL
|
| Sample_geo_accession | GSM721320
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721320/suppl/GSM721320.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721320/suppl/GSM721320.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721321 | GPL570 |
|
MCF7-Numb4, Biological Rep 3
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb4 Transfected
|
0_Cheng_N43_012209.CHP
0_Cheng_N43_012209.CEL
|
| Sample_geo_accession | GSM721321
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721321/suppl/GSM721321.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721321/suppl/GSM721321.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721322 | GPL570 |
|
MCF7-Numb5, Biological Rep 1
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb5 Transfected
|
0_Cheng_N51_012209.CHP
0_Cheng_N51_012209.CEL
|
| Sample_geo_accession | GSM721322
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721322/suppl/GSM721322.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721322/suppl/GSM721322.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721323 | GPL570 |
|
MCF7-Numb5, Biological Rep 2
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb5 Transfected
|
0_Cheng_N52_012209.CHP
0_Cheng_N52_012209.CEL
|
| Sample_geo_accession | GSM721323
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721323/suppl/GSM721323.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721323/suppl/GSM721323.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721324 | GPL570 |
|
MCF7-Numb5, Biological Rep 3
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb5 Transfected
|
0_Cheng_N53_012209.CHP
0_Cheng_N53_012209.CEL
|
| Sample_geo_accession | GSM721324
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721324/suppl/GSM721324.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721324/suppl/GSM721324.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721325 | GPL570 |
|
MCF7-Numb6, Biological Rep 1
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb6 Transfected
|
0_Cheng_N61_012209.CHP
0_Cheng_N61_012209.CEL
|
| Sample_geo_accession | GSM721325
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721325/suppl/GSM721325.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721325/suppl/GSM721325.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721326 | GPL570 |
|
MCF7-Numb6, Biological Rep 2
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb6 Transfected
|
0_Cheng_N62_012209.CHP
0_Cheng_N62_012209.CEL
|
| Sample_geo_accession | GSM721326
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721326/suppl/GSM721326.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721326/suppl/GSM721326.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721327 | GPL570 |
|
MCF7-Numb6, Biological Rep 3
|
breast cell
|
cell line: MCF7
transfection: GFP-Numb6 Transfected
|
0_Cheng_N63_012209.CHP
0_Cheng_N63_012209.CEL
|
| Sample_geo_accession | GSM721327
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721327/suppl/GSM721327.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721327/suppl/GSM721327.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721328 | GPL570 |
|
MB231-GFP, Biological Rep 1
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP vector control
|
0_Cheng_MB-GFP1_111808.CHP
0_Cheng_MB-GFP1_111808.CEL
|
| Sample_geo_accession | GSM721328
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721328/suppl/GSM721328.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721328/suppl/GSM721328.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721329 | GPL570 |
|
MB231-GFP, Biological Rep 2
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP vector control
|
0_Cheng_MB-GFP2_111808.CHP
0_Cheng_MB-GFP2_111808.CEL
|
| Sample_geo_accession | GSM721329
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721329/suppl/GSM721329.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721329/suppl/GSM721329.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721330 | GPL570 |
|
MB231-GFP, Biological Rep 3
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP vector control
|
0_Cheng_MB-GFP3_111808.CHP
0_Cheng_MB-GFP3_111808.CEL
|
| Sample_geo_accession | GSM721330
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721330/suppl/GSM721330.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721330/suppl/GSM721330.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721331 | GPL570 |
|
MB231-Numb4, Biological Rep 1
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb4 Transfected
|
0_Cheng_MB41_111808.CHP
0_Cheng_MB41_111808.CEL
|
| Sample_geo_accession | GSM721331
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721331/suppl/GSM721331.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721331/suppl/GSM721331.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721332 | GPL570 |
|
MB231-Numb4, Biological Rep 2
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb4 Transfected
|
0_Cheng_MB42_111808.CHP
0_Cheng_MB42_111808.CEL
|
| Sample_geo_accession | GSM721332
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721332/suppl/GSM721332.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721332/suppl/GSM721332.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721333 | GPL570 |
|
MB231-Numb4, Biological Rep 3
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb4 Transfected
|
0_Cheng_MB43_111808.CHP
0_Cheng_MB43_111808.CEL
|
| Sample_geo_accession | GSM721333
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721333/suppl/GSM721333.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721333/suppl/GSM721333.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721334 | GPL570 |
|
MB231-Numb5, Biological Rep 1
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb5 Transfected
|
0_Cheng_MB51_111808.CHP
0_Cheng_MB51_111808.CEL
|
| Sample_geo_accession | GSM721334
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721334/suppl/GSM721334.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721334/suppl/GSM721334.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721335 | GPL570 |
|
MB231-Numb5, Biological Rep 2
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb5 Transfected
|
0_Cheng_MB52_111808.CHP
0_Cheng_MB52_111808.CEL
|
| Sample_geo_accession | GSM721335
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721335/suppl/GSM721335.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721335/suppl/GSM721335.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721336 | GPL570 |
|
MB231-Numb5, Biological Rep 3
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb5 Transfected
|
0_Cheng_MB53_111808.CHP
0_Cheng_MB53_111808.CEL
|
| Sample_geo_accession | GSM721336
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721336/suppl/GSM721336.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721336/suppl/GSM721336.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721337 | GPL570 |
|
MB231-Numb6, Biological Rep 1
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb6 Transfected
|
0_Cheng_MB61_111808.CHP
0_Cheng_MB61_111808.CEL
|
| Sample_geo_accession | GSM721337
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721337/suppl/GSM721337.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721337/suppl/GSM721337.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721338 | GPL570 |
|
MB231-Numb6, Biological Rep 2
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb6 Transfected
|
0_Cheng_MB62_111808.CHP
0_Cheng_MB62_111808.CEL
|
| Sample_geo_accession | GSM721338
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721338/suppl/GSM721338.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721338/suppl/GSM721338.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
GSM721339 | GPL570 |
|
MB231-Numb6, Biological Rep 3
|
breast cell
|
cell line: MDA-MB-231
transfection: GFP-Numb6 Transfected
|
0_Cheng_MB63_111808.CHP
0_Cheng_MB63_111808.CEL
|
| Sample_geo_accession | GSM721339
| | Sample_status | Public on May 09 2011
| | Sample_submission_date | May 09 2011
| | Sample_last_update_date | May 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were transfected with CMV-GFP vector inserted with null, Numb4, Numb5 or Numb6 plasmid.
| | Sample_growth_protocol_ch1 | MCF7 cells were cultured withDMEM/F12, plus 10% FBS and 0.01 mg/ml bovine insulin. MDA-MB-231 cells were cultured with alpha modified MEM medium plus 10% FBS. Both cells were add 1% penicollin/streptomycin. Both cells with stably transfections were added 0.5 mg/ml of G418. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with Trizol. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix Genomic Command Console using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Cheng
| | Sample_contact_email | Robert.Cheng2@nih.gov
| | Sample_contact_phone | 301-402-2014
| | Sample_contact_laboratory | RBB
| | Sample_contact_department | CCR
| | Sample_contact_institute | NCI
| | Sample_contact_address | 10 Center Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721339/suppl/GSM721339.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721339/suppl/GSM721339.CHP.gz
| | Sample_series_id | GSE29137
| | Sample_data_row_count | 54675
| | |
|
| |
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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