Search results for the GEO ID: GSE29232  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM722972 | GPL570 |
|
Control RWPE-1-AR cells at 3h, biological rep1
|
Control RWPE-1-AR cells at 3h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 3h (control).
|
| Sample_geo_accession | GSM722972
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722972/suppl/GSM722972_Allio1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722972/suppl/GSM722972_Allio1.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722973 | GPL570 |
|
Control RWPE-1-AR cells at 3h, biological rep2
|
Control RWPE-1-AR cells at 3h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 3h (control).
|
| Sample_geo_accession | GSM722973
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722973/suppl/GSM722973_Allio2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722973/suppl/GSM722973_Allio2.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722974 | GPL570 |
|
Control RWPE-1-AR cells at 3h, biological rep3
|
Control RWPE-1-AR cells at 3h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 3h (control).
|
| Sample_geo_accession | GSM722974
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722974/suppl/GSM722974_Allio3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722974/suppl/GSM722974_Allio3.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722975 | GPL570 |
|
R1881-treated 3h RWPE-1-AR cells, biological rep1
|
3h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 3h
|
| Sample_geo_accession | GSM722975
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722975/suppl/GSM722975_Allio4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722975/suppl/GSM722975_Allio4.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722976 | GPL570 |
|
R1881-treated 3h RWPE-1-AR cells, biological rep2
|
3h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 3h
|
| Sample_geo_accession | GSM722976
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722976/suppl/GSM722976_Allio5.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722976/suppl/GSM722976_Allio5.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722977 | GPL570 |
|
R1881-treated 3h RWPE-1-AR cells, biological rep3
|
3h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 3h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 3h
|
| Sample_geo_accession | GSM722977
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722977/suppl/GSM722977_Allio6.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722977/suppl/GSM722977_Allio6.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722978 | GPL570 |
|
Control RWPE-1-AR cells at 24h, biological rep1
|
Control RWPE-1-AR cells at 24h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 24h (control).
|
| Sample_geo_accession | GSM722978
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722978/suppl/GSM722978_Allio7.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722978/suppl/GSM722978_Allio7.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722979 | GPL570 |
|
Control RWPE-1-AR cells at 24h, biological rep2
|
Control RWPE-1-AR cells at 24h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 24h (control).
|
| Sample_geo_accession | GSM722979
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722979/suppl/GSM722979_Allio8.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722979/suppl/GSM722979_Allio8.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722980 | GPL570 |
|
Control RWPE-1-AR cells at 24h, biological rep3
|
Control RWPE-1-AR cells at 24h
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with vehicle (ethanol) for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih vehicle for 24h (control).
|
| Sample_geo_accession | GSM722980
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722980/suppl/GSM722980_Allio9.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722980/suppl/GSM722980_Allio9.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722981 | GPL570 |
|
R1881-treated 24h RWPE-1-AR cells, biological rep1
|
24h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 24h
|
| Sample_geo_accession | GSM722981
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722981/suppl/GSM722981_Allio10.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722981/suppl/GSM722981_Allio10.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722982 | GPL570 |
|
R1881-treated 24h RWPE-1-AR cells, biological rep2
|
24h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 24h
|
| Sample_geo_accession | GSM722982
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722982/suppl/GSM722982_Allio11.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722982/suppl/GSM722982_Allio11.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
|
GSM722983 | GPL570 |
|
R1881-treated 24h RWPE-1-AR cells, biological rep3
|
24h 1nM R1881-treated RWPE-1-AR cells
|
celll line: RWPE-1-AR
treatment protocol: RWPE-1-AR prostatic cell line was steroid-depleted for 48h and treated with 1nM R1881 for 24h
|
Gene expression data from RWPE-1-AR cell line treated wih R1881for 24h
|
| Sample_geo_accession | GSM722983
| | Sample_status | Public on May 12 2011
| | Sample_submission_date | May 11 2011
| | Sample_last_update_date | May 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RWPE-1-AR cells were stimulated with a non-metabolisable androgen, R1881, in the growth medium deprived of BPE for 48h.
| | Sample_growth_protocol_ch1 | RWPE-1-AR cells were maintained in keratinocyte growth medium (Invitrogen 17005-042) supplemented with rEGF (recombinant epithelial growth factor) and BPE (bovine pituary extract) (Invitrogen 37000015) at 37°C, 5%CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted using Rneasy mini kit (Qiagen) following manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
| | Sample_hyb_protocol | 15 ug of cRNA were hybridized according to the Affymetrix protocol (Expression Analysis Technical Manual, 2008, Affymetrix) using the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Virginie,,Vlaeminck-Guillem
| | Sample_contact_email | virginie.vlaeminck-guillem@univ-lyon1.fr
| | Sample_contact_phone | 33 4 26 23 59 34
| | Sample_contact_fax | 33 4 26 23 59 00
| | Sample_contact_laboratory | Génomique Fonctionnelle des Récepteurs Nucléaires UMR5242CNRS
| | Sample_contact_department | Faculté de Médecine Lyon Sud
| | Sample_contact_institute | Institut de Génomique Fonctionnelle de Lyon
| | Sample_contact_address | Chemin du Grand Revoyet
| | Sample_contact_city | OULLINS
| | Sample_contact_zip/postal_code | 69600
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722983/suppl/GSM722983_Allio12.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM722nnn/GSM722983/suppl/GSM722983_Allio12.CHP.gz
| | Sample_series_id | GSE29232
| | Sample_data_row_count | 54675
| | |
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