Search results for the GEO ID: GSE29313  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM724436 | GPL570 |
|
Cytosol_Extract_1
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Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: none
fraction: Cytosol_Extract
|
Input RNA from cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep1
|
| Sample_geo_accession | GSM724436
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724436/suppl/GSM724436.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724437 | GPL570 |
|
Cytosol_Extract_2
|
Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: none
fraction: Cytosol_Extract
|
Input RNA from cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep2
|
| Sample_geo_accession | GSM724437
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724437/suppl/GSM724437.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724438 | GPL570 |
|
Cytosol_Extract_3
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Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: none
fraction: Cytosol_Extract
|
Input RNA from cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep3
|
| Sample_geo_accession | GSM724438
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724438/suppl/GSM724438.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724439 | GPL570 |
|
IP_1
|
Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: anti-FLAG M2
rip antibody vendor: Sigma
rip antibody lot#: 037K6001
rip antibody catalog#: A2220
fraction: IP
|
RNA precipitated with anti-FLAG M2 affinity gel from the cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep1
|
| Sample_geo_accession | GSM724439
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724439/suppl/GSM724439.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724440 | GPL570 |
|
IP_2
|
Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: anti-FLAG M2
rip antibody vendor: Sigma
rip antibody lot#: 037K6001
rip antibody catalog#: A2220
fraction: IP
|
RNA precipitated with anti-FLAG M2 affinity gel from the cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep2
|
| Sample_geo_accession | GSM724440
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724440/suppl/GSM724440.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724441 | GPL570 |
|
IP_3
|
Cytosol extract of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: anti-FLAG M2
rip antibody vendor: Sigma
rip antibody lot#: 037K6001
rip antibody catalog#: A2220
fraction: IP
|
RNA precipitated with anti-FLAG M2 affinity gel from the cytosol extract of HeLa S3 cells transfected with pFLAG-EWS, biological rep3
|
| Sample_geo_accession | GSM724441
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724441/suppl/GSM724441.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724442 | GPL570 |
|
Whole_Cell_Lysate
|
Whole cell lysate of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: none
fraction: Whole_Cell_Lysate
|
Reference RNA from whole cell extract of HeLa S3 cells transfected with pFLAG-EWS
|
| Sample_geo_accession | GSM724442
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724442/suppl/GSM724442.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
|
GSM724443 | GPL570 |
|
Whole_Cell_Lysate_IP
|
Whole cell lysate of HeLa S3 cells transfected with pFLAG-EWS
|
cell line: Human cervical cancer cell line HeLa S3
rip antibody: anti-FLAG M2
rip antibody vendor: Sigma
rip antibody lot#: 037K6001
rip antibody catalog#: A2220
fraction: Whole_Cell_Lysate_IP
|
Reference RNA precipitated with anti-FLAG M2 affinity gel from whole cell extract of HeLa S3 cells transfected with pFLAG-EWS
|
| Sample_geo_accession | GSM724443
| | Sample_status | Public on Jan 16 2012
| | Sample_submission_date | May 16 2011
| | Sample_last_update_date | Jan 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cells were transfected with pFLAG-EWS and incubated for 48 h.
| | Sample_growth_protocol_ch1 | Human cervical cancer cell line HeLa S3 was grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% calf serum in a humidified atmosphere containing 5% CO2 at 37° C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRI reagent (Sigma).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | CEL files were quantified with Factor for Robust Microarray Summarization (FARMS), using the statistical language R and Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yuji,,Nakai
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | 1-1-1, Yayoi
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-8657
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM724nnn/GSM724443/suppl/GSM724443.CEL.gz
| | Sample_series_id | GSE29313
| | Sample_data_row_count | 54675
| | |
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