Search results for the GEO ID: GSE29375  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM726069 | GPL570 |
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HepG2 APRIL 0h
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HepG2 cells, untreated
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cell line: HepG2
cell type: hepatocyte derived cancer
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| Sample_geo_accession | GSM726069
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HepG2 cells were incubated with or without APRIL (200ng/ml) for 2, 6 and 12 hours after overnight serum starvation.
| | Sample_growth_protocol_ch1 | The human hepatocyte-derived cancer cell line HepG2 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA). RMA algorithm used with baseline set to the median of all.
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726069/suppl/GSM726069_HepG2_APRIL_0h.CEL.gz
| | Sample_series_id | GSE29375
| | Sample_data_row_count | 54675
| | |
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GSM726070 | GPL570 |
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HepG2 APRIL 2h
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HepG2 cells, treated for 2 hours with 200ng/ml APRIL
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cell line: HepG2
cell type: hepatocyte derived cancer
|
|
| Sample_geo_accession | GSM726070
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HepG2 cells were incubated with or without APRIL (200ng/ml) for 2, 6 and 12 hours after overnight serum starvation.
| | Sample_growth_protocol_ch1 | The human hepatocyte-derived cancer cell line HepG2 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA). RMA algorithm used with baseline set to the median of all.
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726070/suppl/GSM726070_HepG2_APRIL_2h.CEL.gz
| | Sample_series_id | GSE29375
| | Sample_data_row_count | 54675
| | |
|
GSM726071 | GPL570 |
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HepG2 APRIL 6h
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HepG2 cells, treated for 6 hours with 200ng/ml APRIL
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cell line: HepG2
cell type: hepatocyte derived cancer
|
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| Sample_geo_accession | GSM726071
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HepG2 cells were incubated with or without APRIL (200ng/ml) for 2, 6 and 12 hours after overnight serum starvation.
| | Sample_growth_protocol_ch1 | The human hepatocyte-derived cancer cell line HepG2 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA). RMA algorithm used with baseline set to the median of all.
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726071/suppl/GSM726071_HepG2_APRIL_6h.CEL.gz
| | Sample_series_id | GSE29375
| | Sample_data_row_count | 54675
| | |
|
GSM726072 | GPL570 |
|
HepG2 APRIL 12h
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HepG2 cells, treated for 12 hours with 200ng/ml APRIL
|
cell line: HepG2
cell type: hepatocyte derived cancer
|
|
| Sample_geo_accession | GSM726072
| | Sample_status | Public on May 20 2011
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | May 20 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HepG2 cells were incubated with or without APRIL (200ng/ml) for 2, 6 and 12 hours after overnight serum starvation.
| | Sample_growth_protocol_ch1 | The human hepatocyte-derived cancer cell line HepG2 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA). RMA algorithm used with baseline set to the median of all.
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726072/suppl/GSM726072_HepG2_APRIL_12h.CEL.gz
| | Sample_series_id | GSE29375
| | Sample_data_row_count | 54675
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