Search results for the GEO ID: GSE29384  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM726287 | GPL570 |
|
Tetracycline-inducible glioma cells dox 1
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Glioma cells expressing Cyr61
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cell line: LN229
expression: Cyr61
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gene expression was measured 24 hours + induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726287
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726287/suppl/GSM726287_AH_C16_+_1.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
|
GSM726288 | GPL570 |
|
Tetracycline-inducible glioma cells dox 2
|
Glioma cells expressing Cyr61
|
cell line: LN229
expression: Cyr61
|
gene expression was measured 24 hours + induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726288
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726288/suppl/GSM726288_AH_C16_+_2.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
|
GSM726289 | GPL570 |
|
Tetracycline-inducible glioma cells dox 3
|
Glioma cells expressing Cyr61
|
cell line: LN229
expression: Cyr61
|
gene expression was measured 24 hours + induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726289
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726289/suppl/GSM726289_AH_C16_+_3.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
|
GSM726290 | GPL570 |
|
Tetracycline-inducible glioma cells no dox 1
|
Glioma cells not expressing Cyr61
|
cell line: LN229
expression: control
|
gene expression was measured 24 hours - induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726290
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726290/suppl/GSM726290_AH_C16_-_1.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
|
GSM726291 | GPL570 |
|
Tetracycline-inducible glioma cells no dox 2
|
Glioma cells not expressing Cyr61
|
cell line: LN229
expression: control
|
gene expression was measured 24 hours - induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726291
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726291/suppl/GSM726291_AH_C16_-_2.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
|
GSM726292 | GPL570 |
|
Tetracycline-inducible glioma cells no dox 3
|
Glioma cells not expressing Cyr61
|
cell line: LN229
expression: control
|
gene expression was measured 24 hours - induction of Cyr61 protein in glioma cells
|
| Sample_geo_accession | GSM726292
| | Sample_status | Public on Apr 04 2012
| | Sample_submission_date | May 18 2011
| | Sample_last_update_date | Apr 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated ± doxycycline (1ug/ml) for 24 hours
| | Sample_growth_protocol_ch1 | Cy-1 cells were maintainted in Dulbecco’s modified minimal essential medium (DMEM) supplemented with 2% fetal bovine serum, 100U/ml penicillin, and 100ug/ml streptomycin in a 37C incubator with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using Qiashredders and Rneasy Mini Kit per manufacturer's protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 1ug RNA was used to generate cRNA according to the manufacturer’s protocol in the Affymetrix GeneChip® Expression Analysis Technical Manual
| | Sample_hyb_protocol | 15ug of labeled and fragmented cRNA was hybridized at 45C for 16 hours at 60rpm. GeneChips were then washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturer’s protocol
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G. Raw data files were generated and processed with Affymetrix AGCC software.
| | Sample_data_processing | The data were analyzed through Bioconductor package, RMA method was used as the background-correction, normalization, and probe summarization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Amy,,Haseley
| | Sample_contact_email | amy.haseley@osumc.edu
| | Sample_contact_department | Neurological Surgery
| | Sample_contact_institute | The Ohio State University
| | Sample_contact_address | 400 W. 12th Ave. 370CCC
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM726nnn/GSM726292/suppl/GSM726292_AH_C16_-_3.CEL.gz
| | Sample_series_id | GSE29384
| | Sample_data_row_count | 54675
| | |
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