Search results for the GEO ID: GSE29410  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM727409 | GPL570 |
|
Patient 1_Sub C
|
Patient 1_Sub C
|
gender: Female
tissue: sub cutaneous adipose tissue
|
JGG_1241_U133_plus2_160304
|
| Sample_geo_accession | GSM727409
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727409/suppl/GSM727409_JGG_1241_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
|
GSM727410 | GPL570 |
|
Patient 2_Sub C
|
Patient 2_Sub C
|
gender: Female
tissue: sub cutaneous adipose tissue
|
JGG_1242_U133_plus2_160304
|
| Sample_geo_accession | GSM727410
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727410/suppl/GSM727410_JGG_1242_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
|
GSM727411 | GPL570 |
|
Patient 3_Sub C
|
Patient 3_Sub C
|
gender: Female
tissue: sub cutaneous adipose tissue
|
JGG_1243_U133_plus2_160304
|
| Sample_geo_accession | GSM727411
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727411/suppl/GSM727411_JGG_1243_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
|
GSM727412 | GPL570 |
|
Patient 1_Om
|
Patient 1_Om
|
gender: Female
tissue: omental adipose tissue
|
JGG_1244_U133_plus2_160304
|
| Sample_geo_accession | GSM727412
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727412/suppl/GSM727412_JGG_1244_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
|
GSM727413 | GPL570 |
|
Patient 2_Om
|
Patient 2_Om
|
gender: Female
tissue: omental adipose tissue
|
JGG_1245_U133_plus2_160304
|
| Sample_geo_accession | GSM727413
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727413/suppl/GSM727413_JGG_1245_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
|
GSM727414 | GPL570 |
|
Patient 3_Om
|
Patient 3_Om
|
gender: Female
tissue: omental adipose tissue
|
JGG_1246_U133_plus2_160304
|
| Sample_geo_accession | GSM727414
| | Sample_status | Public on Jan 01 2012
| | Sample_submission_date | May 20 2011
| | Sample_last_update_date | Jan 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the adipose tissue tissue using an RNeasy Lipid tissue mini kit followed by incubation with RNase free DNase (Qiagen, Crawley, West Sussex) for DNA-free RNA following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 μg of high quality total RNA, verified using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies), was used with the Affymetrix Eukaryotic One-Cylce Target Labeling and Control reagents (#900493) following the manufacturers instructions to generate Biotin-labeled antisense cRNA.
| | Sample_hyb_protocol | The labeled and fragmented cRNA (10mg) was used for the hybridization to the Affymetrix (Santa Clara, CA) gene chips U133 plus 2 for 16 hr at 45C. An automated process of washing and staining in the Affymetrix Fluidics Station 400 was performed.
| | Sample_scan_protocol | Affymetrix arrays were scanned using the Affymetrix 3000G scanner. Absolute values of expression were calculated from the scanned array using the Affymetrix Command Console v1 software.
| | Sample_data_processing | CEL files were transferred to MadMax website (https://madmax.bioinformatics.nl), and data analysis was performed using the statistical programming language R and the R libraries offered by the Bioconductor project. The data were first explored for quality control purposes and were normalized the GCRMA method within Bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claus,,Mayer
| | Sample_contact_email | claus@bioss.ac.uk
| | Sample_contact_institute | Biomathematics & Statistics Scotland
| | Sample_contact_address | Greenburn Road
| | Sample_contact_city | Aberdeen
| | Sample_contact_zip/postal_code | AB21 9SB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM727nnn/GSM727414/suppl/GSM727414_JGG_1246_U133_plus2_160304.CEL.gz
| | Sample_series_id | GSE29410
| | Sample_series_id | GSE29411
| | Sample_data_row_count | 54675
| | |
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