Result

Search results for the GEO ID: GSE29435

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM728705

GPL570

IKPRAB36, Control 1

IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: none treament duration: 48 hours

Gene expression data from IKPRAB36 cell line (Control).

Sample_geo_accession	GSM728705
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728705/suppl/GSM728705.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

GSM728706

GPL570

IKPRAB36, Control 2

IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: none treament duration: 48 hours

Gene expression data from IKPRAB36 cell line (Control).

Sample_geo_accession	GSM728706
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728706/suppl/GSM728706.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

GSM728707

GPL570

IKPRAB36, Control 3

IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: none treament duration: 48 hours

Gene expression data from IKPRAB36 cell line (Control).

Sample_geo_accession	GSM728707
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728707/suppl/GSM728707.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

GSM728708

GPL570

IKPRAB36, MPA 1

IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: 1 nM medroxyprogesterone acetate (MPA) treament duration: 48 hours

Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.

Sample_geo_accession	GSM728708
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728708/suppl/GSM728708.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

GSM728709

GPL570

IKPRAB36, MPA 2

IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: 1 nM medroxyprogesterone acetate (MPA) treament duration: 48 hours

Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.

Sample_geo_accession	GSM728709
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728709/suppl/GSM728709.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

GSM728710

GPL570

IKPRAB36, MPA 3

IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA

cell line: IKPRAB36 cell type: Ishikawa endometrial cancer cells expressing PRA and PRB treament: 1 nM medroxyprogesterone acetate (MPA) treament duration: 48 hours

Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.

Sample_geo_accession	GSM728710
Sample_status	Public on Jan 26 2012
Sample_submission_date	May 21 2011
Sample_last_update_date	Jan 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n	3).
Sample_growth_protocol_ch1	For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Standard Affymetrix protocol.
Sample_hyb_protocol	Standard Affymetrix protocol.
Sample_scan_protocol	Standard Affymetrix protocol.
Sample_data_processing	Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
Sample_platform_id	GPL570
Sample_contact_name	Paul,H,van der Horst
Sample_contact_email	p.vanderhorst@erasmusmc.nl
Sample_contact_laboratory	Gynaecologic Oncology Laboratory
Sample_contact_department	Obstetrics & Gynaecology
Sample_contact_institute	Erasmus University Medical Center
Sample_contact_address	PO box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000 CA
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728710/suppl/GSM728710.CEL.gz
Sample_series_id	GSE29435
Sample_series_id	GSE29437
Sample_data_row_count	54675

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