Search results for the GEO ID: GSE29437  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM728705 | GPL570 |
|
IKPRAB36, Control 1
|
IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: none
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line (Control).
|
| Sample_geo_accession | GSM728705
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728705/suppl/GSM728705.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728706 | GPL570 |
|
IKPRAB36, Control 2
|
IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: none
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line (Control).
|
| Sample_geo_accession | GSM728706
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728706/suppl/GSM728706.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728707 | GPL570 |
|
IKPRAB36, Control 3
|
IKPRAB36 cell line cultured for 48 hours in absence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: none
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line (Control).
|
| Sample_geo_accession | GSM728707
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728707/suppl/GSM728707.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728708 | GPL570 |
|
IKPRAB36, MPA 1
|
IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: 1 nM medroxyprogesterone acetate (MPA)
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.
|
| Sample_geo_accession | GSM728708
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728708/suppl/GSM728708.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728709 | GPL570 |
|
IKPRAB36, MPA 2
|
IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: 1 nM medroxyprogesterone acetate (MPA)
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.
|
| Sample_geo_accession | GSM728709
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728709/suppl/GSM728709.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728710 | GPL570 |
|
IKPRAB36, MPA 3
|
IKPRAB36 cell line cultured for 48 hours in presence of 1nm MPA
|
cell line: IKPRAB36
cell type: Ishikawa endometrial cancer cells expressing PRA and PRB
treament: 1 nM medroxyprogesterone acetate (MPA)
treament duration: 48 hours
|
Gene expression data from IKPRAB36 cell line treated with 1nM MPA for 48 hours.
|
| Sample_geo_accession | GSM728710
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = The IKPRAB36 cell line was cultured for 48h in the absence or presence of 1nM MPA (n | 3).
| | Sample_growth_protocol_ch1 | For all assays, cells were cultured in DMEM/F12 Glutamax culture medium supplemented with penicillin and streptomycin (Invitrogen), containing 5% charcoal stripped FCS (Invitrogen) with addition of hygromycin and neomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were placed on ice and lysed in Trizol (Invitrogen). Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728710/suppl/GSM728710.CEL.gz
| | Sample_series_id | GSE29435
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728711 | GPL570 |
|
Endometrial Cancer Specimen, Non-progressive 1
|
Snap-frozen endometrial cancer specimen from non-progressive patient
|
tissue: endometrial cancer
progressive status: non-progressive (no recurrence or metastasis)
|
Gene expression data from non-progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728711
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728711/suppl/GSM728711.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728712 | GPL570 |
|
Endometrial Cancer Specimen, Non-progressive 2
|
Snap-frozen endometrial cancer specimen from non-progressive patient
|
tissue: endometrial cancer
progressive status: non-progressive (no recurrence or metastasis)
|
Gene expression data from non-progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728712
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728712/suppl/GSM728712.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728713 | GPL570 |
|
Endometrial Cancer Specimen, Non-progressive 3
|
Snap-frozen endometrial cancer specimen from non-progressive patient
|
tissue: endometrial cancer
progressive status: non-progressive (no recurrence or metastasis)
|
Gene expression data from non-progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728713
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728713/suppl/GSM728713.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728714 | GPL570 |
|
Endometrial Cancer Specimen, Non-progressive 4
|
Snap-frozen endometrial cancer specimen from non-progressive patient
|
tissue: endometrial cancer
progressive status: non-progressive (no recurrence or metastasis)
|
Gene expression data from non-progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728714
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728714/suppl/GSM728714.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728715 | GPL570 |
|
Endometrial Cancer Specimen, Progressive 1
|
Snap-frozen endometrial cancer specimen from progressive patient
|
tissue: endometrial cancer
progressive status: progressive (recurrence or metastasis)
|
Gene expression data from progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728715
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728715/suppl/GSM728715.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728716 | GPL570 |
|
Endometrial Cancer Specimen, Progressive 2
|
Snap-frozen endometrial cancer specimen from progressive patient
|
tissue: endometrial cancer
progressive status: progressive (recurrence or metastasis)
|
Gene expression data from progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728716
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728716/suppl/GSM728716.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728717 | GPL570 |
|
Endometrial Cancer Specimen, Progressive 3
|
Snap-frozen endometrial cancer specimen from progressive patient
|
tissue: endometrial cancer
progressive status: progressive (recurrence or metastasis)
|
Gene expression data from progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728717
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728717/suppl/GSM728717.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
|
GSM728718 | GPL570 |
|
Endometrial Cancer Specimen, Progressive 4
|
Snap-frozen endometrial cancer specimen from progressive patient
|
tissue: endometrial cancer
progressive status: progressive (recurrence or metastasis)
|
Gene expression data from progressive endometrial cancer specimen.
|
| Sample_geo_accession | GSM728718
| | Sample_status | Public on Jan 26 2012
| | Sample_submission_date | May 21 2011
| | Sample_last_update_date | Jan 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Patient tissue samples were sectioned (5 μm, cryostat) and every 10th section was HE stained and revised by the pathologist (PCE) to assess tumor load. Only sections containing >80% tumor were lysed in Trizol (Invitrogen) and sonified for 1 min. Phase separation was accomplished with 0.2 ml chloroform and centrifugation for 15 min. The supernatant was transferred and isopropanol was added for RNA precipitation. The precipitated RNA was washed with 75% ethanol. All RNA was cleaned with the RNeasy MinElute cleanup kit (Qiagen, Venlo, The Netherlands). Amount and quality of the RNA was assessed by using the Nanodrop (Nanodrop, Wilmington, DE, USA) and Bio-analyzer (Aligent, Santa Clara, CA, USA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Using RMAexpress (Robust Multi-array Analysis), normalization of raw data was performed to be able to produce gene lists.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,H,van der Horst
| | Sample_contact_email | p.vanderhorst@erasmusmc.nl
| | Sample_contact_laboratory | Gynaecologic Oncology Laboratory
| | Sample_contact_department | Obstetrics & Gynaecology
| | Sample_contact_institute | Erasmus University Medical Center
| | Sample_contact_address | PO box 2040
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3000 CA
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM728nnn/GSM728718/suppl/GSM728718.CEL.gz
| | Sample_series_id | GSE29436
| | Sample_series_id | GSE29437
| | Sample_data_row_count | 54675
| | |
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