Search results for the GEO ID: GSE29523  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM730677 | GPL570 |
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CD34+CD45RA+Lin- HPCs transduced with A2M
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CD34+CD45RA+Lin- HPCs
|
tissue: umbilical cord blood
genotype/variation: transduced with A2M
cell type: hematopoietic progenitors
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CD45RA+M
Gene expression data from cell transduced with nuclear-trapped AF1q/MLLT11 (A2M)
|
| Sample_geo_accession | GSM730677
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were sorted differentially by FACS, exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with SCF, FL and TPO, cells were harvested, and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condition in the presence of SCF, FL and TPO
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730677/suppl/GSM730677.CEL.gz
| | Sample_series_id | GSE29523
| | Sample_data_row_count | 54675
| | |
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GSM730678 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with empty vector
|
CD34+CD45RA+Lin- HPCs
|
tissue: umbilical cord blood
genotype/variation: transduced with empty vector
cell type: hematopoietic progenitors
|
CD45RA+P
Gene expression data from cell transduced with empty vector
|
| Sample_geo_accession | GSM730678
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were sorted differentially by FACS, exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with SCF, FL and TPO, cells were harvested, and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condition in the presence of SCF, FL and TPO
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730678/suppl/GSM730678.CEL.gz
| | Sample_series_id | GSE29523
| | Sample_data_row_count | 54675
| | |
|
GSM730679 | GPL570 |
|
CD34+CD45RA-Lin- HPCs transduced with A2M
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
genotype/variation: transduced with A2M
cell type: hematopoietic progenitors
|
CD45RA-M
Gene expression data from cell transduced with nuclear-trapped AF1q/MLLT11 (A2M)
|
| Sample_geo_accession | GSM730679
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were sorted differentially by FACS, exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with SCF, FL and TPO, cells were harvested, and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condition in the presence of SCF, FL and TPO
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730679/suppl/GSM730679.CEL.gz
| | Sample_series_id | GSE29523
| | Sample_data_row_count | 54675
| | |
|
GSM730680 | GPL570 |
|
CD34+CD45RA-Lin- HPCs transduced with empty vector
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
genotype/variation: transduced with empty vector
cell type: hematopoietic progenitors
|
CD45RA-P
Gene expression data from cell transduced with empty vector
|
| Sample_geo_accession | GSM730680
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were sorted differentially by FACS, exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with SCF, FL and TPO, cells were harvested, and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condition in the presence of SCF, FL and TPO
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730680/suppl/GSM730680.CEL.gz
| | Sample_series_id | GSE29523
| | Sample_data_row_count | 54675
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