Search results for the GEO ID: GSE29524  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM730681 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto BSA
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CD34+CD45RA-Lin- HPCs
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tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with empty vector
treatment group: control
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BCE_01
Gene expression data from control cells transduced with empty vector
|
| Sample_geo_accession | GSM730681
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730681/suppl/GSM730681.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
|
GSM730682 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with A2M cultured onto BSA
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with A2M
treatment group: control
|
BCE_02
Gene expression data from control cells transduced with A2M lentiviral vector
|
| Sample_geo_accession | GSM730682
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730682/suppl/GSM730682.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
|
GSM730683 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto 2 µg of Delta1ext-IgG
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with empty vector
treatment group: Notch-activated (2 µg)
|
BCE_03
Gene expression data from Notch-activated (2 µg) cells transduced with empty vector
|
| Sample_geo_accession | GSM730683
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730683/suppl/GSM730683.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
|
GSM730684 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with A2M cultured onto 2 µg of Delta1ext-IgG
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with A2M
treatment group: Notch-activated (2 µg)
|
BCE_04
Gene expression data from Notch-activated (2 µg) cells transduced with A2M lentiviral vector
|
| Sample_geo_accession | GSM730684
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730684/suppl/GSM730684.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
|
GSM730685 | GPL570 |
|
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto 5 µg of Delta1ext-IgG
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with empty vector
treatment group: Notch-activated (5 µg)
|
BCE_05
Gene expression data from Notch-activated (5 µg) cells transduced with empty vector
|
| Sample_geo_accession | GSM730685
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730685/suppl/GSM730685.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
|
GSM730686 | GPL570 |
|
CD34+CD45RA+Lin- HPCs overexpressing A2M cultured onto 5 µg of Delta1ext-IgG
|
CD34+CD45RA-Lin- HPCs
|
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with A2M
treatment group: Notch-activated (5 µg)
|
BCE_06
Gene expression data from Notch-activated (5 µg) cells transduced with A2M lentiviral vector
|
| Sample_geo_accession | GSM730686
| | Sample_status | Public on May 26 2011
| | Sample_submission_date | May 25 2011
| | Sample_last_update_date | May 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
| | Sample_growth_protocol_ch1 | Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA were extracted using the RNeasy Micro Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
| | Sample_hyb_protocol | cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner 2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marc,,Delord
| | Sample_contact_email | mdelord@gmail.com
| | Sample_contact_department | biostatistique/bioinformatique
| | Sample_contact_institute | Institut Universitaire d'Hématologie
| | Sample_contact_address | 1, avenue Claude Vellefaux
| | Sample_contact_city | Paris
| | Sample_contact_zip/postal_code | 75010
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM730nnn/GSM730686/suppl/GSM730686.CEL.gz
| | Sample_series_id | GSE29524
| | Sample_data_row_count | 54675
| | |
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