Result

Search results for the GEO ID: GSE29539

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM731299

GPL570

OECM1-mCherry

Head and neck cancer cell line OECM1 transfected with pmR-mCherry empty vector

cell line: OECM1 transfection: pmR-mCherry empty vector

Sample_geo_accession	GSM731299
Sample_status	Public on Feb 11 2013
Sample_submission_date	May 26 2011
Sample_last_update_date	Feb 11 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM731nnn/GSM731299/suppl/GSM731299_OECM1_mCherry.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM731nnn/GSM731299/suppl/GSM731299_OECM1_mCherry.CHP.gz
Sample_series_id	GSE29539
Sample_data_row_count	54675

GSM731300

GPL570

OECM1-let7i

Head and neck cancer cell line OECM1 transfected with pmCherry-let7i

cell line: OECM1 transfection: pmCherry-let7i

Sample_geo_accession	GSM731300
Sample_status	Public on Feb 11 2013
Sample_submission_date	May 26 2011
Sample_last_update_date	Feb 11 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	cultivated in RPMI + 10% fetal bovine serum
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
Sample_hyb_protocol	Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx
Sample_scan_protocol	Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
Sample_data_processing	We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each probe sets on the U133 2.0 oligonucleotide arrays.
Sample_platform_id	GPL570
Sample_contact_name	Muh-Hwa,,Yang
Sample_contact_email	mhyang2@vghtpe.gov.tw
Sample_contact_phone	+886-228235870
Sample_contact_fax	+886-228235870
Sample_contact_laboratory	Dr. M.H. Yang's Lab
Sample_contact_department	Institute of Clinical Medicine
Sample_contact_institute	National Yang-Ming University
Sample_contact_address	No.155, Sec.2, Li-Nong Street
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	112
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM731nnn/GSM731300/suppl/GSM731300_OECM1_let7i.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM731nnn/GSM731300/suppl/GSM731300_OECM1_let7i.CHP.gz
Sample_series_id	GSE29539
Sample_data_row_count	54675

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