Search results for the GEO ID: GSE29625 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM734252 | GPL570 |
|
Single blastomere derived hESC line W10-SBD1
|
W10-Single-Blastomere-Derived-hESC-line-R1
|
cell type: single blastomere derived hESC line
cell line: W10
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734252
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734252/suppl/GSM734252_W10_SBD1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734252/suppl/GSM734252_W10_SBD1.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734253 | GPL570 |
|
Single blastomere derived hESC line W10-SBD2
|
Single blastomere derived hESC line W10
|
cell type: single blastomere derived hESC line
cell line: W10
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734253
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734253/suppl/GSM734253_W10_SBD2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734253/suppl/GSM734253_W10_SBD2.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734255 | GPL570 |
|
Single blastomere derived hESC line SAB-113D-SBD6
|
Single blastomere derived hESC line SAB-113D
|
cell type: single blastomere derived hESC line
cell line: SAB-113D
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734255
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734255/suppl/GSM734255_SAB_113D_SBD6.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734255/suppl/GSM734255_SAB_113D_SBD6.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734256 | GPL570 |
|
ICM of whole embryo derived hESC line SG4-WED1
|
ICM of whole embryo derived hESC line SG4
|
cell type: ICM of whole embryo derived hESC line
cell line: SG4
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734256
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from ICM of whole embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734256/suppl/GSM734256_SG4_WED1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734256/suppl/GSM734256_SG4_WED1.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734257 | GPL570 |
|
ICM of whole embryo derived hESC line SG7-WED4
|
ICM of whole embryo derived hESC line SG7
|
cell type: ICM of whole embryo derived hESC line
cell line: SG7
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734257
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from ICM of whole embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734257/suppl/GSM734257_SG7_WED4.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734257/suppl/GSM734257_SG7_WED4.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734258 | GPL570 |
|
Clontech human universal reference total RNA ref-RNA-1
|
Clontech human universal reference total RNA
|
sample type: clontech human universal reference total rna
catalog #: 636538
|
Frozen ref-RNA samples from 2 different lot numbers were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734258
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 30 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Clontech, Mountain View, CA, USA.
| Sample_treatment_protocol_ch1 | Clontech human universal reference total RNA - Catalog No. 636538
| Sample_growth_protocol_ch1 | Clontech human universal reference total RNA - Catalog No. 636538
| Sample_growth_protocol_ch1 | These commercially available RNA samples were made by pooling total RNA extracts from a collection of different whole tissue sources, thus providing the broadest coverage of expressed genes.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | purchased from Clontech
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734258/suppl/GSM734258_ref_RNA_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734258/suppl/GSM734258_ref_RNA_1.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734259 | GPL570 |
|
Single blastomere derived hESC line SAB-113B-SBD4
|
Single blastomere derived hESC line SAB-113B
|
cell type: single blastomere derived hESC line
cell line: SAB-113B
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734259
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Protocol used by Expression Analysis (Expression Analysis, Inc., Durham, NC, USA).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734259/suppl/GSM734259_SAB_113B_SBD4.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734259/suppl/GSM734259_SAB_113B_SBD4.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734260 | GPL570 |
|
Single blastomere derived hESC line SAB-113B-SBD5
|
Single blastomere derived hESC line SAB-113B
|
cell type: single blastomere derived hESC line
cell line: SAB-113B
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734260
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734260/suppl/GSM734260_2_SAB_113B_SBD5.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734260/suppl/GSM734260_SAB_113B_SBD5.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734261 | GPL570 |
|
Single blastomere derived hESC line SAB-113D-SBD7
|
Single blastomere derived hESC line SAB-113D
|
cell type: single blastomere derived hESC line
cell line: SAB-113D
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734261
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734261/suppl/GSM734261_SAB_113D_SBD7.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734261/suppl/GSM734261_SAB_113D_SBD7.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
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GSM734262 | GPL570 |
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Single blastomere derived hESC line SAB-113D-SBD8
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Single blastomere derived hESC line SAB-113D
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cell type: single blastomere derived hESC line
cell line: SAB-113D
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734262
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from single blastomere of 8-cell stage embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734262/suppl/GSM734262_2_SAB_113D_SBD8.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734262/suppl/GSM734262_SAB_113D_SBD8.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
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GSM734263 | GPL570 |
|
ICM of whole embryo derived hESC line SG4-WED2
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ICM of whole embryo derived hESC line SG4
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cell type: ICM of whole embryo derived hESC line
cell line: SG4
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Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734263
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from ICM of whole embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734263/suppl/GSM734263_1_SG4_WED2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734263/suppl/GSM734263_2_SG4_WED2.CHP.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734264 | GPL570 |
|
ICM of whole embryo derived hESC line SG7-WED3
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ICM of whole embryo derived hESC line SG7
|
cell type: ICM of whole embryo derived hESC line
cell line: SG7
|
Frozen hESC pellets were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734264
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | StemLifeLine Inc.
| Sample_treatment_protocol_ch1 | hESC line derived from ICM of whole embryo
| Sample_growth_protocol_ch1 | Samples from 3 of the blastomere derived hESC lines (SAB-113B, SAB-113D and W10), and 2 of the whole embryo derived hESC lines (SG4 and SG7) established in our laboratory were collected for microarray analysis. All the selected hESC lines were adjusted to growth under feeder-free defined culture conditions on defined human matrix (CellStart, Invitrogen)-coated dishes in StemPro SFM serum-free hESC medium (Invitrogen) containing FGF (10 ng/ml), and passaged using accutase (Millipore, Temecula, CA, USA) for enzymatic cell separation. For each hESC line, cells were collected when the colonies showed 70-80% confluency in three biological replicates for SAB-113B and SAB-113D lines and 2 replicates for W10, SG4 and SG7 lines.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734264/suppl/GSM734264_1_SG7_WED3.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734264/suppl/GSM734264_SG7_WED3.CEL.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
|
GSM734265 | GPL570 |
|
Clontech human universal reference total RNA ref-RNA-2
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Clontech human universal reference total RNA
|
sample type: clontech human universal reference total rna
catalog #: 636538
|
Frozen ref-RNA samples from 2 different lot numbers were sent to Expression Analysis (Expression Analysis, Inc., Durham, NC, USA) for analysis.
|
Sample_geo_accession | GSM734265
| Sample_status | Public on Oct 21 2011
| Sample_submission_date | May 31 2011
| Sample_last_update_date | Oct 21 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Clontech, Mountain View, CA, USA.
| Sample_treatment_protocol_ch1 | Clontech human universal reference total RNA - Catalog No. 636538
| Sample_growth_protocol_ch1 | Clontech human universal reference total RNA - Catalog No. 636538
| Sample_growth_protocol_ch1 | These commercially available RNA samples were made by pooling total RNA extracts from a collection of different whole tissue sources, thus providing the broadest coverage of expressed genes.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | purchased from Clontech
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_hyb_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_scan_protocol | According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array).
| Sample_data_processing | Protocol used by Expression Analysis (Expression Analysis, Inc., Durham, NC, USA).
| Sample_platform_id | GPL570
| Sample_contact_name | Gnanaratnam,,Giritharan
| Sample_contact_email | thiyagiri1@yahoo.com
| Sample_contact_phone | 775-825-5688
| Sample_contact_institute | Nevada Center for Reproductive Medicine
| Sample_contact_address | 645 Sierra Rose Drive, Suite 205
| Sample_contact_city | Reno
| Sample_contact_state | Nevada
| Sample_contact_zip/postal_code | 89511
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734265/suppl/GSM734265_1_ref_RNA_2.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM734nnn/GSM734265/suppl/GSM734265_ref_RNA_2.CEL.gz
| Sample_series_id | GSE29625
| Sample_data_row_count | 54675
| |
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