Search results for the GEO ID: GSE29648  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM735040 | GPL1261 |
|
heart, male wt, rep1
|
left ventricle, male, wild-type
|
tissue: left ventricle
strain: C57BL/6
genotype: wild-type
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from wild-type male hearts
|
| Sample_geo_accession | GSM735040
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735040/suppl/GSM735040.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735041 | GPL1261 |
|
heart, male wt, rep2
|
left ventricle, male, wild-type
|
tissue: left ventricle
strain: C57BL/6
genotype: wild-type
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from wild-type male hearts
|
| Sample_geo_accession | GSM735041
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735041/suppl/GSM735041.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735042 | GPL1261 |
|
heart, male wt, rep3
|
left ventricle, male, wild-type
|
tissue: left ventricle
strain: C57BL/6
genotype: wild-type
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from wild-type male hearts
|
| Sample_geo_accession | GSM735042
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735042/suppl/GSM735042.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735043 | GPL1261 |
|
heart, male HCM, rep1
|
left ventricle, male, HCM
|
tissue: left ventricle
strain: C57BL/6
genotype: HCM
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from HCM male hearts
|
| Sample_geo_accession | GSM735043
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735043/suppl/GSM735043.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735044 | GPL1261 |
|
heart, male HCM, rep2
|
left ventricle, male, HCM
|
tissue: left ventricle
strain: C57BL/6
genotype: HCM
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from HCM male hearts
|
| Sample_geo_accession | GSM735044
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735044/suppl/GSM735044.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735045 | GPL1261 |
|
heart, male HCM, rep3
|
left ventricle, male, HCM
|
tissue: left ventricle
strain: C57BL/6
genotype: HCM
Sex: male
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from HCM male hearts
|
| Sample_geo_accession | GSM735045
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735045/suppl/GSM735045.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735046 | GPL1261 |
|
heart, female wt, rep1
|
left ventricle, female, wild-type
|
tissue: left ventricle
strain: C57BL/6
genotype: wild-type
Sex: female
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from wild-type female hearts
|
| Sample_geo_accession | GSM735046
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735046/suppl/GSM735046.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735047 | GPL1261 |
|
heart, female wt, rep2
|
left ventricle, female, wild-type
|
tissue: left ventricle
strain: C57BL/6
genotype: wild-type
Sex: female
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from wild-type female hearts
|
| Sample_geo_accession | GSM735047
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735047/suppl/GSM735047.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735048 | GPL1261 |
|
heart, female HCM, rep1
|
left ventricle, female, HCM
|
tissue: left ventricle
strain: C57BL/6
genotype: HCM
Sex: female
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from HCM female hearts
|
| Sample_geo_accession | GSM735048
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735048/suppl/GSM735048.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
GSM735049 | GPL1261 |
|
heart, female HCM, rep2
|
left ventricle, female, HCM
|
tissue: left ventricle
strain: C57BL/6
genotype: HCM
Sex: female
diet: phytoestrogen-supplemented casein-based
age: 2mo
|
Gene expression data from HCM female hearts
|
| Sample_geo_accession | GSM735049
| | Sample_status | Public on Jun 02 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 02 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | At 2 months old, mice were anesthetized with inhaled isoflurane, sacrificed by cervical dislocation, and hearts were rapidly excised. Hearts were exsanguinated in ice-cold, sterile PBS and left ventricles were flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | Mice were housed until 2 months of age under standard conditions and fed ad libitum a casein-based diet supplemented with the phytoestrogens genistein and daidzein.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRI reagent according to the manufacturer's instructions. RNA was further purified with a Qiagen RNeasy kit. For each sample, equal amounts of RNA from two or three animals was pooled.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were amplified and labeled according to the Affymetrix instructions using 6 μg total RNA.
| | Sample_hyb_protocol | cRNA were hybridized 16 hours at 45 degrees Celsius on the Affymetrix Mouse Genome 430 2.0 GeneChip. Chips were washed and stained in a Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Gene Chip Scanner 3000 6G.
| | Sample_data_processing | The data were analyzed with the Robust Multiarray Averaging (RMA) method in Affymetrix Expression Console using default settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Leslie,,Leinwand
| | Sample_contact_email | Leslie.Leinwand@colorado.edu
| | Sample_contact_department | MCD Biology
| | Sample_contact_institute | University of Colorado at Boulder
| | Sample_contact_address | UCB347
| | Sample_contact_city | Boulder
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80309
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735049/suppl/GSM735049.CEL.gz
| | Sample_series_id | GSE29648
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
