Search results for the GEO ID: GSE29652  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM735094 | GPL570 |
|
Braak I-II, APOE e4+ temporal cortex astrocytes
|
JS1
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735094
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735094/suppl/GSM735094.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735095 | GPL570 |
|
Braak I-II, APOE e4+ sample 2
|
JS2
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735095
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735095/suppl/GSM735095.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735096 | GPL570 |
|
Braak I-II, APOE e4+ sample 3
|
JS3
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735096
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735096/suppl/GSM735096.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735097 | GPL570 |
|
Braak I-II, APOE e4- temporal cortex astrocytes
|
JS4
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735097
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735097/suppl/GSM735097.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735098 | GPL570 |
|
Braak I-II, APOE e4- sample 2
|
JS5
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735098
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735098/suppl/GSM735098.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735099 | GPL570 |
|
Braak I-II, APOE e4- sample 3
|
JS6
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak I-II
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735099
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735099/suppl/GSM735099.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735100 | GPL570 |
|
Braak III-IV, APOE e4+ temporal cortex astrocytes
|
JS7
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735100
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735100/suppl/GSM735100.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735101 | GPL570 |
|
Braak III-IV, APOE e4+ sample 2
|
JS8
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735101
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735101/suppl/GSM735101.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735102 | GPL570 |
|
Braak III-IV, APOE e4+ sample 3
|
JS9
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735102
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735102/suppl/GSM735102.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735103 | GPL570 |
|
Braak III-IV, APOE e4- temporal cortex astrocytes
|
JS10
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735103
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735103/suppl/GSM735103.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735104 | GPL570 |
|
Braak III-IV, APOE e4- sample 2
|
JS11
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735104
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735104/suppl/GSM735104.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735105 | GPL570 |
|
Braak III-IV, APOE e4- sample 3
|
JS12
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak III-IV
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735105
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735105/suppl/GSM735105.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735106 | GPL570 |
|
Braak V-VI, APOE e4+ temporal cortex astrocytes
|
JS13
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735106
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735106/suppl/GSM735106.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735107 | GPL570 |
|
Braak V-VI, APOE e4+ sample 2
|
JS14
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735107
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735107/suppl/GSM735107.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735108 | GPL570 |
|
Braak V-VI, APOE e4+ sample 3
|
JS15
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4+
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735108
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735108/suppl/GSM735108.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735109 | GPL570 |
|
Braak V-VI, APOE e4- sample 1
|
JS16
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735109
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735109/suppl/GSM735109.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735110 | GPL570 |
|
Braak V-VI, APOE e4- sample 2
|
JS17
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735110
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735110/suppl/GSM735110.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
GSM735111 | GPL570 |
|
Braak V-VI, APOE e4- sample 3
|
JS18
|
tissue: temporal cortex
cell type: astrocytes
braak stage: Braak V-VI
apoe status: APOE e4-
|
Gene expression data from human astrocytes
|
| Sample_geo_accession | GSM735111
| | Sample_status | Public on Jun 01 2011
| | Sample_submission_date | Jun 01 2011
| | Sample_last_update_date | Jun 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 7um frozen temporal cortex sections were fixed in acetone and immunostained for GFAP using a rapid staining protocol. They were then washed and dehydrated through graded ethanol concentrations (70%, 95%, 100%) and cleared in xylene.
| | Sample_treatment_protocol_ch1 | From each individual case, approximately 1500 astrocytes were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 7µg of cRNA molecules were heat fragmented and applied to the Human Genome HGU133 Plus 2.0 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were using Array Assist 3 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM735nnn/GSM735111/suppl/GSM735111.CEL.gz
| | Sample_series_id | GSE29652
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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