Search results for the GEO ID: GSE29743  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM737424 | GPL339 |
|
control B16-F10 cells, biological rep1
|
control B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: none
|
Gene expression data from control B16-F10 cells.
|
| Sample_geo_accession | GSM737424
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737424/suppl/GSM737424.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737425 | GPL339 |
|
control B16-F10 cells, biological rep2
|
control B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: none
|
Gene expression data from control B16-F10 cells.
|
| Sample_geo_accession | GSM737425
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737425/suppl/GSM737425.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737426 | GPL339 |
|
control B16-F10 cells, biological rep3
|
control B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: none
|
Gene expression data from control B16-F10 cells.
|
| Sample_geo_accession | GSM737426
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737426/suppl/GSM737426.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737427 | GPL339 |
|
control B16-F10 cells, biological rep4
|
control B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: none
|
Gene expression data from control B16-F10 cells.
|
| Sample_geo_accession | GSM737427
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737427/suppl/GSM737427.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737428 | GPL339 |
|
control B16-F10 cells, biological rep5
|
control B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: none
|
Gene expression data from control B16-F10 cells.
|
| Sample_geo_accession | GSM737428
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737428/suppl/GSM737428.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737429 | GPL339 |
|
heat-shocked B16-F10 cells, biological rep1
|
heat-shocked B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: heat shock
|
Gene expression data from B16-F10 cells treated with heat shock.
|
| Sample_geo_accession | GSM737429
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737429/suppl/GSM737429.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737430 | GPL339 |
|
heat-shocked B16-F10 cells, biological rep2
|
heat-shocked B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: heat shock
|
Gene expression data from B16-F10 cells treated with heat shock.
|
| Sample_geo_accession | GSM737430
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737430/suppl/GSM737430.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737431 | GPL339 |
|
heat-shocked B16-F10 cells, biological rep3
|
heat-shocked B16-F10 cells
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: heat shock
|
Gene expression data from B16-F10 cells treated with heat shock.
|
| Sample_geo_accession | GSM737431
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737431/suppl/GSM737431.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737432 | GPL339 |
|
B16-F10 cells treated with Lipofectamine, biological rep1
|
B16-F10 cells treated with Lipofectamine
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: Lipofectamine
|
Gene expression data from B16-F10 cells treated with Lipofectamine.
|
| Sample_geo_accession | GSM737432
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737432/suppl/GSM737432.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737433 | GPL339 |
|
B16-F10 cells treated with Lipofectamine, biological rep2
|
B16-F10 cells treated with Lipofectamine
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: Lipofectamine
|
Gene expression data from B16-F10 cells treated with Lipofectamine.
|
| Sample_geo_accession | GSM737433
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737433/suppl/GSM737433.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737434 | GPL339 |
|
B16-F10 cells treated with Lipofectamine, biological rep3
|
B16-F10 cells treated with Lipofectamine
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: Lipofectamine
|
Gene expression data from B16-F10 cells treated with Lipofectamine.
|
| Sample_geo_accession | GSM737434
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737434/suppl/GSM737434.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737435 | GPL339 |
|
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep1
|
B16-F10 cells treated with benzyl alcohol
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: benzyl alcohol
|
Gene expression data from B16-F10 cells treated with benzyl alcohol.
|
| Sample_geo_accession | GSM737435
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737435/suppl/GSM737435.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737436 | GPL339 |
|
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep2
|
B16-F10 cells treated with benzyl alcohol
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: benzyl alcohol
|
Gene expression data from B16-F10 cells treated with benzyl alcohol.
|
| Sample_geo_accession | GSM737436
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737436/suppl/GSM737436.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
GSM737437 | GPL339 |
|
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep3
|
B16-F10 cells treated with benzyl alcohol
|
cell line: B16-F10
strain: C57BL/6J
cell type: melanoma cells
treatment: benzyl alcohol
|
Gene expression data from B16-F10 cells treated with benzyl alcohol.
|
| Sample_geo_accession | GSM737437
| | Sample_status | Public on Jun 04 2011
| | Sample_submission_date | Jun 03 2011
| | Sample_last_update_date | Jun 04 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Heat shock was performed for 1 h at 42.5°C with 30 min recovery at 37°C. For microarray analysis of LA effect, cells were treated with Lipofectamine 2000 (Invitrogen) for 3 h in Opti-MEM (Gibco BRL) medium, the liposomes-containing medium was replaced with RPMI supplemented with 10% FBS, and the cells were allowed to recover for 30 min at 37°C. For microarray analysis of BA effect, cells were treated with 40 mM benzyl alcohol for 1h at 37°C, and then allowed to recover in fresh medium for 3h at 37°C.
| | Sample_growth_protocol_ch1 | The mouse melanoma B16-F10 cells (ATCC CRL-6475T) were grown in a CO2 incubator at 37°C in RPMI medium (Sigma) supplemented with 10% fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (8 ug) was used for synthesis of double-stranded cDNA according to the manufacturer's instructions. Half of the cDNA volume was used for synthesis of biotinylated cRNA with the BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of cRNA were hybridized for 16 hr at 45C on Mouse Expression Arrays 430A (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | Data was acquired using GCOS 1.2 software (Affymetrix). The preprocessing was performed by Robust Multiarray Analysis (RMA, Bioconductor).
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michal,,Swierniak
| | Sample_contact_department | Nuclear Medicine and Endocrine Oncology
| | Sample_contact_institute | MSC Memorial Cancer Center and Institute of Oncology
| | Sample_contact_address | Wybrzeze Armii Krajowej 15
| | Sample_contact_city | Gliwice
| | Sample_contact_zip/postal_code | 44-101
| | Sample_contact_country | Poland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM737nnn/GSM737437/suppl/GSM737437.CEL.gz
| | Sample_series_id | GSE29743
| | Sample_data_row_count | 22690
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
