Search results for the GEO ID: GSE29828 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM739148 | GPL570 |
|
3 uM EPZ004777 treated, DAY 2, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 2
biological rep: 1
|
Gene expression data from 3 uM EPZ004777 treated, DAY 2, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739148
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739148/suppl/GSM739148.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739149 | GPL570 |
|
3 uM EPZ004777 treated, DAY 2, biological rep.2, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 2
biological rep: 2
|
Gene expression data from 3 uM EPZ004777 treated, DAY 2, biological rep.2, MV4-11 cells
|
Sample_geo_accession | GSM739149
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739149/suppl/GSM739149.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739150 | GPL570 |
|
3 uM EPZ004777 treated, DAY 2, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 2
biological rep: 3
|
Gene expression data from 3 uM EPZ004777 treated, DAY 2, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739150
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739150/suppl/GSM739150.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739151 | GPL570 |
|
Vehicle treated, DAY 2, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 2
biological rep: 1
|
Gene expression data from Vehicle treated, DAY 2, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739151
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739151/suppl/GSM739151.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739152 | GPL570 |
|
Vehicle treated, DAY 2, biological rep.2, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 2
biological rep: 2
|
Gene expression data from Vehicle treated, DAY 2, biological rep.2, MV4-11 cells
|
Sample_geo_accession | GSM739152
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739152/suppl/GSM739152.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739153 | GPL570 |
|
Vehicle treated, DAY 2, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 2
biological rep: 3
|
Gene expression data from Vehicle treated, DAY 2, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739153
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739153/suppl/GSM739153.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739154 | GPL570 |
|
3 uM EPZ004777 treated, DAY 4, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 4
biological rep: 1
|
Gene expression data from 3 uM EPZ004777 treated, DAY 4, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739154
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739154/suppl/GSM739154.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739155 | GPL570 |
|
3 uM EPZ004777 treated, DAY 4, biological rep.2, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 4
biological rep: 2
|
Gene expression data from 3 uM EPZ004777 treated, DAY 4, biological rep.2, MV4-11 cells
|
Sample_geo_accession | GSM739155
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739155/suppl/GSM739155.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739156 | GPL570 |
|
3 uM EPZ004777 treated, DAY 4, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 4
biological rep: 3
|
Gene expression data from 3 uM EPZ004777 treated, DAY 4, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739156
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739156/suppl/GSM739156.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739157 | GPL570 |
|
Vehicle treated, DAY 4, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 4
biological rep: 1
|
Gene expression data from Vehicle treated, DAY 4, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739157
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739157/suppl/GSM739157.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739159 | GPL570 |
|
Vehicle treated, DAY 4, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 4
biological rep: 3
|
Gene expression data from Vehicle treated, DAY 4, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739159
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739159/suppl/GSM739159.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739160 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 1
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739160
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739160/suppl/GSM739160.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739161 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.2, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 2
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.2, MV4-11 cells
|
Sample_geo_accession | GSM739161
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739161/suppl/GSM739161.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739162 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 3
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739162
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739162/suppl/GSM739162.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739163 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.1, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 6
biological rep: 1
|
Gene expression data from Vehicle treated, DAY 6, biological rep.1, MV4-11 cells
|
Sample_geo_accession | GSM739163
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739163/suppl/GSM739163.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739164 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.2, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 6
biological rep: 2
|
Gene expression data from Vehicle treated, DAY 6, biological rep.2, MV4-11 cells
|
Sample_geo_accession | GSM739164
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739164/suppl/GSM739164.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739165 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.3, MV4-11 cells
|
MV4-11 cells
|
cell line: MV4-11
cell line description: biphenotypic B myelomonocytic leukemia (MLL-AF4 rearragenment)
growth conditions: IMDM with 10% FBS
agent: Vehicle
time: day 6
biological rep: 3
|
Gene expression data from Vehicle treated, DAY 6, biological rep.3, MV4-11 cells
|
Sample_geo_accession | GSM739165
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739165/suppl/GSM739165.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739166 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.1, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 1
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.1, MOLM-13 cells
|
Sample_geo_accession | GSM739166
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739166/suppl/GSM739166.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739167 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.2, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 2
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.2, MOLM-13 cells
|
Sample_geo_accession | GSM739167
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739167/suppl/GSM739167.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739168 | GPL570 |
|
3 uM EPZ004777 treated, DAY 6, biological rep.3, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: EPZ004777
time: day 6
biological rep: 3
|
Gene expression data from 3 uM EPZ004777 treated, DAY 6, biological rep.3, MOLM-13 cells
|
Sample_geo_accession | GSM739168
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739168/suppl/GSM739168.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739169 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.1, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: Vehicle
time: day 6
biological rep: 1
|
Gene expression data from Vehicle treated, DAY 6, biological rep.1, MOLM-13 cells
|
Sample_geo_accession | GSM739169
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739169/suppl/GSM739169.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739170 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.2, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: Vehicle
time: day 6
biological rep: 2
|
Gene expression data from Vehicle treated, DAY 6, biological rep.2, MOLM-13 cells
|
Sample_geo_accession | GSM739170
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739170/suppl/GSM739170.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
GSM739171 | GPL570 |
|
Vehicle treated, DAY 6, biological rep.3, MOLM-13 cells
|
MOLM-13 cells
|
cell line: MOLM-13
cell line description: acute myeloid leukemia (MLL-AF9 rearrangement)
growth conditions: RPMI with 10% FBS
agent: Vehicle
time: day 6
biological rep: 3
|
Gene expression data from Vehicle treated, DAY 6, biological rep.3, MOLM-13 cells
|
Sample_geo_accession | GSM739171
| Sample_status | Public on Jul 11 2011
| Sample_submission_date | Jun 08 2011
| Sample_last_update_date | Jul 11 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolation was peformed by Expression Analysis, Inc. (Durham, NC). RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol, including the on-column DNase I digestion. RNA integrity and quantity was measured using an Agilent Bioanalyzer 2100.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA labeling was peformed by Expression Analysis, Inc. (Durham, NC). Total RNA was converted cDNA and in vitro transcribed to biotin-labeled anti-sense RNA, using the Enzo Single Round RNA Amplification and Biotin Labeling System (Enzo # ENZ-42420-10) according to the manufacturer’s protocol.
| Sample_hyb_protocol | RNA hybridization to Affymetrix HG-U133 Plus 2 arrays was performed by Expression Analysis, Inc. (Durham, NC). Standard Affymetrix protocols were used with the Affymetrix FS450 Fluidics Station and the Affymetrix Hybridization, Washing and Staining kits.
| Sample_scan_protocol | Microarray scanning and data extraction was perfomed by Expression Analysis, Inc. (Durham, NC). The Affymetrix GeneChip Scanner 3000 7G was used. GeneChip Command Console (Affymetrix) was used to drive the scanners. Expression Console (Affymetrix) was used to extract data from the scanned images.
| Sample_data_processing | Expression file matrix data was generated using Genepattern Software for the Broad Institute (http://genepattern.broadinstitute.org/gp) with the ExpressionFileCreator Module. GCRMA method was used with quantile normalization.
| Sample_platform_id | GPL570
| Sample_contact_name | Jesse,Jerome,Smith
| Sample_contact_email | jsmith@epizyme.com
| Sample_contact_phone | 617-500-0596
| Sample_contact_department | Biological Sciences
| Sample_contact_institute | Epizyme
| Sample_contact_address | 325 Vassar Street, Suite 2B
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_contact_web_link | http://www.epizyme.com
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM739nnn/GSM739171/suppl/GSM739171.CEL.gz
| Sample_series_id | GSE29828
| Sample_data_row_count | 54675
| |
|
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