Search results for the GEO ID: GSE29881  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM740042 | GPL570 |
|
Control, HUAEC, rep 1
|
HUAEC, control
|
cell type: endothelial cell
treatment: ethanol control
treatment duration: 24 hrs
|
Gene expression data from control HUAECs.
|
| Sample_geo_accession | GSM740042
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740042/suppl/GSM740042.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740043 | GPL570 |
|
Control, HUAEC, rep 2
|
HUAEC, control
|
cell type: endothelial cell
treatment: ethanol control
treatment duration: 24 hrs
|
Gene expression data from control HUAECs.
|
| Sample_geo_accession | GSM740043
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740043/suppl/GSM740043.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740044 | GPL570 |
|
Control, HUAEC, rep 3
|
HUAEC, control
|
cell type: endothelial cell
treatment: ethanol control
treatment duration: 24 hrs
|
Gene expression data from control HUAECs.
|
| Sample_geo_accession | GSM740044
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740044/suppl/GSM740044.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740045 | GPL570 |
|
Control, HUAEC, rep 4
|
HUAEC, control
|
cell type: endothelial cell
treatment: ethanol control
treatment duration: 24 hrs
|
Gene expression data from control HUAECs.
|
| Sample_geo_accession | GSM740045
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740045/suppl/GSM740045.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740046 | GPL570 |
|
Control, HUAEC, rep 5
|
HUAEC, control
|
cell type: endothelial cell
treatment: ethanol control
treatment duration: 24 hrs
|
Gene expression data from control HUAECs.
|
| Sample_geo_accession | GSM740046
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740046/suppl/GSM740046.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740047 | GPL570 |
|
Estradiol, HUAEC, rep 1
|
HUAEC treated with 1 nM estradiol for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol
treatment duration: 24 hrs
|
Gene expression data from estradiol-treated HUAECs.
|
| Sample_geo_accession | GSM740047
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740047/suppl/GSM740047.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740048 | GPL570 |
|
Estradiol, HUAEC, rep 2
|
HUAEC treated with 1 nM estradiol for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol
treatment duration: 24 hrs
|
Gene expression data from estradiol-treated HUAECs.
|
| Sample_geo_accession | GSM740048
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740048/suppl/GSM740048.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740049 | GPL570 |
|
Estradiol, HUAEC, rep 3
|
HUAEC treated with 1 nM estradiol for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol
treatment duration: 24 hrs
|
Gene expression data from estradiol-treated HUAECs.
|
| Sample_geo_accession | GSM740049
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740049/suppl/GSM740049.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740050 | GPL570 |
|
Estradiol, HUAEC, rep 4
|
HUAEC treated with 1 nM estradiol for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol
treatment duration: 24 hrs
|
Gene expression data from estradiol-treated HUAECs.
|
| Sample_geo_accession | GSM740050
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740050/suppl/GSM740050.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740051 | GPL570 |
|
Estradiol, HUAEC, rep 5
|
HUAEC treated with 1 nM estradiol for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol
treatment duration: 24 hrs
|
Gene expression data from estradiol-treated HUAECs.
|
| Sample_geo_accession | GSM740051
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740051/suppl/GSM740051.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740052 | GPL570 |
|
oxLDL, HUAEC, rep 1
|
HUAEC treated with 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: oxLDL
treatment duration: 24 hrs
|
Gene expression data from oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740052
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740052/suppl/GSM740052.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740053 | GPL570 |
|
oxLDL, HUAEC, rep 2
|
HUAEC treated with 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: oxLDL
treatment duration: 24 hrs
|
Gene expression data from oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740053
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740053/suppl/GSM740053.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740054 | GPL570 |
|
oxLDL, HUAEC, rep 3
|
HUAEC treated with 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: oxLDL
treatment duration: 24 hrs
|
Gene expression data from oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740054
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740054/suppl/GSM740054.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740055 | GPL570 |
|
oxLDL, HUAEC, rep 4
|
HUAEC treated with 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: oxLDL
treatment duration: 24 hrs
|
Gene expression data from oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740055
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740055/suppl/GSM740055.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740056 | GPL570 |
|
oxLDL, HUAEC, rep 5
|
HUAEC treated with 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: oxLDL
treatment duration: 24 hrs
|
Gene expression data from oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740056
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740056/suppl/GSM740056.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740057 | GPL570 |
|
Estradiol + oxLDL, HUAEC, rep 1
|
HUAEC treated with 1 nM estradiol and 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol and oxLDL
treatment duration: 24 hrs
|
Gene expression data from estradiol- and oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740057
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740057/suppl/GSM740057.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740058 | GPL570 |
|
Estradiol + oxLDL, HUAEC, rep 2
|
HUAEC treated with 1 nM estradiol and 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol and oxLDL
treatment duration: 24 hrs
|
Gene expression data from estradiol- and oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740058
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740058/suppl/GSM740058.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740059 | GPL570 |
|
Estradiol + oxLDL, HUAEC, rep 3
|
HUAEC treated with 1 nM estradiol and 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol and oxLDL
treatment duration: 24 hrs
|
Gene expression data from estradiol- and oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740059
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740059/suppl/GSM740059.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740060 | GPL570 |
|
Estradiol + oxLDL, HUAEC, rep 4
|
HUAEC treated with 1 nM estradiol and 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol and oxLDL
treatment duration: 24 hrs
|
Gene expression data from estradiol- and oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740060
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740060/suppl/GSM740060.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
GSM740061 | GPL570 |
|
Estradiol + oxLDL, HUAEC, rep 5
|
HUAEC treated with 1 nM estradiol and 100 µg/ml oxLDL for 24 h
|
cell type: endothelial cell
treatment: 17β-estradiol and oxLDL
treatment duration: 24 hrs
|
Gene expression data from estradiol- and oxLDL-treated HUAECs.
|
| Sample_geo_accession | GSM740061
| | Sample_status | Public on Jun 10 2011
| | Sample_submission_date | Jun 09 2011
| | Sample_last_update_date | Jun 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol, 100 µg/ml oxLDL, estradiol + oxLDL, or its vehicle (0.1% ethanol) for 24 hours. Medium was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUAECs were isolated and grown in human endothelial cell-specific medium EBM-2 supplemented with EGM-2 (Lonza) in an incubator at 37 ºC with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM740nnn/GSM740061/suppl/GSM740061.CEL.gz
| | Sample_series_id | GSE29881
| | Sample_data_row_count | 54613
| | |
|
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