Search results for the GEO ID: GSE29975  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM741985 | GPL1261 |
|
FOG1+/- Meg, biological rep1
|
FOG1+/- Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1+/-
tissue: fetal liver
cell type: megakaryocyte
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Meg_3850
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| Sample_geo_accession | GSM741985
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741985/suppl/GSM741985.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
|
GSM741986 | GPL1261 |
|
FOG1-/- ki/ki Meg, biological rep1
|
FOG1-/- ki/ki Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1-/- ki/ki
tissue: fetal liver
cell type: megakaryocyte
|
Meg_3851
|
| Sample_geo_accession | GSM741986
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741986/suppl/GSM741986.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
|
GSM741987 | GPL1261 |
|
FOG1+/- Meg, biological rep2
|
FOG1+/- Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1+/-
tissue: fetal liver
cell type: megakaryocyte
|
Meg_4398
|
| Sample_geo_accession | GSM741987
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741987/suppl/GSM741987.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
|
GSM741988 | GPL1261 |
|
FOG1-/- ki/ki Meg, biological rep2
|
FOG1-/- ki/ki Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1-/- ki/ki
tissue: fetal liver
cell type: megakaryocyte
|
Meg_4397
|
| Sample_geo_accession | GSM741988
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741988/suppl/GSM741988.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
|
GSM741989 | GPL1261 |
|
FOG1+/+ Meg, biological rep3
|
FOG1+/+ Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1+/+
tissue: fetal liver
cell type: megakaryocyte
|
Meg_9482
|
| Sample_geo_accession | GSM741989
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741989/suppl/GSM741989.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
|
GSM741990 | GPL1261 |
|
FOG1-/- ki/ki Meg, biological rep3
|
FOG1-/- ki/ki Meg
|
background strain: mixed C57BL/6xSv129 background
genotype/variation: FOG1-/- ki/ki
tissue: fetal liver
cell type: megakaryocyte
|
Meg_9483
|
| Sample_geo_accession | GSM741990
| | Sample_status | Public on Jun 28 2011
| | Sample_submission_date | Jun 15 2011
| | Sample_last_update_date | Jun 28 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Mouse Megs were differentiated from fetal liver cells in IMDM medium with thrompoietin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from Megs using Rneasy Plus kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was amplified using the WT-Ovation Pico System from NuGEN. Sense transcript (ST)-cDNA was generated using the WT-Ovation Exon Module. ST-cDNA was subsequently fragmented and 3’ labeled with biotin using the WT-Ovation Biotin Module V2 (NuGEN).
| | Sample_hyb_protocol | Probes were hybridized to Affymetrix Mouse Genome 430 2.0 Array GeneChips.
| | Sample_scan_protocol | All samples were amplified, labeled, hybridized, and scanned at the Penn Microarray Facility (University of Pennsylvania) according to manufacturer’s protocol.
| | Sample_data_processing | The raw data (.CEL files) were processed by MAS5.0, using the xps package at the University of Pennsylvania Microarray Facility. The signals for the 3 replicates of each probe set were averaged. Averaged signals between Ki/Ki and WT samples were computed for comparative analyses (fold changes). Annotations were extracted from affymatrix database. Results and annotations were merged into Microsoft Excel.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuhuan,,Wang
| | Sample_contact_institute | The Children's Hospital of Philadelphia
| | Sample_contact_address | 3615 Civic Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM741nnn/GSM741990/suppl/GSM741990.CEL.gz
| | Sample_series_id | GSE29975
| | Sample_data_row_count | 45101
| | |
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