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Search results for the GEO ID: GSE30012

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM742803

GPL1261

Mouse Embryonic Stem Cells Control replicate 1

Dicerflox/flox wild-type embryonic stem cells transfected with GFP

genotype/variation: wild type (Dicer) cell type: embryonic stem cells transfection: GFP

Gene expression data from mouse embryoic stem cells with intact Dicer genes

Sample_geo_accession	GSM742803
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742803/suppl/GSM742803_CTRL1.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

GSM742804

GPL1261

Mouse Embryonic Stem Cells Control replicate 2

Dicerflox/flox wild-type embryonic stem cells transfected with GFP

genotype/variation: wild type (Dicer) cell type: embryonic stem cells transfection: GFP

Gene expression data from mouse embryoic stem cells with intact Dicer genes

Sample_geo_accession	GSM742804
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742804/suppl/GSM742804_CTRL2.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

GSM742805

GPL1261

Mouse Embryonic Stem Cells Control replicate 3

Dicerflox/flox wild-type embryonic stem cells transfected with GFP

genotype/variation: wild type (Dicer) cell type: embryonic stem cells transfection: GFP

Gene expression data from mouse embryoic stem cells with intact Dicer genes

Sample_geo_accession	GSM742805
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742805/suppl/GSM742805_CTRL3.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

GSM742806

GPL1261

Mouse Embryonic Stem Cells Dicer Null - replicate 1

Dicerflox/flox wild-type embryonic stem cells transfected with GFP and Cre Recombinase

genotype/variation: Dicer Null (Cre) cell type: embryonic stem cells transfection: GFP+Cre

Gene expression data from mouse embryoic stem cells following acute Dicer deletion

Sample_geo_accession	GSM742806
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742806/suppl/GSM742806_CRE1.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

GSM742807

GPL1261

Mouse Embryonic Stem Cells Dicer Null - replicate 2

Dicerflox/flox wild-type embryonic stem cells transfected with GFP and Cre Recombinase

genotype/variation: Dicer Null (Cre) cell type: embryonic stem cells transfection: GFP+Cre

Gene expression data from mouse embryoic stem cells following acute Dicer deletion

Sample_geo_accession	GSM742807
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742807/suppl/GSM742807_CRE2.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

GSM742808

GPL1261

Mouse Embryonic Stem Cells Dicer Null - replicate 3

Dicerflox/flox wild-type embryonic stem cells transfected with GFP and Cre Recombinase

genotype/variation: Dicer Null (Cre) cell type: embryonic stem cells transfection: GFP+Cre

Gene expression data from mouse embryoic stem cells following acute Dicer deletion

Sample_geo_accession	GSM742808
Sample_status	Public on Jun 18 2011
Sample_submission_date	Jun 15 2011
Sample_last_update_date	Jun 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	RNA was isolated 5 days following transfection of Dicerflox/flox wild-type cells with either GFP alone or GFP and Cre recombinase.
Sample_growth_protocol_ch1	ES cells were grown on gelatin in DMEM (GIBCO), 15% fetal calf serum (FCS, Hyclone), and 250 U LIF/ml (Y Marahrens, B Panning, J Dausman, W Strauss and R Jaenisch, Xist-deficient mice are defective in dosage compensation but not spermatogenesis, Genes Dev. 11 (1997), pp. 156–166) or 1000 U/ml when addressing the effect of Xist on undifferentiated ES cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
Sample_platform_id	GPL1261
Sample_contact_name	Charles,Arthur,Whittaker
Sample_contact_email	charliew@mit.edu
Sample_contact_institute	Koch Institute
Sample_contact_address	77 Mass Ave 76-189
Sample_contact_city	Cambridge
Sample_contact_state	MA
Sample_contact_zip/postal_code	02152
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM742nnn/GSM742808/suppl/GSM742808_CRE3.CEL.gz
Sample_series_id	GSE30012
Sample_data_row_count	45037

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