Search results for the GEO ID: GSE30141  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM328687 | GPL570 |
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DMSO control
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Breast cancer cell line MCF7
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perturbagen: small molecule, type: control, name: DMSO control, concentration: .00001 M, vehicle: DMSO, duration: 6 h, cell: MCF7
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Gene expression data from MCF7 cells treated only with DMSO for 6 hours.
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| Sample_geo_accession | GSM328687
| | Sample_status | Public on Oct 05 2009
| | Sample_submission_date | Oct 08 2008
| | Sample_last_update_date | Oct 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 control cells treated with DMSO for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Qiagen Rneasy minikit according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA) the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM328nnn/GSM328687/suppl/GSM328687.CEL.gz
| | Sample_series_id | GSE13124
| | Sample_series_id | GSE30141
| | Sample_data_row_count | 54675
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GSM746552 | GPL570 |
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NSC620358
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Breast cancer cell line MCF7
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perturbagen: small molecule
treatment: NSC620358
concentration: 0.00001 M
vehicle: DMSO
duration: 6 h
cell line: MCF7
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Gene expression data from MCF7 cells treated with NSC620358 for 6 hours.
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| Sample_geo_accession | GSM746552
| | Sample_status | Public on Jun 21 2013
| | Sample_submission_date | Jun 22 2011
| | Sample_last_update_date | Jun 21 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells treated with 10 microM NSC620358 for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Qiagen Rneasy minikit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA) the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM746nnn/GSM746552/suppl/GSM746552_MCF7_NSC620358_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE30141
| | Sample_data_row_count | 54675
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