Search results for the GEO ID: GSE30188  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM747347 | GPL570 |
|
DMSO_1
|
PC-3 prostate cancer 24hr DMSO
|
cell type: PC-3 prostate cancer
drug: DMSO control
|
Gene expression data from PC-3 prostate cancer cells treated with DMSO
|
| Sample_geo_accession | GSM747347
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747347/suppl/GSM747347_Neubig_001.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747348 | GPL570 |
|
DMSO_2
|
PC-3 prostate cancer 24hr DMSO
|
cell type: PC-3 prostate cancer
drug: DMSO control
|
Gene expression data from PC-3 prostate cancer cells treated with DMSO
|
| Sample_geo_accession | GSM747348
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747348/suppl/GSM747348_Neubig_002.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747349 | GPL570 |
|
DMSO_3
|
PC-3 prostate cancer 24hr DMSO
|
cell type: PC-3 prostate cancer
drug: DMSO control
|
Gene expression data from PC-3 prostate cancer cells treated with DMSO
|
| Sample_geo_accession | GSM747349
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747349/suppl/GSM747349_Neubig_003.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747351 | GPL570 |
|
CCG-1423_2hr_2
|
PC-3 prostate cancer 2hr CCG-1423
|
cell type: PC-3 prostate cancer
drug: N-[2-(4-chloroanilino)-1-methyl-2-oxoethoxy]-3,5-bis(trifluoromethyl) benzamide
|
Gene expression data from PC-3 prostate cancer cells treated with CCG-1423 for 2 hr
|
| Sample_geo_accession | GSM747351
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747351/suppl/GSM747351_Neubig_005.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747352 | GPL570 |
|
CCG-1423_2hr_3
|
PC-3 prostate cancer 2hr CCG-1423
|
cell type: PC-3 prostate cancer
drug: N-[2-(4-chloroanilino)-1-methyl-2-oxoethoxy]-3,5-bis(trifluoromethyl) benzamide
|
Gene expression data from PC-3 prostate cancer cells treated with CCG-1423 for 2 hr
|
| Sample_geo_accession | GSM747352
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747352/suppl/GSM747352_Neubig_006.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747353 | GPL570 |
|
LatB_2hr_1
|
PC-3 prostate cancer 2hr LatB
|
cell type: PC-3 prostate cancer
drug: Latrunculin B
|
Gene expression data from PC-3 prostate cancer cells treated with Latrunculin B for 2 hr
|
| Sample_geo_accession | GSM747353
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747353/suppl/GSM747353_Neubig_007.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747354 | GPL570 |
|
LatB_2hr_2
|
PC-3 prostate cancer 2hr LatB
|
cell type: PC-3 prostate cancer
drug: Latrunculin B
|
Gene expression data from PC-3 prostate cancer cells treated with Latrunculin B for 2 hr
|
| Sample_geo_accession | GSM747354
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747354/suppl/GSM747354_Neubig_008.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747355 | GPL570 |
|
LatB_2hr_3
|
PC-3 prostate cancer 2hr LatB
|
cell type: PC-3 prostate cancer
drug: Latrunculin B
|
Gene expression data from PC-3 prostate cancer cells treated with Latrunculin B for 2 hr
|
| Sample_geo_accession | GSM747355
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747355/suppl/GSM747355_Neubig_009.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747356 | GPL570 |
|
DRB_2hr_1
|
PC-3 prostate cancer 2hr DRB
|
cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
|
Gene expression data from PC-3 prostate cancer cells treated with DRB for 2 hr
|
| Sample_geo_accession | GSM747356
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747356/suppl/GSM747356_Neubig_010.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747357 | GPL570 |
|
DRB_2hr_2
|
PC-3 prostate cancer 2hr DRB
|
cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
|
Gene expression data from PC-3 prostate cancer cells treated with DRB for 2 hr
|
| Sample_geo_accession | GSM747357
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747357/suppl/GSM747357_Neubig_011.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747358 | GPL570 |
|
DRB_2hr_3
|
PC-3 prostate cancer 2hr DRB
|
cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
|
Gene expression data from PC-3 prostate cancer cells treated with DRB for 2 hr
|
| Sample_geo_accession | GSM747358
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747358/suppl/GSM747358_Neubig_012.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747359 | GPL570 |
|
CCG-1423_24hr_1
|
PC-3 prostate cancer 24hr CCG-1423
|
cell type: PC-3 prostate cancer
drug: N-[2-(4-chloroanilino)-1-methyl-2-oxoethoxy]-3,5-bis(trifluoromethyl) benzamide
|
Gene expression data from PC-3 prostate cancer cells treated with CCG-1423 for 24 hr
|
| Sample_geo_accession | GSM747359
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747359/suppl/GSM747359_Neubig_013.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747360 | GPL570 |
|
CCG-1423_24hr_2
|
PC-3 prostate cancer 24hr CCG-1423
|
cell type: PC-3 prostate cancer
drug: N-[2-(4-chloroanilino)-1-methyl-2-oxoethoxy]-3,5-bis(trifluoromethyl) benzamide
|
Gene expression data from PC-3 prostate cancer cells treated with CCG-1423 for 24 hr
|
| Sample_geo_accession | GSM747360
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747360/suppl/GSM747360_Neubig_014.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747361 | GPL570 |
|
CCG-1423_24hr_3
|
PC-3 prostate cancer 24hr CCG-1423
|
cell type: PC-3 prostate cancer
drug: N-[2-(4-chloroanilino)-1-methyl-2-oxoethoxy]-3,5-bis(trifluoromethyl) benzamide
|
Gene expression data from PC-3 prostate cancer cells treated with CCG-1423 for 24 hr
|
| Sample_geo_accession | GSM747361
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747361/suppl/GSM747361_Neubig_015.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747362 | GPL570 |
|
LatB_24hr_1
|
PC-3 prostate cancer 24hr LatB
|
cell type: PC-3 prostate cancer
drug: Latrunculin B
|
Gene expression data from PC-3 prostate cancer cells treated with Latrunculin B for 24 hr
|
| Sample_geo_accession | GSM747362
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747362/suppl/GSM747362_Neubig_016.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747363 | GPL570 |
|
LatB_24hr_2
|
PC-3 prostate cancer 24hr LatB
|
cell type: PC-3 prostate cancer
drug: Latrunculin B
|
Gene expression data from PC-3 prostate cancer cells treated with Latrunculin B for 24 hr
|
| Sample_geo_accession | GSM747363
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747363/suppl/GSM747363_Neubig_018.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747364 | GPL570 |
|
DRB_24hr_1
|
PC-3 prostate cancer 24hr DRB
|
cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
|
Gene expression data from PC-3 prostate cancer cells treated with DRB for 24 hr
|
| Sample_geo_accession | GSM747364
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747364/suppl/GSM747364_Neubig_019.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
| | |
|
GSM747365 | GPL570 |
|
DRB_24hr_2
|
PC-3 prostate cancer 24hr DRB
|
cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
|
Gene expression data from PC-3 prostate cancer cells treated with DRB for 24 hr
|
| Sample_geo_accession | GSM747365
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747365/suppl/GSM747365_Neubig_020.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
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GSM747366 | GPL570 |
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DRB_24hr_3
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PC-3 prostate cancer 24hr DRB
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cell type: PC-3 prostate cancer
drug: 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole
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Gene expression data from PC-3 prostate cancer cells treated with DRB for 24 hr
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| Sample_geo_accession | GSM747366
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Jun 24 2011
| | Sample_last_update_date | May 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At t=0 hr or t=22 hr, DMSO (1%) or compounds (CCG-1423 3 uM, Latrunculin B 0.5 uM, or DRB 50 uM) were added . Cells were harvested at t=24 hr. Samples treated for 2 hr (CCG-1423, LatB, or DRB) or 24 hr (DMSO, CCG-1423, LatB, or DRB) were all compared to results for the 24 hr DMSO treatment.
| | Sample_growth_protocol_ch1 | Triplicate cultures of human PC-3 prostate cancer cells (9 x 10^5) in 60 mm dishes were grown in 10% FBS for 24 hours then were serum starved for 24 hours (0.5% FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RNAqueous® kit from Ambion.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values
| | Sample_platform_id | GPL570
| | Sample_contact_name | Richard,R.,Neubig
| | Sample_contact_email | rneubig@umich.edu
| | Sample_contact_phone | 734 764-8165
| | Sample_contact_fax | 7347648165
| | Sample_contact_department | Pharmacology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Med. Ctr. Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5632
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://warbler.med.umich.edu
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM747nnn/GSM747366/suppl/GSM747366_Neubig_021.CEL.gz
| | Sample_series_id | GSE30188
| | Sample_data_row_count | 54675
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