Search results for the GEO ID: GSE30304  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM751142 | GPL570 |
|
RWPE-1,2D,triplicate data,01
|
RWPE-1,2D
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 2D culture
|
RWPE-1-2D-1
|
| Sample_geo_accession | GSM751142
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751142/suppl/GSM751142.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751142/suppl/GSM751142.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751143 | GPL570 |
|
RWPE-1,2D,triplicate data,02
|
RWPE-1,2D
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 2D culture
|
RWPE-1-2D-2
|
| Sample_geo_accession | GSM751143
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751143/suppl/GSM751143.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751143/suppl/GSM751143.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751144 | GPL570 |
|
RWPE-1,2D,triplicate data,03
|
RWPE-1,2D
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 2D culture
|
RWPE-1-2D-3
|
| Sample_geo_accession | GSM751144
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751144/suppl/GSM751144.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751144/suppl/GSM751144.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751146 | GPL570 |
|
RWPE-1,3D culture for 2day,triplicate data,02
|
RWPE-1,3D culture for 2day
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 3D culture
time: 2days
|
RWPE-1-3Dcluster-2
|
| Sample_geo_accession | GSM751146
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751146/suppl/GSM751146.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751146/suppl/GSM751146.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751147 | GPL570 |
|
RWPE-1,3D culture for 2day,triplicate data,03
|
RWPE-1,3D culture for 2day
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 3D culture
time: 2days
|
RWPE-1-3Dcluster-3
|
| Sample_geo_accession | GSM751147
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751147/suppl/GSM751147.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751147/suppl/GSM751147.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751148 | GPL570 |
|
RWPE-1,3D culture for 6day,triplicate data,01
|
RWPE-1,3D culture for 6day
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 3D culture
time: 6days
|
RWPE-1-3Dacini-1
|
| Sample_geo_accession | GSM751148
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751148/suppl/GSM751148.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751148/suppl/GSM751148.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751149 | GPL570 |
|
RWPE-1,3D culture for 6day,triplicate data,02
|
RWPE-1,3D culture for 6day
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 3D culture
time: 6days
|
RWPE-1-3Dacini-2
|
| Sample_geo_accession | GSM751149
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751149/suppl/GSM751149.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751149/suppl/GSM751149.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751150 | GPL570 |
|
RWPE-1,3D culture for 6day,triplicate data,03
|
RWPE-1,3D culture for 6day
|
tissue: Prostate
cell line: RWPE-1 (immortalized prostate epithelial cells)
growth protocol: 3D culture
time: 6days
|
RWPE-1-3Dacini-3
|
| Sample_geo_accession | GSM751150
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751150/suppl/GSM751150.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751150/suppl/GSM751150.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751151 | GPL570 |
|
LNCAP,2D,triplicate data,01
|
LNCAP,2D
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 2D culture
|
LNCAP-2D-1
|
| Sample_geo_accession | GSM751151
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751151/suppl/GSM751151.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751151/suppl/GSM751151.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751152 | GPL570 |
|
LNCAP,2D,triplicate data,02
|
LNCAP,2D
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 2D culture
|
LNCAP-2D-2
|
| Sample_geo_accession | GSM751152
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751152/suppl/GSM751152.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751152/suppl/GSM751152.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751153 | GPL570 |
|
LNCAP,2D,triplicate data,03
|
LNCAP,2D
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 2D culture
|
LNCAP-2D-3
|
| Sample_geo_accession | GSM751153
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751153/suppl/GSM751153.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751153/suppl/GSM751153.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751154 | GPL570 |
|
LNCAP,3D culture for 2day,triplicate data,01
|
LNCAP,3D culture for 2day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 2days
|
LNCAP-3Dcluster-1
|
| Sample_geo_accession | GSM751154
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751154/suppl/GSM751154.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751154/suppl/GSM751154.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751155 | GPL570 |
|
LNCAP,3D culture for 2day,triplicate data,02
|
LNCAP,3D culture for 2day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 2days
|
LNCAP-3Dcluster-2
|
| Sample_geo_accession | GSM751155
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751155/suppl/GSM751155.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751155/suppl/GSM751155.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751156 | GPL570 |
|
LNCAP,3D culture for 2day,triplicate data,03
|
LNCAP,3D culture for 2day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 2days
|
LNCAP-3Dcluster-3
|
| Sample_geo_accession | GSM751156
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751156/suppl/GSM751156.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751156/suppl/GSM751156.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751157 | GPL570 |
|
LNCAP,3D culture for 6day,triplicate data,01
|
LNCAP,3D culture for 6day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 6days
|
LNCAP-3Dspheroid-1
|
| Sample_geo_accession | GSM751157
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751157/suppl/GSM751157.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751157/suppl/GSM751157.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751158 | GPL570 |
|
LNCAP,3D culture for 6day,triplicate data,02
|
LNCAP,3D culture for 6day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 6days
|
LNCAP-3Dspheroid-2
|
| Sample_geo_accession | GSM751158
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751158/suppl/GSM751158.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751158/suppl/GSM751158.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
GSM751159 | GPL570 |
|
LNCAP,3D culture for 6day,triplicate data,03
|
LNCAP,3D culture for 6day
|
tissue: Prostate
cell line: LNCaP cells (prostate cancer cells)
growth protocol: 3D culture
time: 6days
|
LNCAP-3Dspheroid-3
|
| Sample_geo_accession | GSM751159
| | Sample_status | Public on Jul 03 2011
| | Sample_submission_date | Jun 29 2011
| | Sample_last_update_date | Jul 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | RWPE-1 cells were maintained in Keratinocyte-SFM and LNCAP cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from 2D,3D-2days,and 3D-6days using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jimmy,Jiun-Ming,Su
| | Sample_contact_email | jimmy.su.js@gmail.com
| | Sample_contact_phone | +886-6-208-3422 65246
| | Sample_contact_department | National Institute of Cancer Research
| | Sample_contact_institute | National Health Research Institutes,Taiwan
| | Sample_contact_address | 1F,No 367,Sheng-Li Rd. North District
| | Sample_contact_city | Tainan city
| | Sample_contact_state | n/a
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | none
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751159/suppl/GSM751159.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM751nnn/GSM751159/suppl/GSM751159.CHP.gz
| | Sample_series_id | GSE30304
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
