Search results for the GEO ID: GSE30356  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM753450 | GPL1261 |
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FAK knockout, endothelin-1 treated
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knock out, endothelin-1 treated, embryonic fibroblast
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cell type: embryonic fibroblast
treatment: Endothelin-1
genotype: FAK -/-
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Gene expression data from knockout embryonic fibroblasts
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| Sample_geo_accession | GSM753450
| | Sample_status | Public on Jul 01 2012
| | Sample_submission_date | Jul 01 2011
| | Sample_last_update_date | Jul 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | wild type and knock out cells at 50% confluence were starved for 24h in serum-free medium, then treated with or without endothelin-1 for 24h.
| | Sample_growth_protocol_ch1 | Mouse embryonic fibroblasts taken from FAK (focal adhesion kinase) +/+ and FAK -/- mice were grown in DMEM media containing 10% FBS, 2mM L-glutamine and antibiotics (100U/mL penicillin and 100 µg/mL streptomycin). Cells were grown at 37°C, 5%CO2 and used at passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the two-cycle Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneSpring GX using the RMA normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,E,Carter
| | Sample_contact_email | dcarter@robarts.ca
| | Sample_contact_department | London Regional Genomics Centre
| | Sample_contact_institute | Robarts Research Institute
| | Sample_contact_address | 100 Perth Drive
| | Sample_contact_city | London
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | N6A5K8
| | Sample_contact_country | Canada
| | Sample_contact_web_link | www.lrgc.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM753nnn/GSM753450/suppl/GSM753450_AFFY1384.CEL.gz
| | Sample_series_id | GSE30356
| | Sample_data_row_count | 45101
| | |
|
GSM753451 | GPL1261 |
|
wild type, endothelin-1 treated
|
wild type, endothelin-1 treated, embryonic fibroblast
|
cell type: embryonic fibroblast
treatment: Endothelin-1
genotype: FAK +/+
|
Gene expression data from WT embryonic fibroblasts
|
| Sample_geo_accession | GSM753451
| | Sample_status | Public on Jul 01 2012
| | Sample_submission_date | Jul 01 2011
| | Sample_last_update_date | Jul 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | wild type and knock out cells at 50% confluence were starved for 24h in serum-free medium, then treated with or without endothelin-1 for 24h.
| | Sample_growth_protocol_ch1 | Mouse embryonic fibroblasts taken from FAK (focal adhesion kinase) +/+ and FAK -/- mice were grown in DMEM media containing 10% FBS, 2mM L-glutamine and antibiotics (100U/mL penicillin and 100 µg/mL streptomycin). Cells were grown at 37°C, 5%CO2 and used at passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the two-cycle Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneSpring GX using the RMA normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,E,Carter
| | Sample_contact_email | dcarter@robarts.ca
| | Sample_contact_department | London Regional Genomics Centre
| | Sample_contact_institute | Robarts Research Institute
| | Sample_contact_address | 100 Perth Drive
| | Sample_contact_city | London
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | N6A5K8
| | Sample_contact_country | Canada
| | Sample_contact_web_link | www.lrgc.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM753nnn/GSM753451/suppl/GSM753451_AFFY1385.CEL.gz
| | Sample_series_id | GSE30356
| | Sample_data_row_count | 45101
| | |
|
GSM753452 | GPL1261 |
|
FAK knockout, not treated
|
knock out, not treated, embryonic fibroblast
|
cell type: embryonic fibroblast
treatment: untreated
genotype: FAK -/-
|
Gene expression data from knockout embryonic fibroblasts
|
| Sample_geo_accession | GSM753452
| | Sample_status | Public on Jul 01 2012
| | Sample_submission_date | Jul 01 2011
| | Sample_last_update_date | Jul 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | wild type and knock out cells at 50% confluence were starved for 24h in serum-free medium, then treated with or without endothelin-1 for 24h.
| | Sample_growth_protocol_ch1 | Mouse embryonic fibroblasts taken from FAK (focal adhesion kinase) +/+ and FAK -/- mice were grown in DMEM media containing 10% FBS, 2mM L-glutamine and antibiotics (100U/mL penicillin and 100 µg/mL streptomycin). Cells were grown at 37°C, 5%CO2 and used at passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the two-cycle Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneSpring GX using the RMA normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,E,Carter
| | Sample_contact_email | dcarter@robarts.ca
| | Sample_contact_department | London Regional Genomics Centre
| | Sample_contact_institute | Robarts Research Institute
| | Sample_contact_address | 100 Perth Drive
| | Sample_contact_city | London
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | N6A5K8
| | Sample_contact_country | Canada
| | Sample_contact_web_link | www.lrgc.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM753nnn/GSM753452/suppl/GSM753452_AFFY1386.CEL.gz
| | Sample_series_id | GSE30356
| | Sample_data_row_count | 45101
| | |
|
GSM753453 | GPL1261 |
|
wild type, not treated
|
wild type, not treated, embryonic fibroblast
|
cell type: embryonic fibroblast
treatment: untreated
genotype: FAK +/+
|
Gene expression data from WT embryonic fibroblasts
|
| Sample_geo_accession | GSM753453
| | Sample_status | Public on Jul 01 2012
| | Sample_submission_date | Jul 01 2011
| | Sample_last_update_date | Jul 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | wild type and knock out cells at 50% confluence were starved for 24h in serum-free medium, then treated with or without endothelin-1 for 24h.
| | Sample_growth_protocol_ch1 | Mouse embryonic fibroblasts taken from FAK (focal adhesion kinase) +/+ and FAK -/- mice were grown in DMEM media containing 10% FBS, 2mM L-glutamine and antibiotics (100U/mL penicillin and 100 µg/mL streptomycin). Cells were grown at 37°C, 5%CO2 and used at passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the two-cycle Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneSpring GX using the RMA normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,E,Carter
| | Sample_contact_email | dcarter@robarts.ca
| | Sample_contact_department | London Regional Genomics Centre
| | Sample_contact_institute | Robarts Research Institute
| | Sample_contact_address | 100 Perth Drive
| | Sample_contact_city | London
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | N6A5K8
| | Sample_contact_country | Canada
| | Sample_contact_web_link | www.lrgc.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM753nnn/GSM753453/suppl/GSM753453_AFFY1387.CEL.gz
| | Sample_series_id | GSE30356
| | Sample_data_row_count | 45101
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